本文選題：鼻息肉(NP) + 細(xì)胞外信號(hào)調(diào)節(jié)激酶2(ERK2)/磷酸化細(xì)胞外信號(hào)調(diào)節(jié)激酶2(p-ERK2)��； 參考：《山西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:1、檢測(cè)細(xì)胞外信號(hào)調(diào)節(jié)激酶2(ERK2)及磷酸化細(xì)胞外信號(hào)調(diào)節(jié)激酶2(p-ERK2)在鼻息肉(Nasal polyp,NP)組織的表達(dá)情況;2、初步探討ERK2/p-ERK2在NP發(fā)病中的臨床意義。方法:組織標(biāo)本收集于山西醫(yī)科大學(xué)第二醫(yī)院耳鼻咽喉頭頸外科行鼻內(nèi)鏡手術(shù)的慢性鼻竇炎伴鼻息肉患者(Chronic rhinosinusitis with nasal polyps,CRSwNP)(根據(jù)EPOS2012,鼻竇炎診治更新與精要)30例,起止時(shí)間為2016年6月至2016年12月期間。術(shù)中取CRSwNP患者NP標(biāo)本30份(實(shí)驗(yàn)組),同時(shí)取該患者下鼻甲正常鼻黏膜組織標(biāo)本30份(對(duì)照組)。應(yīng)用間接免疫熒光技術(shù)(Indirect immunofluorescence,IIF)檢測(cè)ERK2/p-ERK2在NP及正常鼻黏膜組織的表達(dá),并探討其臨床意義。結(jié)果:1、NP:p-ERK2主要表達(dá)在增生的上皮細(xì)胞、炎癥細(xì)胞以及腺上皮細(xì)胞,主要位于胞核;ERK2主要表達(dá)在腺上皮細(xì)胞、炎癥細(xì)胞,主要位于胞質(zhì)。正常鼻黏膜組織:p-ERK2主要表達(dá)在炎癥細(xì)胞、腺上皮細(xì)胞,主要位于胞核;ERK2主要位于炎癥細(xì)胞,主要位于胞質(zhì)。2、p-ERK2在NP和正常鼻黏膜組織的表達(dá)有統(tǒng)計(jì)學(xué)差異(P0.05);ERK2在NP和正常鼻黏膜組織的表達(dá)無統(tǒng)計(jì)學(xué)差異(P0.05)。結(jié)論:1、p-ERK2在NP的發(fā)病中可能具有重要作用,而ERK2與NP的發(fā)生可能無關(guān)。2、ERK2在NP和正常鼻黏膜組織均有表達(dá),提示ERK2可能參與鼻黏膜正常生理功能的維持。3、RAF-1-MEK-ERK2-NF-KB信號(hào)通路可能參與NP的發(fā)病。
[Abstract]:Objective to investigate the expression of extracellular signal-regulated kinase 2 (ERK2) and phosphorylated extracellular signal-regulated kinase 2 (p-ERK2) in nasal polyp (NP), and to explore the clinical significance of ERK2 / p-ERK2 in the pathogenesis of NP. Methods: tissue specimens were collected from 30 patients with chronic sinusitis associated with nasal polyps (according to EPOS2012) who underwent endoscopic sinus surgery in the Department of Otorhinolaryngology and head and neck surgery, Shanxi Medical University, and 30 patients with chronic sinusitis and nasal polyps (according to EPOS2012, update and essentials of the diagnosis and treatment of sinusitis), 30 patients with chronic sinusitis and nasal polyps were studied. The starting and ending period is from June 2016 to December 2016. Thirty NP specimens of CRSwNP patients (experimental group) and 30 specimens of normal nasal mucosa of inferior turbinate (control group) were taken during operation. The expression of ERK2 / p-ERK2 in NP and normal nasal mucosa was detected by indirect immunofluorescence technique (IIF) and its clinical significance was discussed. Results the expression of p-ERK2 was mainly in proliferative epithelial cells, inflammatory cells and glandular epithelial cells, mainly in glandular epithelial cells, inflammatory cells and cytoplasm. The normal nasal mucosa: p-ERK2 is mainly expressed in inflammatory cells, glandular epithelial cells, mainly located in nuclear ERK2, mainly in inflammatory cells. The expression of ERK2 in NP and normal nasal mucosa was significantly different (P0.05), and there was no significant difference in the expression of ERK2 between NP and normal nasal mucosa (P0.05). Conclusion 1 / 1 p-ERK2 may play an important role in the pathogenesis of NP, but ERK2 may not be related to the occurrence of NP, and ERK2 may be expressed in both NP and normal nasal mucosa. These results suggest that ERK2 may be involved in the maintenance of normal physiological function of nasal mucosa. 3RAF-1-MEK-ERK2-NF-KB signaling pathway may be involved in the pathogenesis of NP.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R765

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