本文選題：中樞聽覺處理障礙 + CN�。� 參考：《南京醫(yī)科大學學報(自然科學版)》2017年05期

【摘要】：目的 :采用同位素相對標記和絕對定量(isobaric tags for relative and absolute quantitation,iTRAQi)技術(shù)分析耳蝸核(cochlear nucleus,CN)區(qū)域特異性膜蛋白。方法 :出生60 d的雄性SD大鼠分為4組,分別是CN、下丘(inferior colliculus,IC)、上橄欖核(superior oliver complex,SOC)、大腦其余部位(Rest)。提取這4個部位組織的細胞膜蛋白,再采用iTRAQi技術(shù)檢測出CN區(qū)域特異性蛋白。通過Uniprot檢索這些蛋白的功能和參與的生物代謝過程。結(jié)果:本實驗最終得到17種CN區(qū)域特異性膜蛋白。對CN區(qū)域蛋白進行基因本體(gene ontology,GO)分析發(fā)現(xiàn),這些蛋白主要參與生物調(diào)節(jié)與發(fā)育。UNIPROT檢索發(fā)現(xiàn)3種蛋白與神經(jīng)系統(tǒng)功能相關(guān),即半乳糖凝集素-1(galectin-1,Gal-1)、膜聯(lián)蛋白A2(annexin-A2,AXNA2)和外周蛋白(peripherin,PRPH)。Gal-1與神經(jīng)生長發(fā)育相關(guān);AXNA2參與多種生物調(diào)節(jié)過程;PRPH與神經(jīng)退行性病變相關(guān)。其中,Gal-1可促進軸突生長、調(diào)節(jié)突觸可塑性且具有神經(jīng)保護作用;AXNA2可能參與了神經(jīng)遞質(zhì)釋放過程,且有神經(jīng)營養(yǎng)作用;PRPH在高表達時具有神經(jīng)毒性,和神經(jīng)絲蛋白共同參與神經(jīng)退行性病變。結(jié)論:Gal-1、ANXA2和PRPH在耳蝸核中高度表達,可能與中樞信息處理相關(guān),這為進一步研究中樞聽覺處理障礙發(fā)病機制提供了一定的理論基礎(chǔ)。
[Abstract]:Objective: to analyze cochlear nucleus specific membrane protein by using isotopic relative labeling and absolute quantitative tags for relative and absolute quantitative analysis. Methods: male Sprague-Dawley rats (60 days old) were divided into 4 groups: CNS, inferior colliculus, superior olivary nucleus superior oliver complex oliver, and rest of brain. Cell membrane proteins were extracted from the four tissues and CN region-specific proteins were detected by iTRAQi. The function of these proteins and their involvement in biological metabolic processes were retrieved by Uniprot. Results: 17 CN domain specific membrane proteins were obtained. The analysis of CN domain proteins revealed that these proteins were mainly involved in biological regulation and development. UNIPROT search revealed that three proteins were related to nervous system function. That is, galactose-galectin-1 (Gal-1), annexin-A2annexin-A2AXNA2) and peripheral protein (PRPH.Gal-1) are involved in various biological regulation processes, PRPH and neurodegenerative diseases. Gal-1 can promote axon growth, regulate synaptic plasticity and have neuroprotective effect. AXNA2 may be involved in the release of neurotransmitters. And neurofilament proteins are involved in neurodegenerative lesions. Conclusion the high expression of ANXA2 and PRPH in the cochlear nucleus may be related to the central information processing, which provides a theoretical basis for the further study of the pathogenesis of central auditory processing disorders.
【作者單位】： 南京醫(yī)科大學第一附屬醫(yī)院耳鼻咽喉科WHO預防聾和聽力減退合作中心;
【基金】：[基金項目]江蘇省“科教興衛(wèi)工程”醫(yī)學重點人才(RC2011073)
【分類號】：R764.4
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本文編號：1952616