本文選題：毛細(xì)胞 + 突觸帶��； 參考：《中華耳科學(xué)雜志》2017年02期

【摘要】：目的了解C57小鼠耳蝸毛細(xì)胞突觸帶發(fā)育的時間特點(diǎn)。方法全耳蝸基底膜鋪片后免疫熒光染色,共聚焦顯微鏡觀察和記錄不同發(fā)育階段C57小鼠聽覺突觸前膜突觸帶的數(shù)目與分布。運(yùn)用ABR檢測聽力發(fā)生的時間。結(jié)果 C57小鼠出生后0d,在內(nèi)外毛細(xì)胞胞質(zhì)即可觀察到Ctbp2信號;出生后第3天,Ctbp2信號在毛細(xì)胞上逐漸增多;出生后第6天,Ctbp2信號在毛細(xì)胞的胞質(zhì)和基底側(cè)膜上的表達(dá)達(dá)高峰,內(nèi)毛細(xì)胞(35.1±3.4)個/細(xì)胞,而外毛細(xì)胞上的增加更為顯著,達(dá)(21.0±3.2)個/細(xì)胞;出生后第12、30和60天,Ctbp2信號數(shù)目趨于穩(wěn)定,在內(nèi)毛細(xì)胞16-20個/細(xì)胞,外毛細(xì)胞1-3個/細(xì)胞,信號的分布也逐漸聚集在毛細(xì)胞的基底側(cè)膜上。ABR檢測提示C57小鼠聽覺發(fā)生的平均時間為13.2天。結(jié)論毛細(xì)胞突觸帶出生時即已存在,出生后逐漸發(fā)育和完善。聽覺發(fā)生與突觸帶成熟時間上的高度吻合,表明成熟突觸帶的形成可能是聽覺發(fā)生的前提條件之一。
[Abstract]:Objective to investigate the temporal characteristics of synaptic band development in cochlear hair cells of C 57 mice. Methods the number and distribution of auditory presynaptic synaptic bands in C57 mice were observed and recorded by confocal microscope after the preparation of the basal membrane of the whole cochlea. The time of hearing was measured by ABR. Results the Ctbp2 signal was observed in the cytoplasm of the inner and outer hair cells of C57 mice at 0 d after birth, the Ctbp2 signal increased gradually on the third day after birth, and the expression of Ctbp2 signal reached its peak on the cytoplasm and basal lateral membrane of the hair cells on the 6th day after birth. The number of Ctbp2 signals in the inner hair cells increased from 35.1 鹵3.4 to 21.0 鹵3.2 per cell, and the number of Ctbp2 signals on the 30th and 60th day after birth tended to be stable, with 16-20 / cells in the inner hair cells and 1-3 / cell in the outer hair cells. The signal distribution also gradually gathered on the basal membrane of hair cells. ABR detection showed that the average auditory time of C57 mice was 13.2 days. Conclusion the hair cell synaptic band existed at birth and developed and improved gradually after birth. It is suggested that the formation of mature synaptic bands may be one of the preconditions for auditory development.
【作者單位】： 南方醫(yī)科大學(xué);廣東省第二人民醫(yī)院耳鼻咽喉頭頸外科;首都醫(yī)科大學(xué)附屬友誼醫(yī)院耳鼻咽喉頭頸外科;中國人民解放軍總醫(yī)院耳鼻咽喉頭頸外科;
【基金】：國家自然科學(xué)基金(81572666)~~
【分類號】：R764

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