本文選題：三七姜揮發(fā)油 + CNE-2細(xì)胞　； 參考：《桂林醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:目的是研究三七姜揮發(fā)油對(duì)人鼻咽癌CNE-2細(xì)胞侵襲和遷移的抑制作用,并探討其可能機(jī)制。方法:體外培養(yǎng)人鼻咽癌CNE-2細(xì)胞,CCK8實(shí)驗(yàn)檢測(cè)三七姜揮發(fā)油對(duì)CNE-2細(xì)胞增值能力的抑制作用,計(jì)算生長(zhǎng)抑制率及半數(shù)抑制濃度(IC50);選取三七姜揮發(fā)油(0,20,40,60μg·ml-1)作用于CNE-2細(xì)胞0,24,48h,分別于三個(gè)不同時(shí)間段,采用劃痕實(shí)驗(yàn)檢測(cè)三七姜揮發(fā)油對(duì)CNE-2細(xì)胞遷移能力的抑制作用;Transwell實(shí)驗(yàn)檢測(cè)三七姜揮發(fā)油對(duì)CNE-2細(xì)胞侵襲能力的抑制作用。取作用48h后的CNE-2細(xì)胞,采用蛋白印跡法檢測(cè)MMP-2、MMP-9和VEGF等侵襲和遷移相關(guān)基因蛋白水平的表達(dá);RT-PCR技術(shù)檢測(cè)MMP-2、MMP-9和VEGF等相關(guān)侵襲和遷移基因的表達(dá)情況。結(jié)果:三七姜揮發(fā)油作用于CNE-2細(xì)胞24h,48h,72h后,細(xì)胞半數(shù)抑制濃度分別為70.296μg·ml-1,63.749μg·ml-1,59.532μg·ml-1,呈時(shí)間-劑量依賴性(P0.05);不同濃度的三七姜揮發(fā)油(0,20,40,60μg·ml-1)作用于細(xì)胞48h后,劃痕實(shí)驗(yàn)發(fā)現(xiàn)與空白對(duì)照組相比,細(xì)胞的遷移能力隨著藥物濃度的增加而降低;Transwell實(shí)驗(yàn)顯示,隨著藥物濃度逐漸的增加,細(xì)胞的侵襲能力逐漸下降;RT-PCR顯示,與陰性對(duì)照組相比,MMP-2、MMP-9和VEGF等基因表達(dá)下調(diào),且變化趨勢(shì)呈一定的濃度依賴性。蛋白免疫印跡法顯示,與陰性對(duì)照組相比,MMP-2、MMP-9和VEGF等蛋白表達(dá)下調(diào),且變化趨勢(shì)呈一定的濃度依賴性。結(jié)論:三七姜揮發(fā)油能抑制人鼻咽癌CNE-2細(xì)胞侵襲和遷移的能力而發(fā)揮抗腫瘤的作用,并且成濃度依賴性,其機(jī)制可能與通過(guò)下調(diào)MMP-2、MMP-9和VEGF等基因的表達(dá),進(jìn)而抑制鼻咽癌腫瘤細(xì)胞的轉(zhuǎn)移有關(guān)。
[Abstract]:Aim: to study the inhibitory effect of volatile oil of Panax notoginseng on invasion and migration of human nasopharyngeal carcinoma (NPC) CNE-2 cells and its possible mechanism. Methods: the inhibitory effect of essential oil of Panax notoginseng on the proliferation of human nasopharyngeal carcinoma (NPC) CNE-2 cells was detected by CCK8 assay. The growth inhibition rate and the half inhibitory concentration (IC50) were calculated, and the essential oil of Radix Notoginseng was selected. The inhibitory effect of essential oil of Panax notoginseng on the migration of CNE-2 cells was detected by scratch test. The inhibitory effect of volatile oil of Panax notoginseng on the invasion of CNE-2 cells was detected by Transwell test. The expression of MMP-2, MMP-9, VEGF and other invasion and migration-related genes were detected by Western blotting in CNE-2 cells after 48 h. RT-PCR was used to detect the expression of invasion and migration genes such as MMP-2, MMP-9, VEGF and so on. Results: after treated with the volatile oil of Panax notoginseng for 24 h and 48 h and 72 h after treatment, the cell half inhibitory concentrations were 70.296 渭 g ml-1nl and 63.749 渭 g ml-1n 59.532 渭 g ml-1, respectively, in a time-dose dependent manner (P0.05A), and different concentrations of volatile oil 020404060 渭 g ml-1) were applied to the cells for 48 h. The migration ability of cells decreased with the increase of drug concentration. With the increase of drug concentration, the invasiveness of cells decreased gradually. Compared with negative control group, the expression of MMP-2, MMP-9, VEGF and other genes were down-regulated by reverse transcription-polymerase chain reaction (RT-PCR). The trend of change was in a concentration dependent manner. Western blot showed that the expression of MMP-2, MMP-9 and VEGF were down-regulated in a concentration dependent manner. Conclusion: the essential oil of Panax notoginseng can inhibit the invasion and migration of human nasopharyngeal carcinoma CNE-2 cells and play an antitumor role in a concentration-dependent manner. The mechanism may be related to the down-regulation of the expression of MMP-2, MMP-9 and VEGF genes. And then inhibit the metastasis of nasopharyngeal carcinoma tumor cells.
【學(xué)位授予單位】：桂林醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R739.63

