發(fā)布時(shí)間：2018-05-21 09:41

  本文選題：慢性間隙性缺氧 + 氧化應(yīng)激��； 參考：《重慶醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的： 制作一個(gè)可應(yīng)用于研究正常壓力下慢性間隙性缺氧（Chronicintermittent hypoxia, CIH）的SD大鼠動(dòng)物模型，為研究睡眠呼吸疾病特征性的間隙性缺氧—復(fù)氧中的病理生理過程建立一個(gè)穩(wěn)定可靠的實(shí)驗(yàn)平臺(tái)。通過研究正常生活條件、慢性間隙性缺氧以及缺氧后復(fù)氧的情況下對(duì)SD大鼠的氧化應(yīng)激系統(tǒng)以及主動(dòng)脈血管內(nèi)皮系統(tǒng)的影響，探索睡眠呼吸疾病患者中氧化應(yīng)激系統(tǒng)紊亂與主動(dòng)脈內(nèi)皮細(xì)胞損傷的可能機(jī)制，為臨床疾病的治療提供思路。 方法： SD性成熟大鼠24只按照隨機(jī)數(shù)字法分為三組即：空白對(duì)照組（Unhandled control group）（正常生活環(huán)境飼養(yǎng)四周，UC組）、慢性間隙性缺氧組（Chronic intermittent hypoxia group）（慢性間隙性缺氧艙間缺氧四周，CIH組）以及復(fù)氧組（Removalof hypoxia group）（間隙性缺氧四周后再正常飼養(yǎng)四周，共八周，RH組）；所有大鼠造模成功后測(cè)定各組血清中氧化應(yīng)激指標(biāo)：丙二醛（Malondialdehyde, MDA）、超氧歧化物（Superoxide dismutase, SOD）、一氧化氮（Nitric oxide, NO）水平。放血法處死大鼠，取出主動(dòng)脈進(jìn)行HE染色，并用免疫組化方法觀察內(nèi)皮型一氧化氮合酶（Endothelialnitric oxide synthase, eNOS）的表達(dá)情況。 結(jié)果： 1、與UC組比較，CIH組血清的氧化指標(biāo)MDA含量顯著升高，SOD、NO含量明顯降低，差別有統(tǒng)計(jì)學(xué)意義（P0.05）。RH組與UC組比較，MDA、NO、SOD含量均無(wú)明顯差異，差別無(wú)統(tǒng)計(jì)學(xué)意義（p0.05）。 2、主動(dòng)脈HE染色：光鏡下見UC組血管內(nèi)皮完整、連續(xù)、光滑，內(nèi)皮細(xì)胞無(wú)脫落，內(nèi)膜、中膜、外膜分界清楚。CIH組與UC組比，內(nèi)皮細(xì)胞部分脫落，主動(dòng)脈內(nèi)膜不完整，，部分中膜平滑肌增生，排列紊亂，少部分平滑肌向內(nèi)膜層突起。RH組鏡下形態(tài)基本同UC組，內(nèi)膜細(xì)胞完整，平滑肌彈力纖維層排列整齊，中膜層未見明顯突起。 3、主動(dòng)脈免疫組化發(fā)現(xiàn)： UC組及RH組均可見棕黃色顆粒位于主動(dòng)脈內(nèi)皮細(xì)胞胞漿中，CIH組內(nèi)皮染色較淺，棕黃色顆粒較少。免疫組化半定量分析結(jié)果：CIH組的平均灰度值明顯低于UC組（p0.05）,RH組與UC組平均灰度值比較無(wú)明顯差異（p0.05）。 結(jié)論： 1、慢性間隙性缺氧中SD大鼠氧化應(yīng)激系統(tǒng)被激活，缺氧后復(fù)氧能使其基本恢復(fù)至正常水平。 2、慢性間隙性缺氧過程損傷大鼠主動(dòng)脈內(nèi)皮細(xì)胞，并導(dǎo)致內(nèi)皮型一氧化氮表達(dá)減少。復(fù)氧可以使內(nèi)皮功能恢復(fù)至正常水平。
[Abstract]:Objective: A SD rat model of chronic interstitial hypoxia (CIH) under normal pressure was made to establish a stable and reliable experimental platform for the study of the pathophysiological process in the characteristic intermittent hypoxia reoxygenation of sleep respiratory diseases. By studying the effects of normal living conditions, chronic intermittent hypoxia and reoxygenation after hypoxia on oxidative stress system and aortic vascular endothelial system in SD rats, To explore the possible mechanism of oxidative stress system disorder and aortic endothelial cell injury in patients with sleep apnea disease, and to provide ideas for the treatment of clinical diseases. Methods: Twenty-four SD sexual mature rats were divided into three groups according to the random number method: the blank control group was unhandled control group (normal living environment was fed for four weeks) and the chronic interstitial hypoxia group was chronic intermittent hypoxia group (chronic intermittent hypoxia for four weeks). In the reoxygenation group, the hypoxia