【摘要】：目的：糖尿病可以誘發(fā)視網(wǎng)膜細胞凋亡,同時誘發(fā)神經(jīng)系統(tǒng)、血漿出現(xiàn)感覺神經(jīng)遞質(zhì)降鈣素基因相關(guān)肽(calcitonin gene-related peptide, CGRP)和P物質(zhì)(substance P, SP)的耗損,辣椒素可引起CGRP和SP等感覺神經(jīng)遞質(zhì)釋放并且參與器官保護,然而CGRP和SP在糖尿病視網(wǎng)膜病變細胞凋亡中的作用如何尚待闡明。觀察分析鏈脲佐菌素(streptozocin, STZ)誘發(fā)的糖尿病視網(wǎng)膜病變(Diabetic Retinopathy, DR)大鼠視網(wǎng)膜細胞病理學改變及凋亡與視網(wǎng)膜和血清中感覺神經(jīng)遞質(zhì)CGRP和SP變化的關(guān)系；探討辣椒素預處理對糖尿病視網(wǎng)膜病變大鼠視網(wǎng)膜組織和血清中CGRP和SP的表達變化及其對糖尿病視網(wǎng)膜細胞凋亡的保護作用。 方法：將健康成年雄性SD大鼠40只(80只眼)隨機分為5組,每組8只(16眼)。DR組：1%STZ溶液腹腔注射給藥；藥物干預組：前三天皮下注射1%辣椒素溶液(20mg/kg),第四天腹腔注射1%STZ溶液；DR對照組和藥物干預對照組分別給予上述藥物的等量溶劑；空白對照組：不做任何處理。糖尿病大鼠造模后飼養(yǎng)10周,組織病理切片證實糖尿病視網(wǎng)膜病變大鼠模型造模成功。檢測各組視網(wǎng)膜細胞凋亡率及caspase-3(?)舌性,視網(wǎng)膜和血清中CGRP和SP的表達變化。 結(jié)果：DR組的視網(wǎng)膜神經(jīng)節(jié)層細胞凋亡率(43.4%±5.0%)高于藥物干預組(30.0%±5.1%),差異有統(tǒng)計學意義(t=5.930,P0.01)；DR對照組和藥物干預對照組的視網(wǎng)膜細胞凋亡率分別為(12.4%±9.9%,17.6%±6.1%),與各自實驗組比較,差異有統(tǒng)計學意義(t=8.800,t=4.925,P0.01)。空白組細胞凋亡率為(16.2%±6.9%),與兩個對照組比較,差異無統(tǒng)計學意義(t=-0.989, t=0.951, P0.05). DR組大鼠視網(wǎng)膜中caspase-3比活性值為(2.19±0.86),藥物干預組為(1.96±0.56),差異有統(tǒng)計學意義(t=-0.515,P0.05)。DR對照組和藥物干預對照組的比活性值分別為(1.47±0.14)和(0.74±0.27),與各自實驗組比較,差異有統(tǒng)計學意義(t=2.797,t=2.142, P0.05)。DR組視網(wǎng)膜CGRP含量為(424.4±44.2pg/ml),藥物干預組為(543.2±74.4pg/ml),兩組比較差異有統(tǒng)計學意義(t=3.070,P0.05); DR組血清CGRP含量為(148.8±39.1pg/ml),藥物干預組血清CGRP含量(237.5±78.7pg/ml),差異有統(tǒng)計學意義(t=2.359,P0.05)。DR組視網(wǎng)膜SP含量為(20.42±0.94ng/ml),藥物干預組為(24.08±3.34ng/ml),兩組比較差異有統(tǒng)計學意義(t=2.142,P0.05); DR對照組視網(wǎng)膜SP含量為(26.69±7.74ng/ml),與DR組比較差異有統(tǒng)計學意義(t=-1.587,P0.05); DR組血清SP含量為(10.32±1.14ng/ml),藥物干預組血清SP含量為(12.89±1.52ng/ml),差異有統(tǒng)計學意義(t=3.017,P0.05); DR對照組血清SP含量為(12.24±0.63ng/ml),與DR組比較差異有統(tǒng)計學意義(t=-3.554,P0.05) 結(jié)論：DR可引起視網(wǎng)膜神經(jīng)節(jié)細胞凋亡顯著增加,視網(wǎng)膜組織和血清CGRP和SP水平的下降,小劑量辣椒素可誘導糖尿病視網(wǎng)膜組織和血清CGRP和SP水平上調(diào),減輕視網(wǎng)膜細胞凋亡,對抗DR引起的視網(wǎng)膜組織損傷。提示糖尿病大鼠視網(wǎng)膜組織和血清CGRP和SP水平的下降可能與DR引起視網(wǎng)膜神經(jīng)節(jié)細胞凋亡增加有關(guān),CGRP和SP可能參與對DR引起的視網(wǎng)膜細胞凋亡的保護作用。
[Abstract]:Objective: diabetes can induce apoptosis of retina cells and induce the nervous system, and the loss of calcitonin gene-related peptide (CGRP) and P substance (substance P, SP) in plasma, capsaicin can cause the release of sensory neurotransmitters such as CGRP and SP and participate in organ protection. The role of SP in the apoptosis of diabetic retinopathy remains to be elucidated. The relationship between the pathological changes of retinal cell pathology and the relationship between apoptosis and the changes of the sensory neurotransmitters CGRP and SP in the retina and serum of the diabetic retinopathy (Diabetic Retinopathy, DR) induced diabetic retinopathy (Diabetic Retinopathy, DR) induced by streptozotocin (STZ) was investigated. Capsaicin preconditioning protects the expression of CGRP and SP in the retina tissue and serum of diabetic retinopathy rats and the protective effect of capsaicin on the apoptosis of diabetic retina cells.
