本文選題：年齡相關性黃斑變性 + 脈絡膜新生血管�。� 參考：《眼科新進展》2017年10期

【摘要】：目的分別從體外實驗和體內(nèi)實驗兩個方面探討人參皂苷RG3對脈絡膜新生血管的抑制作用。方法體外實驗:MTT檢測人參皂苷RG3對人臍靜脈內(nèi)皮細胞(human umbilical vein endothelial cells,HUVEC)增殖的抑制作用,HUVEC分為正常組(含體積分數(shù)10%胎牛血清的DMEM/F~(-1)2培養(yǎng)基)、對照組(含2 g·L~(-1)DMSO)和12.5μmol·L~(-1)、25.0μmol·L~(-1)、50.0μmol·L~(-1)、100.0μmol·L~(-1)人參皂苷RG3給藥組,給藥處理6 h后測定其吸光度值。HUVEC分為對照組(含2 g·L~(-1)DMSO)和100.0μmol·L~(-1)人參皂苷RG3組,小管形成實驗檢測人參皂苷RG3對小管形成的抑制作用,Western blotting檢測人參皂苷RG3對VEGF蛋白表達的影響。體內(nèi)實驗:雄性C57BL/6J小鼠20只,隨機分為對照小鼠組和人參皂苷RG3小鼠組,皮下注射預處理給藥2周,半導體激光造模,于光凝后第1天和第21天行熒光素眼底血管造影檢查。結(jié)果 MTT結(jié)果顯示,正常組、對照組、12.5μmol·L~(-1)、25.0μmol·L~(-1)、50.0μmol·L~(-1)、100.0μmol·L~(-1)人參皂苷RG3給藥組的吸光度值分別為0.43±0.17、0.43±0.05、0.33±0.02、0.24±0.02、0.18±0.01、0.15±0.01,對照組與正常組相比差異無統(tǒng)計學意義(P0.05),其余各組與對照組相比差異均有統(tǒng)計學意義(均為P0.05)。小管形成實驗結(jié)果顯示,對照組與100.0μmol·L~(-1)人參皂苷RG3組小管形成數(shù)目分別為(72.5±5.56)個和(11.33±3.71)個,差異有統(tǒng)計學意義(P0.01)。Western blotting結(jié)果顯示,100.0μmol·L~(-1)人參皂苷RG3組的VEGF蛋白相對表達量(0.14±0.01)明顯低于對照組(0.46±0.01),差異有統(tǒng)計學意義(P0.01)。體內(nèi)實驗熒光素眼底血管造影結(jié)果顯示,人參皂苷RG3小鼠組的熒光滲漏面積明顯低于對照小鼠組。結(jié)論人參皂苷RG3可以抑制脈絡膜新生血管的形成。
[Abstract]:Objective to investigate the inhibitory effect of ginsenoside RG3 on choroidal neovascularization in vitro and in vivo. Methods the inhibitory effect of ginsenoside RG3 on the proliferation of human umbilical vein endothelial cells (HUVECs) in vitro was assayed. RG3 was divided into three groups: normal group (DMEM/F~(-1)2 medium containing 10% fetal bovine serum), control group (containing 2 g / L) and 12.5 渭 mol / L ~ (25. 0) 渭 mol / L ~ (-1) ~ (50.0) 渭 mol / L ~ (10) mol / L ~ (-1) ginsenoside RG3 group. After 6 hours of administration, the absorbency value of ginsenoside RG3 was measured. HUVECs were divided into two groups: control group (containing 2 g mol) and ginsenoside RG3 group (100.0 渭 mol / L). The inhibition of ginsenoside RG3 on tubule formation was detected by tubulogenesis assay and the effect of ginsenoside RG3 on VEGF protein expression was detected by Western blotting. In vivo experiment: 20 male C57BL/6J mice were randomly divided into two groups: control group and ginsenoside RG3 group. The mice were pretreated by subcutaneous injection for 2 weeks, then the model was made by semiconductor laser, and the fundus fluorescein angiography was performed on the 1st and 21st day after photocoagulation. Results the results of MTT showed that the absorbance values of ginsenoside RG3 group were 0.43 鹵0.170.43 鹵0.050.33 鹵0.020.24 鹵0.02U 0.18 鹵0.01U 鹵0.15 鹵0.01.There was no significant difference between the control group and the control group (P 0.05), and there was no significant difference between the control group and the normal group (P 0.05). The absorbance of ginsenoside RG3 group was 0.43 鹵0.170.43 鹵0.050.33 鹵0.020.24 鹵0.02U 0.18 鹵0.01 鹵0.15 鹵0.01.There was no significant difference between the control group and the normal group (P 0.05). The results of tubulogenesis experiment showed that the number of tubules in control group and 100.0 渭 mol RG3 group was 72.5 鹵5.56 and 11.33 鹵3.71, respectively. The relative expression of VEGF protein in the ginsenoside RG3 group (0.14 鹵0.01) was significantly lower than that in the control group (0.46 鹵0.01), and the difference was statistically significant (P 0.01). In vivo fluorescein fundus angiography showed that the fluorescence leakage area of ginsenoside RG3 mice group was significantly lower than that of control group. Conclusion ginsenoside RG3 can inhibit choroidal neovascularization.
【作者單位】： 錦州醫(yī)科大學;
【基金】：遼寧省科學技術基金[面上項目(原優(yōu)秀人才培育)](編號:201602298) 遼寧省科技廳-錦州醫(yī)科大學聯(lián)合基金項目(編號:20170540382)~~
【分類號】：R774.5

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