本文選題：喉癌 + 喉角化癥��； 參考：《安徽醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的 喉鱗狀細(xì)胞癌是指發(fā)生于喉黏膜上皮的惡性腫瘤，是耳鼻咽喉頭頸外科最常見的惡性腫瘤之一。喉癌抑癌基因RASSF1A的異常表達(dá)與喉鱗狀細(xì)胞癌的關(guān)系近年來國內(nèi)鮮有報(bào)道。RASSF1A基因通過干預(yù)細(xì)胞周期、抑制細(xì)胞生長、促進(jìn)細(xì)胞凋亡而抑制腫瘤的發(fā)生。該基因啟動(dòng)子發(fā)生甲基化之后會(huì)引起基因的失活，導(dǎo)致細(xì)胞周期紊亂而發(fā)生癌變。本研究觀察喉鱗狀細(xì)胞癌組織中的RASSF1A表達(dá)情況，探討RASSF1A基因的表達(dá)與喉鱗狀細(xì)胞癌的發(fā)生及病變進(jìn)展的關(guān)系，從而有望在臨床診療中使其成為一項(xiàng)新的診斷及預(yù)后評(píng)估的指標(biāo)，以提高早期診斷率及腫瘤的治愈率。 喉角化癥是喉的癌前病變,是喉部黏膜上皮生長、成熟異常以及黏膜上皮過度角化的一種增生性疾病。RASSF1A基因在喉的癌前病變組織中的甲基化及表達(dá)方面研究甚少。本研究觀察喉角化癥組織中RASSF1A的表達(dá)情況，來初步探討RASSF1A基因與喉角化癥發(fā)病是否具有相關(guān)性。 方法 收集安徽省立耳鼻咽喉頭頸外科自2009-2011年的術(shù)中切除的聲帶息肉、喉角化癥及喉鱗狀細(xì)胞癌組織，另外取尸頭正常聲帶黏膜。用免疫組織化學(xué)方法檢測各組組織的RASSF1A蛋白表達(dá)情況。RASSF1A蛋白經(jīng)免疫組織化學(xué)方法染色后為棕黃色顆粒，在細(xì)胞漿內(nèi)彌漫性分布，細(xì)胞核不著色。計(jì)算每例組織切片中RASSF1A陽性細(xì)胞百分?jǐn)?shù)，取平均值±標(biāo)準(zhǔn)差(x±s)，采用SPSS17.0統(tǒng)計(jì)分析軟件對(duì)數(shù)據(jù)進(jìn)行分析，P＜0.05為差異有統(tǒng)計(jì)學(xué)意義。 結(jié)果 RASSF1A在喉鱗狀細(xì)胞癌及喉角化癥組織中的表達(dá)低于聲帶息肉組織和正常喉黏膜組織；喉鱗狀細(xì)胞癌與聲帶息肉，喉鱗狀細(xì)胞癌與正常喉黏膜及喉鱗狀細(xì)胞癌與喉角化癥進(jìn)行比較，RASSF1A表達(dá)差異均具有統(tǒng)計(jì)學(xué)意義(P0.05)。 喉角化癥與聲帶息肉比較，喉角化癥與正常喉黏膜組織比較以及喉角化癥各類型之間進(jìn)行比較，RASSF1A表達(dá)差異不具有統(tǒng)計(jì)學(xué)意義(P0.05)。 RASSF1A在喉鱗狀細(xì)胞癌臨床I-IV期中的表達(dá)情況呈現(xiàn)逐漸下降趨勢，各臨床分期間差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。RASSF1A在高分化、中分化及低分化癌組織中表達(dá)差異以及各年齡組之間表達(dá)的差異亦不具有統(tǒng)計(jì)學(xué)意(P0.05)。 結(jié)論 1.喉鱗狀細(xì)胞癌組織中RASSFlA蛋白的表達(dá)程度明顯低于正常喉組織及聲帶息肉組織，提示RASSFlA蛋白表達(dá)降低與喉鱗狀細(xì)胞癌的發(fā)生有關(guān); 2.RASSFlA的表達(dá)與患者年齡及組織的病理學(xué)分化程度無關(guān)，但與喉鱗狀細(xì)胞癌的臨床分期有關(guān)，不同臨床分期喉癌組織RASSFlA蛋白的表達(dá)程度不同，臨床分期越早，RASSFlA蛋白表達(dá)水平越高;臨床分期越晚，RASSFlA蛋白表達(dá)水平越低；提示RASSFlA的表達(dá)可能與腫瘤的浸潤及轉(zhuǎn)移有關(guān)。 3.RASSF1A基因可能參與了喉鱗狀細(xì)胞癌發(fā)生的早期過程，因此可以利用此特征作為喉鱗狀細(xì)胞癌的早期診斷指標(biāo)，并且對(duì)于在鑒別癌前病變與喉癌時(shí)可能具有一定意義; 4.對(duì)RASSFlA蛋白表達(dá)進(jìn)行檢測，有助于篩查RASSFlA基因異常的人群，，提搞喉鱗狀細(xì)胞癌的早期診斷水平。
[Abstract]:Purpose

