發(fā)布時(shí)間：2018-05-28 16:04

  本文選題：拉曼光譜 + 鼻咽癌��； 參考：《福建師范大學(xué)》2012年博士論文

【摘要】：鼻咽癌(Nasopharyngeal Carcinoma, NPC)是危害人類健康和生命的頭頸部癌癥之一,其發(fā)病率居耳鼻喉科惡性腫瘤之首。長期以來鼻咽癌診斷漏診率較高,早期特別是Ⅰ期診斷率不高,而早期診斷后進(jìn)行有效治療是提高鼻咽癌患者生存率和降低死亡率的關(guān)鍵因素之一。本文利用拉曼光譜技術(shù)針對鼻咽癌組織、鼻咽正常組織及三種不同種類的鼻咽癌細(xì)胞開展一系列的拉曼光譜測量與分析,探討鼻咽組織癌變過程中生化物質(zhì)組成的變化,進(jìn)而尋求實(shí)現(xiàn)鼻咽癌早期診斷的新方法,并在此基礎(chǔ)上結(jié)合當(dāng)今技術(shù)發(fā)展及臨床需求,開展活體生物組織拉曼光譜測量裝置的研究。 本文首先簡要介紹了拉曼光譜的基本原理；綜述了拉曼光譜技術(shù)在生物醫(yī)學(xué)中的研究進(jìn)展,并針對拉曼光譜數(shù)據(jù)處理方法進(jìn)行了較為詳細(xì)的分析與討論。 其次,利用顯微拉曼光譜儀及一套自行研制的快速拉曼光譜檢測系統(tǒng)針對鼻咽癌組織、鼻咽正常組織及鼻咽癌細(xì)胞開展拉曼光譜測量與分析,分析鼻咽組織癌變過程中生化物質(zhì)組成的變化,并主要通過主成分分析和判別分析構(gòu)建判別算法,對鼻咽癌變組織與鼻咽正常組織以及三種不同種類的細(xì)胞進(jìn)行判別,探討正常組織與癌變組織以及不同細(xì)胞間的差別。 最后,利用一臺(tái)半導(dǎo)體激光器、光柵光譜儀、CCD探測器及鼻咽內(nèi)窺鏡,設(shè)計(jì)、組裝了一套可能應(yīng)用于生物組織活體測量的拉曼光譜快速檢測系統(tǒng),并在小鼠模型上進(jìn)行嘗試。初步結(jié)果表明,該系統(tǒng)可在短時(shí)間內(nèi)(3-5s)獲得活體組織的高質(zhì)量拉曼光譜信號(hào),具有較高的應(yīng)用潛力。 研究結(jié)果表明,拉曼光譜技術(shù)在區(qū)分鼻咽正常組織和鼻咽癌變組織以及不同種類的鼻咽細(xì)胞等方面具有巨大的潛力,所構(gòu)建的快速拉曼光譜檢測系統(tǒng)可用于活體組織測量。本文研究結(jié)果將為拉曼光譜在鼻咽癌早期檢測進(jìn)而拓展拉曼光譜在生物醫(yī)學(xué)中的應(yīng)用提供重要的實(shí)驗(yàn)和理論依據(jù)。
[Abstract]:Nasopharyngeal carcinoma (NPC) is one of the head and neck cancers that endanger human health and life. For a long time, the rate of missed diagnosis of nasopharyngeal carcinoma is high, but the rate of early diagnosis, especially stage I diagnosis is not high, and effective treatment after early diagnosis is one of the key factors to improve the survival rate and reduce the mortality rate of nasopharyngeal carcinoma patients. In this paper, a series of Raman spectroscopy was used to measure and analyze nasopharyngeal carcinoma (NPC) tissues, nasopharyngeal normal tissues and three kinds of nasopharyngeal carcinoma cells, and to investigate the changes of biochemical composition during nasopharyngeal carcinogenesis. Furthermore, a new method for early diagnosis of nasopharyngeal carcinoma is sought. Based on this, the research of Raman spectrometer for living tissue is carried out in combination with the development of current technology and clinical demand. In this paper, the basic principle of Raman spectroscopy is briefly introduced, the research progress of Raman spectroscopy in biomedicine is reviewed, and the methods of Raman spectrum data processing are analyzed and discussed in detail. Secondly, Raman spectroscopy was used to measure and analyze nasopharyngeal carcinoma tissue, nasopharyngeal normal tissue and nasopharyngeal carcinoma cells by using micro-Raman spectrometer and a set of self-developed rapid Raman spectrum detection system. The changes of biochemical composition in nasopharyngeal carcinogenesis were analyzed, and the discriminant algorithm was constructed by principal component analysis and discriminant analysis to distinguish nasopharyngeal carcinoma from normal nasopharynx and three different types of cells. To explore the difference between normal tissue and cancerous tissue and different cells. Finally, using a semiconductor laser, a grating spectrometer, a CCD detector and a nasopharyngeal endoscope, a rapid Raman spectrum detection system is designed and assembled, which may be applied to the in vivo measurement of biological tissue. The preliminary results show that the system can obtain high quality Raman spectra of living tissues in a short time, and has a high potential for application. The results show that Raman spectroscopy has great potential in distinguishing nasopharyngeal normal tissues from nasopharyngeal cancerous tissues and different types of nasopharyngeal cells. The results of this study will provide an important experimental and theoretical basis for the early detection of nasopharyngeal carcinoma and the application of Raman spectroscopy in biomedicine.
【學(xué)位授予單位】：福建師范大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2012
【分類號(hào)】：R739.63

【引證文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 盧玉棟;纖維素模板法制備SERS基底及在鼻咽癌診斷中的應(yīng)用[D];福建農(nóng)林大學(xué);2014年

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本文編號(hào)：1947339