本文選題：糖尿病視網(wǎng)膜病變 + 玻璃體腔注射　； 參考：《重慶醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的： 從視網(wǎng)膜功能及結(jié)構(gòu)兩方面驗(yàn)證：早期糖尿病（DM）大鼠即出現(xiàn)視網(wǎng)膜神經(jīng)損害。探討玻璃體腔注射環(huán)孢素A（CsA）對(duì)早期DM大鼠視網(wǎng)膜電流圖（ERG）及視網(wǎng)膜組織結(jié)構(gòu)、超微結(jié)構(gòu)的影響。 方法： 健康雄性SD大鼠經(jīng)鏈脲佐菌素誘導(dǎo)建立DM動(dòng)物模型。 實(shí)驗(yàn)分為正常對(duì)照組、未注射藥物組（DM組）、注射CsA干預(yù)組（DM+CsA組）、注射對(duì)照液干預(yù)組（DM+DMSO組），每組9只。 于建模成功后4周進(jìn)行玻璃體腔注射，注射后48h應(yīng)用ERG檢測(cè)視網(wǎng)膜功能。然后處死大鼠獲取視網(wǎng)膜，制備視網(wǎng)膜HE染色切片及超薄切片，，應(yīng)用光鏡、透射電鏡檢測(cè)視網(wǎng)膜結(jié)構(gòu)變化。 采用GraphPad Prism5統(tǒng)計(jì)學(xué)軟件進(jìn)行統(tǒng)計(jì)分析。應(yīng)用采用單因素方差分析ERG結(jié)果中a波、b波的振幅及潛伏期數(shù)據(jù)。 結(jié)果： （1）與正常對(duì)照組相比，3組進(jìn)行不同干預(yù)的DM大鼠均有： ①ERG檢測(cè)發(fā)現(xiàn)a波、b波振幅下降； ②視網(wǎng)膜切片HE染色光鏡觀察發(fā)現(xiàn)視網(wǎng)膜外核層疏松變�。� ③視網(wǎng)膜超薄切片透射電鏡觀察發(fā)現(xiàn)細(xì)胞超微結(jié)構(gòu)紊亂呈凋亡表現(xiàn)。 （2）與DM組及DM+DMSO組相比，DM+CsA組表現(xiàn)為： ①ERG a波、b波振幅下降程度輕（P值均＜0.05）； ②外核層疏松變薄程度輕； ③超微結(jié)構(gòu)改變程度輕。 結(jié)論： DM大鼠病程早期即出現(xiàn)視網(wǎng)膜神經(jīng)功能的損害，并有視網(wǎng)膜組織結(jié)構(gòu)紊亂、神經(jīng)細(xì)胞超微結(jié)構(gòu)改變。 玻璃體腔內(nèi)注射CsA可對(duì)糖尿病視網(wǎng)膜功能及結(jié)構(gòu)起保護(hù)作用。
[Abstract]:Objective: The retinal nerve damage occurred in early diabetic rats. To investigate the effects of intravitreal injection of cyclosporine (CSA) on ERG) and the structure and ultrastructure of retina in early diabetic rats. Methods: DM model was induced by streptozotocin in healthy male SD rats. The experiment was divided into normal control group, non-injection group, CsA intervention group, DM CsA group, and control group, 9 rats in each group. Vitreous injection was performed 4 weeks after successful modeling. ERG was used to detect retinal function 48 hours after injection. Then the rats were sacrificed to obtain the retina and to prepare the HE stained and ultrathin sections of the retina. The structure of the retina was examined by light microscope and transmission electron microscope. GraphPad Prism5 statistical software was used for statistical analysis. The amplitudes and latencies of a wave / b wave in ERG results were analyzed by single factor ANOVA. Results: 1) compared with the normal control group, there were three groups of DM rats with different intervention: The amplitude of a wave B wave was decreased by 1ERG. 2HE staining of retinal sections showed that the outer nuclear layer of the retina became loose and thin; 3 the ultrastructure disorder of the cells showed apoptosis in ultrathin retina sections by transmission electron microscope (TEM). (2) compared with DM group and DM DMSO group, the findings of DM CsA group were as follows: The amplitude of 1ERG a wave and b wave decreased slightly (P < 0.05). 2the degree of loose and thinning of outer nuclear layer is light; 3 the degree of ultrastructure change was slight. Conclusion: The retinal nerve function was damaged in the early stage of diabetic rats, and the retinal tissue structure was disordered and the ultrastructure of nerve cells was changed. Intravitreal injection of CsA can protect the function and structure of diabetic retina.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R774.1

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本文編號(hào)：1934398