The expression of RASSF1A gene in laryngeal squamous cell carcinoma and its relationship with laryngeal squamous cell carcinoma have been reported .

RASSF1A gene is a kind of proliferative disease in laryngeal carcinoma . The RASSF1A gene has been used to investigate the methylation and expression of RASSF1A gene in laryngeal carcinoma .

method

The expression of RASSF1A protein was detected by immunohistochemical method . RASSF1A protein was stained by immunohistochemistry . The percentage of RASSF1A positive cells in each group was calculated and the mean 鹵 standard deviation ( x 鹵 s ) was calculated . The data were analyzed by SPSS 17.0 statistical analysis software , and the difference was statistically significant .

Results

The expression of RASSF1A in laryngeal squamous cell carcinoma and laryngeal keratopathy was lower than that of vocal cord polyp and normal laryngeal mucosa .
The expression of RASSF1A in laryngeal squamous cell carcinoma and vocal cord polyp , laryngeal squamous cell carcinoma and normal laryngeal mucosa and laryngeal squamous cell carcinoma were statistically significant ( P0.05 ) .

There was no significant difference in RASSF1A expression compared with vocal cord polyp ( P0.05 ) .

RASSF1A showed a decreasing trend in the clinical I - IV period of laryngeal squamous cell carcinoma ( P0.05 ) . RASSF1A had no statistical significance in the expression of RASSF1A in high differentiation , middle differentiation and low differentiated cancer tissues and the difference between different age groups ( P0.05 ) .

Conclusion

1 . The expression of RASSFlA protein in laryngeal squamous cell carcinoma was significantly lower than that in normal laryngeal tissues and vocal cord polyp tissues , suggesting that RASSFlA protein expression was associated with the occurrence of laryngeal squamous cell carcinoma ;

2 . The expression of RASSFlA was not related to the age of the patient and the pathological differentiation degree of the tissues , but the expression level of RASSFlA protein was different from the clinical stage of laryngeal squamous cell carcinoma . The higher the expression level of RASSFlA protein in different clinical stages , the higher the expression level of RASSFlA protein , the lower the level of RASSFlA protein expression was .
It is suggested that the expression of RASSFlA may be related to invasion and metastasis of tumor .

3 . RASSF1A gene may be involved in the early stage of laryngeal squamous cell carcinoma , so it can be used as an early diagnostic index for laryngeal squamous cell carcinoma , and may be of certain significance in identifying precancerous lesions and laryngeal cancer ;

4 . The expression of RASSFlA protein is detected , which helps to screen the abnormal population of RASSFlA gene , which can be used for early diagnosis of laryngeal squamous cell carcinoma .
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R739.65

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 許承弼;滕博;李長青;金春順;;喉鱗狀細(xì)胞癌中RASSF1A基因啟動(dòng)子區(qū)甲基化及蛋白表達(dá)[J];吉林大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2006年02期

2 郭偉;黃志剛;陳曉紅;范爾鐘;許洪波;;喉癌及癌前病變中細(xì)胞周期調(diào)控基因甲基化的研究[J];中國耳鼻咽喉頭頸外科;2009年03期

3 向元O

本文編號(hào)：2073156