group was fed normally for four weeks after intermittent hypoxia. The levels of serum oxidative stress (MDA), malondialdehyde (MDA), malondialdehyde (MDAA), superoxide dismutase (SOD), nitric oxide (no) were measured in all rats. The rats were killed by bloodletting, the aorta was removed for HE staining, and the expression of Endothelia oxide synthase, eNOS) was observed by immunohistochemical method. Results: 1.Compared with UC group, the content of serum MDA increased significantly, and the content of MDA decreased significantly. There was no significant difference between RH group and UC group, but there was no significant difference between RH group and UC group. 2HE staining of aorta: under light microscope, the vascular endothelium in UC group was intact, continuous, smooth, endothelium cells were not exfoliated, intima, media and adventitia boundaries were clear. Compared with UC group, the endothelial cells in CIH group were partially exfoliated, and the aortic intima was incomplete. Some of the medial smooth muscle proliferated and arranged disorderly. A few of the smooth muscle cells were similar to the UC group under microscope. The intimal cells were intact, the smooth muscle elastic fiber layer was arranged neatly, and there was no obvious protuberance in the middle layer. 3. The results of immunohistochemistry of aorta showed that brown granules were found in endothelial cytoplasm of aortic endothelial cells in UC group and RH group. In CIH group, the staining of endothelium was lighter and the brown granules were less. The results of semi-quantitative immunohistochemical analysis showed that the average gray value of the group of CIH was significantly lower than that of the group of UC (P 0.05) and that of the group of UC was not significantly different from that of the group of UC (P 0.05). Conclusion: 1. The oxidative stress system of SD rats was activated during chronic interstitial hypoxia. 2, chronic interstitial hypoxia injures aorta endothelial cells and reduces the expression of endothelial nitric oxide (no). Reoxygenation can restore endothelial function to normal level.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R766.4

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 王菁,王廣發(fā),凌亦凌;成年Sprague-Dawley大鼠的睡眠結(jié)構(gòu)和呼吸暫停分析[J];中國(guó)病理生理雜志;2005年03期

2 陳聆;李敏;;阻塞性睡眠呼吸暫停低通氣綜合征患者的氧化應(yīng)激狀態(tài)和血管內(nèi)皮的改變[J];診斷學(xué)理論與實(shí)踐;2009年03期

3 王璋;司良毅;廖友斌;;大鼠睡眠呼吸暫停綜合征動(dòng)物模型的建立[J];中國(guó)實(shí)驗(yàn)動(dòng)物學(xué)報(bào);2006年01期

4 陳寶元,何權(quán)瀛;提高我國(guó)睡眠呼吸疾病領(lǐng)域的整體水平[J];中華結(jié)核和呼吸雜志;2003年05期

相關(guān)會(huì)議論文 前1條

1 韓德民;葉京英;;睡眠呼吸疾病研究進(jìn)展與手術(shù)治療[A];第三屆全國(guó)睡眠學(xué)術(shù)會(huì)議論文匯編[C];2004年

本文編號(hào)：1918683