本文選題：年齡相關(guān)性白內(nèi)障 + 20s蛋白酶體α2亞基　； 參考：《皖南醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:通過對不同混濁程度及不同核硬度的年齡相關(guān)性白內(nèi)障(Age-related cataract,ARC)患者的晶狀體前囊膜20s蛋白酶體α2亞基的蛋白表達(dá)進(jìn)行檢測分析,以期了解不同混濁程度及不同核硬度的白內(nèi)障患者其晶狀體老化與20s蛋白酶體α2亞基的蛋白表達(dá)變化之間的關(guān)系及意義。方法:收集行超聲乳化或小切口白內(nèi)障摘除術(shù)的前囊膜120例(核性60例,皮質(zhì)性60例)。根據(jù)晶狀體核的硬度及混濁程度的不同進(jìn)行分組,分為核性組(60例60眼)和皮質(zhì)組(60例60眼)其中核性組按Emery分級標(biāo)準(zhǔn)分為兩組,即A組:年輕核(I-II度),B組:老核(IV-V度);皮質(zhì)組按LOCSII標(biāo)準(zhǔn)分為兩組,即A組:輕度混濁(C0-C2),B組:重度混濁(C4-C5)。應(yīng)用Western-Blotting法檢測20s蛋白酶體α2亞基在核性組和皮質(zhì)組ARC患者晶狀體前囊膜中的蛋白表達(dá)水平。結(jié)果:核性組白內(nèi)障中,A組中20s蛋白酶體α2亞基蛋白表達(dá)較B組增強(qiáng)((0.99±0,02)VS(0.58±0.08)),差異有統(tǒng)計(jì)學(xué)意義(P0.01);皮質(zhì)組白內(nèi)障中,A組中20s蛋白酶體α2亞基蛋白表達(dá)較B組增強(qiáng)((1.26±0,15)VS(0.89±0.09)),差異有統(tǒng)計(jì)學(xué)意義(P0.05);也即表示核性白內(nèi)障中年輕核比老核20s蛋白酶體α2亞基的蛋白表達(dá)含量高,皮質(zhì)白內(nèi)障中輕度混濁白內(nèi)障比重度混濁白內(nèi)障20s蛋白酶體α2亞基的蛋白表達(dá)含量高。結(jié)論:ARC患者前囊膜中20s蛋白酶體α2亞基的蛋白表達(dá)含量隨晶狀體混濁程度及核的硬度加深而降低。
[Abstract]:Objective: to study the expression of proteasome 偽 2 subunit in anterior capsule of lens in patients with age-related cataract (Age-related cataract) with different degree of opacity and nuclear hardness. The purpose of this study was to investigate the relationship between lens aging and proteasome 偽 2 subunit protein expression in cataract patients with different opacity and nuclear hardness. Methods: 120 cases of anterior capsule (60 nuclear and 60 cortical) underwent phacoemulsification or small incision cataract extraction. According to the hardness of lens nucleus and the degree of opacity, they were divided into two groups: nuclear group (60 cases, 60 eyes) and cortical group (60 cases, 60 eyes). The nuclear group was divided into two groups according to Emery grading standard. Group A was divided into two groups according to LOCSII standard: group A: young nucleus: group B: IV-V degree of old nucleus, group A: mild turbidity: group C 0-C 2: group B: severe opacification: C4-C5. The expression of 偽 2 subunit of 20 s proteasome in anterior capsule of lens of ARC patients in nuclear and cortical groups was detected by Western-Blotting method. Results: the expression of proteasome 偽 2 subunit protein in group A was significantly higher than that in group B (0.99 鹵0 02VSN 0.58 鹵0.08), and that in group A was higher than that in group B (1.26 鹵0 015VSU 0.89 鹵0.09), and the expression of proteasome 偽 2 subunit in group A was significantly higher than that in group B (P < 0. 01), and the expression of proteasome 偽 2 subunit in group A was significantly higher than that in group B (P < 0. 01). The protein expression of 偽 2 subunit in the young nucleus is higher than that in the old nucleus, which means that the protein expression of 偽 2 subunit in the young nucleus is higher than that in the old nucleus. The protein expression of proteasome 偽 2 subunit in mild cataract was higher than that in severe cataract. Conclusion the protein expression of proteasome 偽 2 subunit in the anterior capsule of the patients with 1% ARC decreased with the degree of lens opacity and the hardness of nucleus.
【學(xué)位授予單位】：皖南醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R779.66

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