本文選題：葡萄籽原花青素 + 亞硒酸鈉性白內(nèi)障　； 參考：《華中科技大學(xué)》2012年博士論文

【摘要】：目的:探討不同劑量葡萄籽原花青素對大鼠硒性白內(nèi)障的抑制作用 方法:將80只8日齡的SD大鼠隨機分為對照組模型組和GSPE低中高劑量組對照組給予生理鹽水皮下注射，模型組于第10日齡起給予亞硒酸鈉20μmol/kg頸背部皮下注射，隔日1次，連續(xù)3次GSPE各組除給予同樣造模外，從第8日齡起還分別給予低劑量組50mg/kg中劑量組100mg/kg高劑量組200mg/kg的GSPE灌胃，每天1次連續(xù)14天待乳鼠睜眼后用裂隙燈顯微鏡觀察晶狀體的渾濁程度，分級以及拍照，并每天測量晶狀體核性渾濁斑塊的最大直徑變化光鏡下HE染色觀察各組晶狀體組織的細(xì)胞形態(tài) 結(jié)果:模型組全部形成典型的核性白內(nèi)障，造模成功率100%與模型組相比，GSPE各組的晶狀體渾濁程度和核性渾濁斑塊的最大直徑明顯減小（P0.05或P0.01或P0.001）光鏡下HE染色發(fā)現(xiàn)GSPE干預(yù)后，晶狀體組織的損傷程度減輕，且與GSPE有劑量關(guān)系 結(jié)論:該實驗證實了GSPE能明顯抑制亞硒酸鈉引起的核性白內(nèi)障的形成和發(fā)展，且有時間 劑量關(guān)系，其最低有效劑量為50mg/kg 目的:探討葡萄籽原花青素提取物抑制大鼠硒性白內(nèi)障的機理研究 方法:將80只8日齡的SD大鼠隨機分為對照組模型組GSPE低中高劑量組對照組給予生理鹽水皮下注射，模型組于第10日齡起給予亞硒酸鈉20μmol/kg頸背部皮下注射，隔日1次，連續(xù)3次GSPE各組除給予同樣造模外，從第8日齡起還分別給予低劑量組50mg/kg中劑量組100mg/kg高劑量組200mg/kg的GSPE灌胃，每天1次連續(xù)14天實驗結(jié)束后測量各組晶狀體中丙二醛(MDA)超氧化物歧化酶（SOD）過氧化氫酶（CAT）谷胱甘肽過氧化物酶（GSH-PX）一氧化氮（NO）鈣離子（Ca~(2+)）含量及晶狀體組織抑制羥自由基(OH-)的能力用免疫組織化學(xué)法觀察iNOS calpainⅡ蛋白在各組晶狀體上皮細(xì)胞中（LECs）的表達情況，并用平均吸光度(AOD)反應(yīng)各組的表達量用實時熒光定量RT-PCR法檢測iNOS calpainⅡmRNA在各組晶狀體中的表達情況 結(jié)果:與對照組比，模型組晶狀體中抗氧化酶（SOD CAT GSH-PX）的活性及抑制羥自由基的能力顯著下降，同時MDA Ca~(2+) NO的含量和iNOS calpainⅡ蛋白吸光度及mRNA的表達水平顯著上升（P0.01或P0.001）；與模型組相比，，GSPE各組抗氧化酶（SOD CAT GSH-PX）的活性及抑制羥自由基的能力明顯升高，同時MDA Ca~(2+) NO的含量和iNOS calpainⅡ蛋白及mRNA的表達水平明顯下降（P0.05或P0.01或P0.001），且與GSPE有劑量依賴性 結(jié)論:該實驗證實了GSPE能顯著抑制亞硒酸鈉引起的核性白內(nèi)障的形成，其作用機制可能與增強抗氧化酶（SOD CAT GSH-PX）的活性，阻止脂質(zhì)過氧化產(chǎn)物MDA及自由基羥基的產(chǎn)生，以及抑制晶狀體中iNOS和calpainⅡ的激活有關(guān)
[Abstract]:Objective: to investigate the inhibitory effect of different doses of grape seed procyanidins on selenium induced cataract in rats. Methods: eighty 8-day-old SD rats were randomly divided into two groups: the control group and the low, medium and high dose group of GSPE. The model group was subcutaneously injected with sodium selenite 20 渭 mol/kg, once every other day, and the model group was given subcutaneous injection of sodium selenite 20 渭 mol/kg every other day. In addition to the same model, each GSPE group was given intragastric administration of low dose 50mg/kg medium dose 100mg/kg high dose 200mg/kg GSPE from the 8th day of age. Once a day for 14 consecutive days, the degree of opacity, grading and taking pictures of the lens were observed by slit lamp microscope after the newborn rats opened their eyes. The maximum diameter change of lens nuclear opacification plaque was measured every day. The morphology of lens tissue in each group was observed by HE staining under light microscope. Results: typical nuclear cataract was formed in all of the model group. Compared with the model group, the degree of lens opacification and the maximum diameter of the nuclear opacifying plaque in the model group were significantly reduced by 100% success rate (P 0.05 or P 0.01 or P 0.001). The results of HE staining under light microscope showed that the degree of lens opacification and the maximum diameter of nuclear opacity plaque in the model group were significantly decreased compared with the model group. The degree of lens injury was reduced and there was a dose-dependent relationship with GSPE. Conclusion: GSPE can inhibit the formation and development of nuclear cataract induced by sodium selenite in a dose-dependent manner, and the lowest effective dose is 50mg/kg. Objective: to investigate the mechanism of grape seed proanthocyanidin extract (GPC) inhibiting rat selenium-induced cataract. Methods: eighty 8-day-old SD rats were randomly divided into two groups: the control group, the low, medium and high dose group of GSPE, the control group were subcutaneously injected with normal saline, and the model group was given subcutaneous injection of sodium selenite 20 渭 mol/kg into the neck and back from the 10th day, once every other day. In addition to the same model, each GSPE group was given intragastric administration of low dose 50mg/kg medium dose 100mg/kg high dose 200mg/kg GSPE from the 8th day of age. The content of malondialdehyde (MDAs) superoxide dismutase (SOD) and catalase (CAT) glutathione peroxidase (GSH-PX) (no) calcium in the lens of each group were measured once a day for 14 days. The expression of iNOS calpain 鈪

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