發(fā)布時(shí)間：2018-07-08 19:35

  本文選題：AIFM + 耳蝸電圖　； 參考：《中華耳科學(xué)雜志》2017年05期

【摘要】：目的研究AIFM1基因突變對(duì)Harlequin小鼠年齡相關(guān)耳蝸聽覺功能的影響。方法用圓窗記錄耳蝸電圖來評(píng)價(jià)2月齡和6月齡野生型(WT)和AIFM1基因突變型(KO)Harlequin小鼠的耳蝸外周聽覺功能,通過Phalloi-din熒光標(biāo)記耳蝸基底膜外毛細(xì)胞的表皮板來評(píng)估毛細(xì)胞的損失程度。結(jié)果 (1)2月齡組WT型和KO型小鼠的復(fù)合動(dòng)作電位閾值分別為18.6±1.6 d B SPL(n=6)和22.2±2.2 d B SPL(n=6),兩組之間沒有統(tǒng)計(jì)學(xué)差異(t=1.282,P0.05);6個(gè)月組WT型和KO型復(fù)合動(dòng)作電位閾值分別為25.7±1.7 d B SPL(n=6)和42.1±3.6 d B SPL(n=6),兩組之間差異有統(tǒng)計(jì)學(xué)意義t=4.129,P0.01)。(2)2月齡組WT型和KO型小鼠CAP的幅值沒有統(tǒng)計(jì)學(xué)差異(P0.01),而6個(gè)月組的CAP的幅值在高頻段(30,35和40 k Hz)有統(tǒng)計(jì)學(xué)差異(Two-way ANOVA,P0.05),在低頻和中頻則沒有明顯差異。2月齡組和6月齡組WT型和KO型小鼠10 k Hz以下CM的幅值的差異沒有統(tǒng)計(jì)學(xué)意義(P0.05)。(3)耳蝸外毛細(xì)胞計(jì)數(shù)的結(jié)果顯示,2月齡組的WT型(n=5)和KO型小鼠(n=5)之間沒有統(tǒng)計(jì)學(xué)差異,而6月齡組的KO型小鼠的耳蝸外毛細(xì)胞個(gè)數(shù)在底回較WT型小鼠明顯受損(t=15.1,P0.0001)。結(jié)論 AIFM1基因突變導(dǎo)致Harlequin小鼠耳蝸基底膜底回末端外毛細(xì)胞受損,從而導(dǎo)致耳蝸外周聽覺功能高頻段受損。
[Abstract]:Objective to study the effect of AIFM1 gene mutation on age-related auditory function in Harlequin mice. Methods the cochlear peripheral auditory function of wild-type (WT) and 6-month-old Harlequin mice (WT) and AIFM1 gene mutation (KO) were evaluated by electrocochleogram recorded by round window. The loss of hair cells was evaluated by Phalloi-din fluorescent labeled epidermis of the hair cells outside the basal membrane of the cochlea. Results (1) the threshold values of complex action potential of WT and KO type mice in 2-month group were 18.6 鹵1.6 dB SPL (nong6) and 22.2 鹵2.2 dB SPL (nong6), respectively. There was no significant difference between the two groups (t 1.282 P 0.05), and the complex action potential thresholds of WT type and KO type in 6-month group were 25.7 鹵1.7 dB SPL (nong6), respectively. There was no significant difference between the two groups in the amplitude of CAP between WT and KO type mice (P0.01), but the amplitude of CAP in 6-month group had statistical difference in high frequency range (300.35 and 40kHz) (Two-way ANOVANALY P 0.05), but there was no significant difference in the amplitude of CAP between the two groups (P0.01). The difference of CAP amplitude between the two groups was significant (P0.01), but there was no significant difference in the amplitude of CAP between the two groups (P0.01), but there was no significant difference in the amplitude of CAP between the 6-month group and the 6-month group (P0.05). There was no significant difference in the amplitude of CM below 10 kHz between the WT and KO type mice of 2.2-month-old group and 6-month-old group (P0.05). (3). The results of cochlear hair cell count showed that WT type (nong5) and KO type in 2-month-old group were not significantly different from those in middle frequency group. There was no statistical difference between mice (nd5), However, the number of cochlear outer hair cells in 6-month-old KO mice was significantly damaged than that of WT mice at the bottom gyrus (tr 15.1, P 0.0001). Conclusion mutation of AIFM1 gene leads to damage of outer hair cells at the end of basal gyrus of cochlea in Harlequin mice, which results in high frequency damage of peripheral auditory function of cochlea.
【作者單位】： 暨南大學(xué)附屬珠海醫(yī)院耳鼻咽喉頭頸外科;聽力與聾病中心 紐約州立布法羅大學(xué);中國人民解放軍總醫(yī)院耳鼻咽喉頭頸外科;
【基金】：珠海市科技計(jì)劃項(xiàng)目(20171009E030055)~~
【分類號(hào)】：R764

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