發(fā)布時(shí)間：2018-07-07 08:12

  本文選題：甲狀腺相關(guān)性眼病 + 眼眶脂肪組��； 參考：《廣西醫(yī)科大學(xué)》2012年碩士論文

【摘要】：研究目的：通過(guò)檢測(cè)miR-155和miR-146a在TAO患者眼眶脂肪組織中的表達(dá)情況,探討miR-155和miR-146a對(duì)TAO病理生理的影響及意義。 研究方法：本研究設(shè)實(shí)驗(yàn)組和對(duì)照組兩組,實(shí)驗(yàn)組眼眶脂肪組樣本來(lái)自2011-2012年間在我院行眼眶減壓術(shù)TAO患者7例,對(duì)照組眼眶脂肪組織取自因眼外傷等原因引起眼球萎縮行眼球摘除術(shù)及義眼座植入術(shù)以及Ⅱ期義眼座植入術(shù)8例。通過(guò)RT-PCR的方法(SYBR Green Ⅰ熒光染料)和甲醛變性瓊脂糖凝膠電泳的方法檢測(cè)miR-155和miR-146a在實(shí)驗(yàn)組和對(duì)照組眼眶脂肪組織中的表達(dá)情況,其檢測(cè)結(jié)果用SPSS13.0對(duì)數(shù)據(jù)進(jìn)行統(tǒng)計(jì)學(xué)分析。 結(jié)果：1.與對(duì)照組相比,miR-155在TAO組眼眶脂肪組織中的表達(dá)明顯上調(diào),是對(duì)照組的2.53倍,差異有統(tǒng)計(jì)學(xué)意義(P=0.0000.01) 2.在TAO組眼眶脂肪組織中,miR-146a比在對(duì)照組中的表達(dá)明顯下調(diào),是對(duì)照組的0.21倍,差異有統(tǒng)計(jì)學(xué)意義(P=0.0000.01) 結(jié)論：TAO患者和正常人眼眶脂肪中miR-155和miR-146a的表達(dá)存在顯著性差異,提示miR-155和miR-146a可能與TAO病程的發(fā)生發(fā)展有關(guān)。
[Abstract]:Objective: to investigate the effect of miR-155 and miR-146a on the pathophysiology of TAO by detecting the expression of miR-155 and miR-146a in orbital adipose tissue of TAO patients. Methods: this study was divided into two groups: experimental group and control group. The experimental group consisted of 7 TAO patients who underwent orbital decompression from 2011 to 2012. In the control group, orbital adipose tissue was taken from 8 cases of ocular atrophy caused by ocular trauma. The expression of miR-155 and miR-146a in orbital adipose tissue was detected by RT-PCR (SYBR Green 鈪，

本文編號(hào)：2104342