本文選題：麥考酚酸 + 翼狀胬肉組織成纖維細(xì)胞�。� 參考：《濱州醫(yī)學(xué)院學(xué)報》2012年01期

【摘要】：目的研究麥考酚酸(MPA)對翼狀胬肉組織成纖維細(xì)胞(PFB)活性的影響。方法體外培養(yǎng)PFB及Tenon囊成纖維細(xì)胞(TFB),以絲裂霉素C(MMC)為對照,MTT比色法、5-溴脫氧尿嘧啶(5-bromo-2-deoxyuridine,Brdu)摻入法觀察MPA對PFB的生長抑制作用,以流式細(xì)胞術(shù)等檢測MPA對PFB、TFB的細(xì)胞毒性作用、細(xì)胞周期及凋亡的影響。結(jié)果 MPA可顯著抑制PFB、TFB的增殖,呈時間、劑量依賴性,且不引起TFB的凋亡或壞死。MPA作用后,PFB的G0/G1期比例增加,S期及G2/M期比例減少。結(jié)論 MPA對PFB有顯著的抑制作用,但不引起TFB的凋亡或壞死,且主要抑制在PFB的DNA合成期。
[Abstract]:Objective to study the effect of mycophenolic acid (MPA) on PFB activity in pterygium tissue fibroblasts. Methods PFB and Tenon capsule fibroblasts were cultured in vitro, and mitomycin C MMC was used as control. The inhibitory effect of MPA on the growth of PFB was observed by 5-bromo-2-deoxyuridine incorporation, and the cytotoxicity of MPA to PFB was detected by flow cytometry. Cell cycle and apoptosis. Results MPA could significantly inhibit the proliferation of TFB in a time-and dose-dependent manner, and did not induce apoptosis or necrosis of TFB. After treatment, the proportion of G0/G1 phase of PFB increased and the proportion of G _ 2 / M phase decreased. Conclusion MPA has a significant inhibitory effect on PFB, but does not induce apoptosis or necrosis of TFB, and is mainly inhibited in the DNA synthesis phase of PFB.
【作者單位】： 濱州醫(yī)學(xué)院眼科學(xué)教研室;濰坊眼科醫(yī)院與濰坊市眼科研究所;濱州醫(yī)學(xué)院流行病學(xué)教研室;濰坊醫(yī)學(xué)院眼科學(xué)教研室;
【分類號】：R777.33

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