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王俊;曲鐵兵;儲(chǔ)利勝;李琳;任翠翠;孫斯琪;方燕;;川芎嗪上調(diào)MMP-2、MMP-9表達(dá)促進(jìn)骨髓間充質(zhì)干細(xì)胞遷移[J];中國(guó)中西醫(yī)結(jié)合雜志;2016年06期

2 胡小勤;曾學(xué)文;林國(guó)彪;吳燕春;周蓓;韋少宣;孫健;劉艷婷;吳培;;益氣養(yǎng)陰方藥不同配伍對(duì)肺癌腫瘤血管生成影響的研究[J];中醫(yī)藥信息;2016年02期

3 趙婧暉;謝鯤鵬;隋佳琪;霍洪楠;謝明杰;;秦皮素通過(guò)抑制EGFR及其下游AKT信號(hào)通路抑制MCF-7細(xì)胞增殖及遷移[J];中國(guó)生物化學(xué)與分子生物學(xué)報(bào);2016年01期

4 郭穎;郭建華;符航;田芝瑜;羅強(qiáng);劉芳;張林西;;青蒿琥酯對(duì)人大腸癌Lovo細(xì)胞侵襲影響的初步研究[J];中國(guó)藥理學(xué)通報(bào);2016年01期

5 李志杰;韓世愈;于洋;劉磊;;莪術(shù)油對(duì)Hela細(xì)胞遷移及侵襲的初步實(shí)驗(yàn)研究[J];現(xiàn)代生物醫(yī)學(xué)進(jìn)展;2015年31期

6 單云龍;韋忠紅;陶麗;劉玉萍;盛曉波;陳晨;吳紅雁;陳文星;王愛云;陸茵;;川芎嗪抑制缺氧誘導(dǎo)的乳腺癌MDA-MB-435S細(xì)胞的侵襲及遷移[J];腫瘤;2015年06期

7 陳美娟;趙若琳;郭園園;寧利敏;張旭;;麥冬皂苷B對(duì)A549細(xì)胞株體外黏附、侵襲及遷移的抑制作用及機(jī)制研究[J];中國(guó)藥理學(xué)通報(bào);2015年05期

8 郝臻鳳;劉靜;張瑜;李瑤瑤;孔桂美;卜平;錢靜;;紫草素對(duì)宮頸癌Hela細(xì)胞增殖、侵襲和遷移的影響[J];中國(guó)實(shí)驗(yàn)方劑學(xué)雜志;2015年06期

9 徐佳;伍春蓮;黃杰;;雷公藤紅素通過(guò)影響Akt信號(hào)通路和整合素表達(dá)抑制肺癌細(xì)胞的轉(zhuǎn)移[J];中國(guó)中藥雜志;2015年06期

10 Jun Shi;Pin-Kang Wei;;Xiaotan Sanjie decoction inhibits interleukin-8-induced metastatic potency in gastric cancer[J];World Journal of Gastroenterology;2015年05期

相關(guān)博士學(xué)位論文 前4條

1 馮兵;羅勒多糖調(diào)節(jié)上皮間質(zhì)轉(zhuǎn)化抑制肝癌侵襲轉(zhuǎn)移的機(jī)制研究[D];廣州中醫(yī)藥大學(xué);2015年

2 陳翔;黃藥子抑制胃癌生長(zhǎng)轉(zhuǎn)移的實(shí)驗(yàn)研究[D];山東大學(xué);2014年

3 徐加英;血根堿抗宮頸癌作用及其機(jī)制的研究[D];蘇州大學(xué);2013年

4 陳永安;蟾毒靈抑制人肝癌細(xì)胞MHCC97H轉(zhuǎn)移的實(shí)驗(yàn)研究[D];第二軍醫(yī)大學(xué);2012年

相關(guān)碩士學(xué)位論文 前6條

1 呂文姣;益氣除痰方干預(yù)TGF-β信號(hào)途徑逆轉(zhuǎn)NSCLC上皮—間質(zhì)轉(zhuǎn)化的機(jī)制研究[D];廣州中醫(yī)藥大學(xué);2015年

2 陳道金;壯藥“國(guó)蝦薄”皂苷類成分對(duì)非小細(xì)胞肺癌侵襲轉(zhuǎn)移的影響[D];中央民族大學(xué);2015年

3 付健健;小檗堿干預(yù)CXCL12/CXCR4軸抑制結(jié)直腸癌侵襲轉(zhuǎn)移的作用及其可能機(jī)制的初步探索[D];昆明醫(yī)科大學(xué);2015年

4 季雪;南蛇藤提取物抑制人肝癌細(xì)胞HepG2轉(zhuǎn)移的相關(guān)機(jī)制研究[D];揚(yáng)州大學(xué);2014年

5 李東搏;130例鼻咽癌綜合治療療效及預(yù)后影響因素分析[D];大連醫(yī)科大學(xué);2014年

6 米日喀米力·玉蘇甫;VEGFR-2在鼻咽癌中的表達(dá)與PI3K/AKT信號(hào)通路相關(guān)性的初步研究[D];中南大學(xué);2013年

，

本文編號(hào)：1926649