發(fā)布時間：2018-07-12 21:53

  本文選題：慢性鼻鼻竇炎 + 中鼻道外側(cè)壁粘膜上皮細(xì)胞　； 參考：《華中科技大學(xué)》2012年碩士論文

【摘要】：【目的】 通過對慢性鼻-鼻竇炎粘膜上皮細(xì)胞中呼吸道病毒的定性檢測，觀察呼吸道病毒在慢性鼻鼻竇炎持續(xù)期中是否存在及存在種類的差別，以期發(fā)現(xiàn)病毒在此疾病中的作用。 【材料和方法】 Control組病例共計27例，男性19例，女性8例，平均年齡32.1歲，病程從6天～十余年；CRSsNP組病例共計30例，男性22例，女性8例，平均年齡58.7歲，病程三月余～20余年；CRSwNP組病例共計35例，男性25例，女性10例，平均年齡37.4歲，病程半年余～20余年；其中伴血嗜酸性粒細(xì)胞增高（Eo增高）10例，不伴嗜酸性粒細(xì)胞增高（Eo正常）25例。對符合入組條件病例，在鼻內(nèi)鏡下用鼻粘膜刮匙刮取中鼻道外側(cè)壁粘膜上皮細(xì)胞，上皮細(xì)胞提取RNA后用聚合酶鏈反應(yīng)（PCR）技術(shù)行常見呼吸道病毒檢測。 【結(jié)果】 在92名患者中，檢測到總病毒陽性率為71.7%，但三組之間無差異，分別為77.8%、70%、68.6%。各種病毒構(gòu)成比中RV的總體陽性率最高，為33%，其次是Inf-A（20%），然后依次為Inf-B和PIV-1（均為15%）、RSV（13%）、OC43(12%)、PIV3（10%），PIV-2和229E陽性率較低，為3%。但是在三組患者之間每種病毒陽性率未發(fā)現(xiàn)統(tǒng)計學(xué)差異結(jié)論，除了PIV-2和229E只存在于Control組。PIV-1在Eo增高組（40%）和Eo正常組（4%）有顯著統(tǒng)計學(xué)差異。男性患者在各組之間的陽性率均比女性為高。 【結(jié)論】 1、檢測的總的病毒陽性率為71.7%，在各種陽性病毒中，RV的總體陽性率最高，但在三組之間無明顯統(tǒng)計學(xué)差異，提示在正常情況下鼻腔粘膜即定植大量病毒，其中鼻病毒是最常見類型，但慢性鼻-鼻竇炎并不比正常人對呼吸道病毒更易感，病毒也不在慢性鼻-鼻竇炎持續(xù)期的病理機理中起重要作用。 2、病毒多重陽性率在總的陽性結(jié)果中占相當(dāng)大的比例（41/66），超過一半為多重病毒感染，但在三組之間并無差別，提示我們多種病毒同時存在于鼻腔內(nèi)應(yīng)該屬于自然常態(tài)，單獨的病毒共存狀態(tài)并不增加其致病性。 3、各組男性病人檢測的病毒陽性率均比女性為高，提示呼吸道病毒可能更易定植于男性，男、女的易感病毒種類也有所差異，但并不造成更多的顯性感染。
[Abstract]:[objective] to investigate the existence and type of respiratory viruses in chronic rhinosinusitis by qualitative detection of respiratory viruses in mucosal epithelial cells of chronic rhinosinusitis. [materials and methods] there were 27 cases in the Control group, 19 males and 8 females, with an average age of 32.1 years. The course of disease ranged from 6 days to more than 10 years in 30 cases of CRSsNP group. There were 22 males and 8 females with an average age of 58.7 years. There were 35 cases in CRSwNP group in more than 20 years after three months. There were 25 males and 10 females with an average age of 37.4 years. The course of disease was more than half a year. There were 10 cases with eosinophil elevation (Eo) and 25 cases without eosinophil elevation (Eo normal). The epithelial cells of the lateral wall of the middle nasal canal were curetted with nasal mucosal curette under nasal endoscope for the patients who fit the condition of entry. RNA was extracted from epithelial cells and detected by polymerase chain reaction (PCR). [results] in 92 patients, the positive rate of total virus was 71.7, but there was no difference among the three groups, which were 77.8% and 68.6%, respectively. The overall positive rate of RV was 33, followed by Inf-A (20%), followed by Inf-B and PIV-1 (15%), RSV (13%), OC43 (12%), PIV3 (10%), PIV-2 and 229E (329E). However, there was no significant difference in the positive rate of each virus among the three groups, except that PIV-2 and 229E only existed in Control group. PIV-1 was significantly different in Eo elevated group (40%) and Eo normal group (4%). The positive rate of male patients was higher than that of females. [conclusion] 1. The total positive rate of virus detected was 71.7 and the positive rate of RV was the highest among all kinds of positive viruses. But there was no significant statistical difference among the three groups, suggesting that a large number of viruses were colonized in the nasal mucosa under normal conditions, of which rhinovirus was the most common type, but chronic rhinosinusitis was not more susceptible to respiratory viruses than normal people. The virus also does not play an important role in the pathological mechanism of chronic rhinosinusitis. 2. The multiple positive rate of the virus accounts for a large proportion of the total positive results (41 / 66), and more than half are multiplex virus infections. But there is no difference among the three groups, suggesting that multiple viruses should exist in the nasal cavity at the same time as the natural normal. The coexistence of viruses alone did not increase their pathogenicity. 3. The positive rate of viruses detected by male patients in each group was higher than that of women, suggesting that respiratory viruses may be more susceptible to infection in men, men and women, and there are also differences in the types of susceptible viruses. But it does not cause more dominant infections.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R765.41

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 朱毅,殷凱生;呼吸道病毒感染與哮喘[J];國外醫(yī)學(xué).呼吸系統(tǒng)分冊;2004年06期

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本文編號：2118577