本文選題：靜息膜電位 + 視皮層　； 參考：《天津醫(yī)科大學》2012年碩士論文

【摘要】：目的 研究正常光線條件下飼養(yǎng)及暗飼養(yǎng)條件下,不同年齡段大鼠視皮層Ⅱ/Ⅲ層錐體神經元靜息膜電位變化趨勢,比較兩組大鼠視皮層Ⅱ/Ⅲ層錐體神經元靜息膜電位的差異。 方法 將正常光線條件下飼養(yǎng)wistar大鼠分為五個年齡段,即p10-12d(睜眼前組),p14-16d(前關鍵期組),p19-21d(關鍵期組),p35-40d(后關鍵期組)及大于p60d(成年組),應用紅外微分干涉相差顯微鏡(IR-DIC)結合電耦合式攝像機(CCD-Camera)可視法膜片鉗全細胞模式下進行實驗,記錄健康wistar大鼠離體腦片視皮層Ⅱ/Ⅲ層錐體神經元靜息膜電位。 將暗飼養(yǎng)的wistar大鼠分為三個年齡段,即p10-12d(睜眼前組),p14-16d(前關鍵期組),p19-21d(關鍵期組),應用紅外微分干涉相差顯微鏡(IR-DIC)結合電耦合式攝像機(CCD-Camera)可視法膜片鉗全細胞模式下進行實驗,記錄暗飼養(yǎng)wistar大鼠離體腦片視皮層Ⅱ/Ⅲ層錐體神經元靜息膜電位,并與相對應年齡段健康wistar大鼠靜息膜電位進行比較。 結果 1.正常光線條件下飼養(yǎng)的各組大鼠視皮層Ⅲ層錐體神經元靜息膜電位為：pl0-12d(睜眼前組)(-45.665±6.848)mv,p14-16d(前關鍵期組)(-67.174±6.909)mv,p19-21d(關鍵期組)(-72.922±5.302)mv,p35-40d(后關鍵期組)(-77.294±4.361)mv,大于p60d(成年組)(-78.375±3.695)mv。隨年齡增長,大鼠視皮層Ⅱ/Ⅲ層錐體神經元靜息電位逐漸降低,在視覺發(fā)育關鍵期后,達到平臺期,接近成年水平(關鍵期后組和成年組靜息電位值無統(tǒng)計學意義,ANOVA,P0.05;其余組之間均有統(tǒng)計學差異,ANOVA,P0.05). 2.各組暗飼養(yǎng)大鼠視皮層Ⅱ/Ⅲ層錐體神經元靜息膜電位為：p10-12d(睜眼前組)(-53.147±4.364)mv,p14-16d(前關鍵期組)(-60.008±8.082)mv,p19-21d(關鍵期組)(-58.019±7.698)mv,p14-16d(前關鍵期組)視皮層Ⅱ/Ⅲ錐體神經元靜息膜電位較p10-12d(睜眼前組)更負,有統(tǒng)計學差異(ANOVA,P=0.012,P0.05)。而p14-16d(前關鍵期組)及p19-21d(關鍵期組)的視皮層Ⅱ/Ⅲ層錐體神經元細胞的靜息膜電位相比較,無統(tǒng)計學差異(ANOVA,P=0.454,P0.05)。 3.正常光線條件下飼養(yǎng)及暗飼養(yǎng)組大鼠在p10-12d(睜眼前組),p14-16d(前關鍵期組),p19-21d(關鍵期組)三個年齡段,視皮層Ⅱ/Ⅲ錐體神經元靜息膜電位隨著天齡的增加而逐漸變負,暗飼養(yǎng)大鼠天齡增加,視皮層Ⅱ/Ⅲ錐體神經元靜息膜電位變化幅度減小,明顯小于正常光線條件下飼養(yǎng)組大鼠。 結論 1.正常光線條件下飼養(yǎng)組大鼠的靜息電位變化趨勢：當視覺發(fā)育關鍵期未結束時,視皮層Ⅱ-Ⅲ層錐體神經元靜息膜電位隨著天齡的增加逐漸變負,而當關鍵期過后,視皮層Ⅱ-Ⅲ層錐體神經元靜息膜電位則穩(wěn)定下來,達到了成熟水平。暗飼養(yǎng)組大鼠視皮層Ⅱ-Ⅲ層錐體神經元靜息膜電位也有逐漸變負的趨勢,變化幅度減小,處于未成熟水平。 2.將正常光線條件下飼養(yǎng)組大鼠與暗飼養(yǎng)大鼠的p10-12d(睜眼前組),p14-16d(前關鍵期組)及p19-21d(關鍵期組)年齡段的靜息膜電位進行比較,正常光線條件下飼養(yǎng)組大鼠的靜息膜電位變化幅度遠大于暗飼養(yǎng)組大鼠,表明在視覺發(fā)育過程中,視覺經驗是促進視覺發(fā)育成熟的關鍵因素。在視覺系統(tǒng)發(fā)育的早期階段,為了促進自身的成熟,視覺系統(tǒng)對外界的刺激敏感,使錐體神經元之間形成有效的聯(lián)接,有效的突觸修飾也開始進行。當視覺發(fā)育關鍵期過后,視覺系統(tǒng)發(fā)育成熟,對外界刺激的敏感度降低。視皮層Ⅱ/Ⅲ層錐體神經元靜息膜電位趨于一致,形成平臺期。暗飼養(yǎng)干擾了上述過程,視皮層Ⅱ/Ⅲ層錐體神經元靜息膜電位維持在未成熟狀態(tài)。
[Abstract]:objective
The change trend of resting membrane potential in the visual cortex II / III pyramidal neurons of different age rats was studied under normal light conditions under the condition of feeding and dark feeding, and the difference of resting membrane potential in the two groups of rat visual cortex II / III pyramidal neurons was compared.
Method
Wistar rats were divided into five age groups under normal light conditions, namely, p10-12d (open and front group), p14-16d (pre critical stage group), p19-21d (key stage group), p35-40d (post critical period group) and greater than p60d (adult group), using infrared differential interference phase contrast microscope (IR-DIC) combined with electrically coupled camera (CCD-Camera) visual patch clamp whole cell The resting membrane potential of the second / third pyramidal neurons in the visual cortex of healthy Wistar rats was recorded.
The dark reared Wistar rats were divided into three age groups, namely, p10-12d (front and front groups), p14-16d (pre critical stage group) and p19-21d (critical phase group). The infrared differential interference phase contrast microscope (IR-DIC) was used in conjunction with the electrically coupled camera (CCD-Camera) visual patch clamp whole cell model to record the dark feeding of the isolated brain slices of Wistar rats. Resting membrane potential of cortical II / III pyramidal neurons was compared with resting membrane potential of healthy Wistar rats at corresponding age.
Result
