【摘要】：目的探究羊膜淚道支架對雄兔干眼癥的預(yù)防效果。方法選取36只健康雄兔,剪除第三眼瞼并抗感染處理,隨機分為3組(每組12只),制作去勢雄兔模型,其中A組為陰性對照組,B組為干眼模型組,C組為淚道羊膜預(yù)防組,并在植入羊膜淚道支架前、植入后2周、4周及6周行角膜熒光素(fluorescent,FL)染色、Schirmer I試驗、Western blot、免疫熒光染色及角膜共聚焦顯微鏡檢查。結(jié)果三組不同時間點間的淚液分泌量及FL有明顯改變(F=7.126,P=0.009;F=9.658,P=0.016),三組間淚液分泌量及FL有明顯改變(F=12.582,P=0.005;F=13.187,P=0.013),三組的淚液分泌量及FL變化趨勢也有明顯改變(F=8.531,P=0.007;F=10.652,P=0.019)。A、B、C三組植入后6周上皮基底細胞分別(3811±414)個細胞、(3820±314)個細胞、(2789±353)個細胞,炎癥細胞密度分別為(266±28)個細胞、(266±29)個細胞、(67±13)個細胞;A、B、C三組植入后6周上皮基底細胞(F=13.442,P=0.012)和炎癥細胞密度(F=9.231,P=0.021)比較,差異均有統(tǒng)計學意義。淚道上皮K16染色均為陰性,植入羊膜淚道支架后各時間點A、B、C三組兔淚小管組織中α-SMA表達無明顯改變,差異有統(tǒng)計學意義(F=14.681,P=0.002)。結(jié)論植入羊膜淚道支架對雄兔干眼癥的預(yù)防有一定的效果。
[Abstract]:Objective to investigate the preventive effect of amniotic lacrimal duct stent on dry eye in male rabbits. Methods Thirty-six healthy male rabbits were randomly divided into 3 groups (12 in each group), with the third eyelid being cut off and treated with anti-infection. The ovariectomized male rabbit model was established in which group A was negative control group and group B was dry eye model group, group C was lacrimal duct amniotic membrane prophylaxis group. Before and after implantation of amniotic lacrimal duct stent, fluorescentin FL staining and Western blot, immunofluorescence staining and corneal confocal microscopy were performed 2 weeks and 6 weeks after implantation. Results there were significant changes in tear secretion and FL in the three groups at different time points (FF7.126P0.009 / F9.658 / P0. 016). There were significant changes in tear secretion and FL between the three groups (FF12. 582PU 0. 005 FN 13.187 P0.013). There was also a significant change in tear secretion and FL in the three groups (FF8. 531P0. 007FN 10. 652P0.019). ABC group 3 had a significant change in the epithelium of the epithelium 6 weeks after implantation (FF8. 531P0. 007FN 10.652P0. 019). Basal cells were (3811 鹵414) cells, (3820 鹵314) cells and (2789 鹵353) cells, respectively. The density of inflammatory cells was (266 鹵28) cells, (266 鹵29) cells, and (67 鹵13) cells, respectively. There were significant differences in epithelial basal cells (F _ (13.442) P _ (0.012) and inflammatory cell densities (F _ (9.231) P _ (0.021) 6 weeks after implantation. The expression of 偽 -SMA in lacrimal canaliculus tissues of the three groups was not significantly changed after the implantation of amniotic lacrimal duct stents at all time points, and the difference was statistically significant (F _ (14.681) P ~ (0.002). Conclusion the implantation of amniotic lacrimal duct stent has certain effect on the prevention of xerophthalmia in male rabbits.
【作者單位】： 南昌大學第一附屬醫(yī)院眼科;
【基金】：國家自然科學基金資助(編號:81660158、81160118、81160148、81460092、81400372、81400424) 江西省遠航工程(編號:2014022) 江西省自然科學基金重大項目(編號:2016ACB21017) 江西省青年科學基金(編號:20151BAB215016、20161BAB215198) 江西省重點研發(fā)項目(編號:20151BBG70223) 江西省教育廳重點項目(編號:GJJ160020);江西省教育廳科技計劃項目(編號:GJJ160122) 江西省衛(wèi)計委科技計劃項目(編號:20164017、20175115、20175116);江西省衛(wèi)計委中醫(yī)藥科技計劃項目(編號:20150823)~~
【分類號】：R777.34
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本文編號：2146430