本文選題：變態(tài)反應(yīng)性鼻炎 + 嗜酸性粒細(xì)胞��； 參考：《南昌大學(xué)》2012年碩士論文

【摘要】：目的：采用體外合成的干擾RNA，特異性抑制嗜酸性粒細(xì)胞上CCR3表達(dá)，阻斷Eotaxin/CCR3路徑的作用，體內(nèi)觀察變應(yīng)性鼻炎小鼠骨髓、外周血及鼻腔灌洗液中嗜酸性粒細(xì)胞CCR3及顆粒蛋白的改變，以期了解在此過程中對嗜酸性細(xì)胞的作用及影響程度，為變應(yīng)性鼻炎尋找新的治療方法。 方法：采用鼻腔局部給藥，將體外合成的特異性CCR3siRNA重組穿梭質(zhì)粒（pLVX-ShRNA2-mCCR3-1+2+3+4）應(yīng)用于小鼠過敏性鼻炎模型上，應(yīng)用逆轉(zhuǎn)錄酶-聚合酶鏈反應(yīng)（RT-PCR）的方法分別檢測小鼠骨髓、外周血及鼻腔灌洗液中嗜酸性粒細(xì)胞CCR3及顆粒蛋白mRNA的表達(dá)水平。 結(jié)果：①在骨髓、外周血、鼻腔灌洗液中，CCR3siRNA治療組的CCR3mRNA表達(dá)低于PBS治療對照及siRNA治療對照組（P0.05）；②在骨髓、外周血、鼻腔灌洗液中，CCR3siRNA治療組的嗜酸性粒細(xì)胞顆粒蛋白--主要堿性蛋白（MBP）、陽離子蛋白（ECP）、過氧化物酶（EPO）的mRNA表達(dá)均低于PBS治療對照及siRNA治療對照組（P0.05）。 結(jié)論：在獲得高效、特異性干涉序列基礎(chǔ)上，針對基因CCR3，給予RNAi技術(shù)的靶向治療能夠抑制變應(yīng)性鼻炎動(dòng)物模型中嗜酸性粒細(xì)胞的發(fā)育、遷移及浸潤過程，從而減少嗜酸性粒細(xì)胞脫顆粒作用，，從而達(dá)到減輕變應(yīng)性鼻炎的炎癥反應(yīng)。
[Abstract]:Objective: to specifically inhibit the expression of CCR3 on eosinophils, block the eotaxin / CCR3 pathway and observe the bone marrow of allergic rhinitis mice by interference RNAs in vitro. The changes of eosinophilic granulocyte CCR3 and granulocyte in peripheral blood and nasal lavage fluid in order to understand the role and influence of eosinophil in this process and to find a new treatment for allergic rhinitis. Methods: the specific CCR3siRNA recombinant shuttle plasmid (pLVX-ShRNA2-mCCR3-1234) was used in the mouse allergic rhinitis model. The mouse bone marrow was detected by reverse transcriptase polymerase chain reaction (RT-PCR). Expression of eosinophil CCR3 and granule protein mRNA in peripheral blood and nasal lavage fluid. Results the expression of CCR3 mRNA in bone marrow, peripheral blood and nasal lavage fluid was significantly lower in CCR3siRNA group than that in PBS control group and siRNA treatment control group (P0.05). The mRNA expressions of eosinophil granulocyte protein (MBP), cationic protein (ECP) and peroxidase (EPO) in nasal lavage fluid of CCR3 siRNA treatment group were lower than those of PBS control group and siRNA treatment control group (P0.05). Conclusion: on the basis of obtaining efficient and specific interference sequences, targeted therapy with RNAi for CCR3 gene can inhibit the development, migration and infiltration of eosinophils in allergic rhinitis animal model. Thus, the eosinophil degranulation can be reduced, and the inflammatory response of allergic rhinitis can be alleviated.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R765.21

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 周明輝;董明敏;吳玉瑛;王亮;岳保紅;;Eotaxin基因和趨化因子受體3在變應(yīng)性鼻炎大鼠模型鼻腔黏膜和骨髓中的表達(dá)及意義[J];臨床耳鼻咽喉科雜志;2006年05期

本文編號(hào)：2078608