1. the resting membrane potential of the third layer pyramidal neurons in the visual cortex of the rats was pl0-12d (-45.665 + 6.848) MV, p14-16d (-67.174 6.909) MV, p19-21d (critical period group) (-72.922 + 5.302) MV, p35-40d (-77.294 4.361) MV, greater than that of adult (adult group) (adult group). With the increase of age, the resting potential of the pyramidal neurons in the visual cortex of the rat was gradually reduced. After the critical stage of visual development, the resting potential of V. reached the stage of platform, which was close to the adult level (the resting potential value of the post critical group and the adult group was not statistically significant, ANOVA, P0.05; the other groups were statistically different, ANOVA, P0.05).
2. the resting membrane potential of the visual cortex II / III pyramidal neurons in the dark rearing rats was p10-12d (-53.147 + 4.364) MV, p14-16d (-60.008 + 8.082) MV, p19-21d (critical phase group) (-58.019 + 7.698) MV, and p14-16d (before the key stage group) the resting membrane potential of the neurons in the visual cortex II / III pyramidal neurons was more than that of the p10-12d (open eyes). Negative, there were statistical differences (ANOVA, P=0.012, P0.05). But there was no statistical difference between the resting membrane potential of the pyramidal neurons in the visual cortex of the visual cortex (the key stage group) and the p19-21d (the key stage group). There was no statistical difference (ANOVA, P=0.454, P0.05).
3. in the normal light condition, the rats in the feeding group and the dark rearing group were in p10-12d (open eyes), p14-16d (the pre critical stage group) and the three age groups of the p19-21d (key stage group). The resting membrane potential of the neurons in the visual cortex II / III pyramidal neurons gradually became negative with the increase of the age. The age of the dark rearing rats increased and the resting membrane potential of the neurons in the visual cortex II / III pyramidal neurons changed. The extent of reduction was significantly smaller than that of rats fed normal light.
conclusion
The resting potential change trend of rats in 1. normal light conditions: when the critical stage of visual development did not end, the resting membrane potential of the pyramidal neurons of the layer II - III layer of the visual cortex gradually became negative with the increase of age, while the static membrane potential of the pyramidal neurons of the layer II - III of the visual cortex stabilized and reached the maturity level after the critical period. The resting membrane potential of the second - and third pyramidal neurons in the visual cortex of the dark feeding group also showed a gradual negative trend, with a decreasing amplitude and at an immature level.
2. compared with the resting membrane potential of p10-12d (open eyes), p14-16d (pre critical period group) and p19-21d (key stage) age group, the resting membrane potential of the rats in the normal light condition was much greater than that in the dark rearing group. Visual experience is the key factor in promoting the maturity of visual development. In the early stage of visual system development, in order to promote its maturity, the visual system is sensitive to the external stimuli, make the pyramidal neurons form an effective connection, and the effective synaptic modification begins. The sensitivity of external stimulation decreased. The resting membrane potential of the pyramidal neurons in the visual cortex was consistent and formed a platform phase. Dark feeding interfered with the above process. The resting membrane potential of the pyramidal neurons in the layer II / III of the visual cortex was maintained in the immature state.

【學位授予單位】：天津醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R77

【共引文獻】

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本文編號：1849682