發(fā)布時(shí)間：2018-07-08 09:02

  本文選題：甘蔗葉多糖 + CNE_2細(xì)胞��； 參考：《廣西醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的：研究甘蔗葉多糖對鼻咽癌CNE_2細(xì)胞株的體外抑制作用，并初步探討其作用機(jī)制。 方法:采用熱水浸提及DEAE-纖維素層析柱法提純甘蔗葉多糖，以MTT法觀察其體外抗腫瘤活性，透射電子顯微鏡觀察凋亡細(xì)胞的超微結(jié)構(gòu)，采用流式細(xì)胞術(shù)檢測腫瘤細(xì)胞凋亡率，實(shí)時(shí)熒光定量PCR法檢測凋亡相關(guān)基因Bax、Bcl-2的變化。 結(jié)果:濃度為10ug/ml、20ug/ml、40ug/ml、80ug/ml、160ug/ml的甘蔗葉多糖作用后，CNE_2細(xì)胞抑制率及凋亡率均明顯增加，且呈濃度依賴性。電鏡下可見160ug/ml濃度的甘蔗葉多糖可使CNE_2細(xì)胞出現(xiàn)凋亡小體。熒光定量PCR技術(shù)測定Bax在CNE_2細(xì)胞中表達(dá)上調(diào)，并隨著甘蔗葉多糖作用濃度的增加而升高；Bcl-2則在CNE_2細(xì)胞中表達(dá)下調(diào)，，且隨著甘蔗葉多糖作用濃度的增加而降低。 結(jié)論：甘蔗葉多糖能明顯抑制人鼻咽癌CNE_2細(xì)胞增殖，誘導(dǎo)細(xì)胞凋亡，可能通過提高Bax表達(dá)及降低Bcl-2的表達(dá)，從而發(fā)揮抗腫瘤作用。
[Abstract]:Aim: to study the inhibitory effect of sugarcane leaf polysaccharide on CNE2 cell line of nasopharyngeal carcinoma (NPC) in vitro and to explore its mechanism. Methods: the polysaccharides from sugarcane leaves were purified by hot water extraction and DEAE- cellulose chromatography. The in vitro antitumor activity was observed by MTT assay, the ultrastructure of apoptotic cells was observed by transmission electron microscope, and the apoptosis rate of tumor cells was detected by flow cytometry. The changes of apoptosis-related gene Baxon Bcl-2 were detected by real-time fluorescence quantitative PCR. Results: the inhibition rate and apoptosis rate of CNE2 cells were significantly increased after the treatment of sugar cane leaf polysaccharides at the concentration of 10ugrml / ml 20ugr / ml 40ugrml / ml ~ 80ugr / ml sugarcane leaf polysaccharide in a concentration-dependent manner. The inhibitory rate and apoptosis rate of CNE2 cells were increased in a concentration-dependent manner. Under electron microscope, 160ug/ml concentration of polysaccharides from sugarcane leaves could make CNE2 cells appear apoptotic bodies. The expression of Bax was up-regulated in CNE2 cells by fluorescence quantitative PCR, and the expression of Bcl-2 was down-regulated in CNE2 cells with the increase of polysaccharide concentration in sugarcane leaves, and decreased with the increase of polysaccharide concentration in sugarcane leaves. Conclusion: polysaccharide from sugarcane leaves can significantly inhibit the proliferation of CNE2 cells and induce apoptosis, which may play an anti-tumor role by increasing the expression of Bax and decreasing the expression of Bcl-2.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R739.63

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 位鴻,李文香,王艷英;中藥抗腫瘤作用研究進(jìn)展[J];長春中醫(yī)學(xué)院學(xué)報(bào);2001年03期

2 張小梅;黃芪多糖的免疫調(diào)節(jié)作用及抗腫瘤作用研究進(jìn)展[J];大連大學(xué)學(xué)報(bào);2003年06期

3 商澎,楊鐵虹,賈敏,梅其炳,朱德生,趙文明,趙德化;當(dāng)歸多糖AP-0對小鼠移植性腫瘤的抑制作用[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2001年11期

4 潘冉;香菇多糖臨床應(yīng)用研究進(jìn)展及香菇的藥用價(jià)值[J];海峽藥學(xué);2005年04期

5 于恩廣;王龍?jiān)?;蘆薈多糖的抗腫瘤作用[J];黑龍江醫(yī)藥;2004年02期

6 季宇彬,高世勇,成秉辰;海藻多糖抗腫瘤作用的實(shí)驗(yàn)研究[J];哈爾濱商業(yè)大學(xué)學(xué)報(bào)(自然科學(xué)版);2001年04期

7 陳 群,劉家昌;人參多糖、黃芪多糖、枸杞多糖的研究進(jìn)展[J];淮南師范學(xué)院學(xué)報(bào);2001年02期

8 劉娟妮;王雪青;魏丹;郅琪;李晨韻;龐廣昌;;螺旋藻多糖的積累與抗腫瘤作用的研究[J];海洋通報(bào);2009年06期

9 呂曉英,曾令福,蔡亞南,李由,劉新華;紅毛五加多糖-Ⅰ誘導(dǎo)體外人胃癌細(xì)胞凋亡的研究[J];華西醫(yī)科大學(xué)學(xué)報(bào);2002年02期

10 顧云燕;吳瑾瑾;;枸杞多糖抗腫瘤作用實(shí)驗(yàn)研究進(jìn)展[J];浙江臨床醫(yī)學(xué);2007年09期

相關(guān)碩士學(xué)位論文 前1條

1 李小平;紅棗多糖提取工藝研究及其生物功能初探[D];陜西師范大學(xué);2004年

本文編號：2106912