本文選題：視網(wǎng)膜脫離 + 腦源性神經(jīng)營(yíng)養(yǎng)因子��； 參考：《華中科技大學(xué)》2012年碩士論文

【摘要】：目的通過建立大鼠眼視網(wǎng)膜脫離（Retinal detachment，RD）模型，觀察RD復(fù)位后采用向玻璃體腔注入腦源性神經(jīng)營(yíng)養(yǎng)因子（Brain-derived neurotrophicfactor， BDNF）的給藥方式，從光感受器細(xì)胞的凋亡，RD復(fù)位后視網(wǎng)膜形態(tài)學(xué)和視網(wǎng)膜細(xì)胞超微結(jié)構(gòu)方面探討B(tài)DNF對(duì)RD損傷后視網(wǎng)膜修復(fù)的影響，為采用神經(jīng)營(yíng)養(yǎng)因子抑制RD復(fù)位后細(xì)胞凋亡，促進(jìn)視網(wǎng)膜功能恢復(fù)提供理論依據(jù)。 方法 1.取健康SD大鼠56只，通過在視網(wǎng)膜下注射透明質(zhì)酸鈉的方法建立RD模型，并觀察視網(wǎng)膜自動(dòng)復(fù)位的時(shí)間。 2.將56只大鼠隨機(jī)分為四組，BDNF實(shí)驗(yàn)組、實(shí)驗(yàn)對(duì)照組、RD復(fù)位組和正常對(duì)照組，BDNF實(shí)驗(yàn)組、實(shí)驗(yàn)對(duì)照組在視網(wǎng)膜自動(dòng)復(fù)位的當(dāng)日分別在玻璃體腔注射BDNF（20μg/ml）10μl和生理鹽水（normal saline， NS）10μl。 3.采用光鏡和電鏡觀察RD復(fù)位后視網(wǎng)膜形態(tài)和視網(wǎng)膜細(xì)胞超微結(jié)構(gòu)，檢測(cè)BDNF對(duì)RD造成的視網(wǎng)膜變性的影響；采用原位缺口末端原位末端轉(zhuǎn)移酶標(biāo)記法（Terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphatenick-end labeling，TUNEL）檢測(cè)BDNF對(duì)RD復(fù)位后視網(wǎng)膜細(xì)胞凋亡的影響。 結(jié)果 1.視網(wǎng)膜自動(dòng)復(fù)位的平均時(shí)間為31.3±3.5天。 2.光鏡觀察：與正常對(duì)照組相比，RD復(fù)位后視網(wǎng)膜整體厚度變薄，內(nèi)核層，內(nèi)外核層厚度變薄，尤以外核層明顯，視錐視桿細(xì)胞層細(xì)胞稀疏，排列紊亂，外從狀層明顯變��；神經(jīng)節(jié)細(xì)胞層細(xì)胞數(shù)目變少，神經(jīng)纖維層變薄。與實(shí)驗(yàn)對(duì)照組相比，BDNF實(shí)驗(yàn)組視網(wǎng)膜整體厚度較實(shí)驗(yàn)對(duì)照組厚，內(nèi)外核層皆較厚，尤以外核層明顯，光感受器細(xì)胞層排列更整齊，細(xì)胞密度較大，神經(jīng)節(jié)細(xì)胞層細(xì)胞數(shù)目較多，排列較好。視網(wǎng)膜外核層厚度比較：正常組視網(wǎng)膜外核層厚度平均為39.446μm，與其余各組比較有統(tǒng)計(jì)學(xué)意義（P＜0.05）；BDNF實(shí)驗(yàn)組平均厚度為23.509μm，實(shí)驗(yàn)對(duì)照組平均厚度為13.496μm，BDNF實(shí)驗(yàn)組與實(shí)驗(yàn)對(duì)照組相比較有顯著性差異（P＜0.01）。視網(wǎng)膜內(nèi)核層厚度比較：正常組視網(wǎng)膜內(nèi)核層厚度平均為24.636μm，與其余各組相比有統(tǒng)計(jì)學(xué)意義（P＜0.05）；BDNF實(shí)驗(yàn)組平均厚度為21.166μm，實(shí)驗(yàn)對(duì)照組平均厚度為16.084μm，BDNF實(shí)驗(yàn)組與實(shí)驗(yàn)對(duì)照組相比較有顯著性差異（P＜0.01）。 3.電鏡觀察：與正常對(duì)照組相比，RD復(fù)位組視網(wǎng)膜各層損害都較嚴(yán)重，各層結(jié)構(gòu)排列紊亂，空泡樣變，內(nèi)核層細(xì)胞核周間隙變大，核染色質(zhì)疏松，核有溶解；神經(jīng)節(jié)細(xì)胞核染色質(zhì)疏松，纖維層神經(jīng)節(jié)細(xì)胞軸突腫脹。與實(shí)驗(yàn)對(duì)照組相比，BDNF實(shí)驗(yàn)組內(nèi)外節(jié)損害較輕、視細(xì)胞數(shù)較多、外核層較厚、視網(wǎng)膜結(jié)構(gòu)和層次排列較好，視網(wǎng)膜各層變性較輕。 4. TUNEL觀察：除正常對(duì)照組外，，BDNF實(shí)驗(yàn)組，實(shí)驗(yàn)對(duì)照組，RD復(fù)位組都能見到TUNEL陽性細(xì)胞，主要在視網(wǎng)膜內(nèi)外核層，感光細(xì)胞層間也可見。實(shí)驗(yàn)對(duì)照組和RD復(fù)位對(duì)照組內(nèi)外核層和光感受器細(xì)胞層TUNEL陽性細(xì)胞清晰可數(shù)，而BDNF實(shí)驗(yàn)組內(nèi)外核層及感光細(xì)胞層間TUNEL陽性細(xì)胞較實(shí)驗(yàn)對(duì)照組少。 結(jié)論 1.RD復(fù)位后視網(wǎng)膜形態(tài)及視網(wǎng)膜細(xì)胞超微結(jié)構(gòu)發(fā)生了改變。 2.RD復(fù)位后視網(wǎng)膜的視細(xì)胞有凋亡的存在。 3.外源性BDNF能抑制RD復(fù)位后視網(wǎng)膜細(xì)胞的凋亡，對(duì)視網(wǎng)膜脫離復(fù)位后視細(xì)胞損傷有一定的保護(hù)作用，為采用神經(jīng)營(yíng)養(yǎng)因子促進(jìn)視網(wǎng)膜功能恢復(fù)提供新的理論依據(jù)。
[Abstract]:Objective To establish a model of retinal detachment ( RD ) in rats , and observe the effect of BDNF on retinal repair after RD reduction by injecting brain - derived neurotrophic factor ( BDNF ) into vitreous cavity after RD reduction .

method

1 . Using 56 healthy SD rats , the RD model was established by injecting sodium hyaluronate under the retina , and the time of auto - reduction of the retina was observed .

2 . Fifty - six rats were randomly divided into four groups : BDNF experimental group , experimental control group , RD reduction group and normal control group , BDNF group and experimental control group . BDNF ( 20 渭g / ml ) 10 渭l and normal saline ( NS ) 10 渭l were injected into vitreous cavity on the day of auto - reduction of retina .

3 . The retinal morphology and ultrastructure of retinal cells were observed by light microscope and electron microscope . The effects of BDNF on retinal degeneration caused by RD were detected .
閲囩敤鍘熶綅緙哄彛鏈鍘熶綅鏈杞Щ閰舵爣璁版硶(Terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphatenick-end labeling,TUNEL)媯

本文編號(hào)：1958049