Methods: 40 healthy adult male SD rats (80 eyes) were randomly divided into 5 groups, each group of 8 (16 eyes) group.DR: 1%STZ solution intraperitoneal injection; drug intervention group: the first three days the subcutaneous injection of 1% capsaicin solution (20mg/kg), fourth days intraperitoneal injection of 1%STZ solution; DR control group and drug intervention control group to give the same amount of drugs to the same amount, respectively. In the blank control group, no treatment was done in the control group. The diabetic rat model was fed for 10 weeks. The histopathological section confirmed the success of the diabetic retinopathy model. The apoptosis rate of retinal cells and the caspase-3 (?) tongue, the expression of CGRP and SP in the retina and serum were detected.
Results: the apoptosis rate of retinal ganglion layer cells in DR group (43.4% + 5%) was higher than that of the drug intervention group (30% + 5.1%), and the difference was statistically significant (t=5.930, P0.01). The apoptosis rate of retinal cells in the DR control group and the drug intervention control group were (12.4% + 9.9%, 17.6% + 6.1%) respectively, and the difference was statistically significant (t=8.800, t). =4.925, P0.01). The cell apoptosis rate in the blank group was (16.2% + 6.9%). Compared with the two control groups, the difference was not statistically significant (t=-0.989, t=0.951, P0.05). The caspase-3 ratio activity in the retina of the DR group was (2.19 + 0.86), and the drug intervention group was (1.96 + 0.56), the difference was statistically significant (t=-0.515, P0.05).DR control group and drug intervention control group The specific activity values were (1.47 + 0.14) and (0.74 + 0.27) respectively. Compared with the experimental group, the difference was statistically significant (t=2.797, t=2.142, P0.05).DR group CGRP content was (424.4 + 44.2pg/ml), the drug intervention group was (543.2 + 74.4pg/ml), the two groups were statistically significant (t=3.070, P0.05); DR group serum CGRP content was (148.8 + 39.1p). G/ml), the serum CGRP content of the drug intervention group (237.5 + 78.7pg/ml), the difference was statistically significant (t=2.359, P0.05).DR group retinal SP content was (20.42 + 0.94ng/ml), the drug intervention group was (24.08 + 3.34ng/ml), the two groups were statistically significant (t=2.142, P0.05), and the retina content of the retina was (26.69 +), and was worse than that of the group. The difference was statistically significant (t=-1.587, P0.05); the serum SP content in DR group was (10.32 + 1.14ng/ml), and the serum SP content of the drug intervention group was (12.89 + 1.52ng/ml), the difference was statistically significant (t=3.017, P0.05), and the SP content of the serum of DR control group was (12.24 + 0.63ng/ml).
Conclusion: DR can cause a significant increase in retinal ganglion cell apoptosis, the decrease of CGRP and SP levels in retinal tissue and serum. Small dose capsaicin can induce the up-regulation of CGRP and SP levels in diabetic retina tissue and serum, alleviate retinal cell apoptosis and antagonism the damage of retinal membrane tissue caused by DR, suggesting the retina tissue of diabetic rats The decrease of CGRP and SP levels in serum may be associated with increased apoptosis of retinal ganglion cells caused by DR, and CGRP and SP may be involved in the protection of retinal cell apoptosis induced by DR.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R774.1

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