本文選題：鼻咽癌細胞系 + 海帶多糖�。� 參考：《廣西醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的鼻咽癌(nasopharyngeal carcinoma,NPC)是中國南方地區(qū)最常見的頭頸部惡性腫瘤,主要治療方法是以放射治療為主輔以化學(xué)藥物治療的綜合治療,而化學(xué)藥物治療對中晚期的鼻咽癌患者尤為重要,但治療劑量的化療藥物多有較多嚴重的毒副作用。海帶多糖(Laminaria Japonica Polysaccharides,LJP)是從海帶中分離提取出的具有生物活性的植物多糖,具有抗腫瘤、提高免疫功能、防輻射等多種活性功能,本研究旨在通過觀察海帶多糖在體內(nèi)外對人鼻咽癌細胞的抑制作用,探討其可能的抗癌機制。 方法運用MTT法檢測不同濃度的海帶多糖對人鼻咽癌細胞株(HONE1和CNE2)增殖的抑制作用。運用Annexin V-FITC/PI雙染法檢測海帶多糖對HONE1細胞凋亡的影響。以人鼻咽癌細胞株HONE1建立裸鼠皮下移植瘤模型,將造模成功的裸鼠隨機分入5個處理組：生理鹽水組、順鉑組、海帶多糖低、中、高劑量組,每組6只,觀察各組移植瘤體積和裸鼠體重的變化情況。2周治療結(jié)束后,處死裸鼠,剖出瘤塊,稱瘤重并計算各組的抑瘤率,并在透射電鏡(Transmission electron microscopy,TEM)觀察移植瘤細胞超微結(jié)構(gòu)的改變。 結(jié)果MTT結(jié)果顯示,海帶多糖對鼻咽癌細胞(HONE1和CNE2)的增殖有抑制作用,且具有劑量相關(guān)性,320mg/L的海帶多糖作用72h的抑制率分別為58.27%(P0.01)和55.00%(P0.01)；流式細胞術(shù)(Flow Cytometry, FCM)檢測結(jié)果表明,海帶多糖具有誘導(dǎo)HONE1細胞凋亡的作用,凋亡率隨著海帶多糖濃度的增加而增高,320mg/L的凋亡率為(49.51±1.89)%(P0.01)。體內(nèi)實驗中,海帶多糖中、高劑量組抑瘤率分別為33.7%(P0.05)和47.0%(P0.01),具有明顯抑制移植瘤的作用,而低劑量組的抑瘤率僅為16.4%(P0.05)。透射電鏡結(jié)果提示移植瘤組織的癌細胞呈現(xiàn)凋亡獨特的改變：細胞皺縮、染色質(zhì)凝聚、胞核碎裂、胞漿空泡變性、胞膜起泡、形成游離的凋亡小體。 結(jié)論海帶多糖具有抑制鼻咽癌細胞生長的作用,機制可能是通過誘導(dǎo)癌細胞凋亡而實現(xiàn)的。
[Abstract]:Objective nasopharyngeal carcinoma (NPC) is the most common malignant tumor of head and neck in southern China. The main treatment method is radiotherapy combined with chemotherapeutic therapy, and chemotherapy is especially important for patients with advanced nasopharyngeal carcinoma. However, the therapeutic dose of chemotherapeutic drugs have more serious side effects. Laminaria Japonica Polysaccharide (Laminaria Japonica Polysaccharide) is a bioactive plant polysaccharide isolated from Laminaria japonica. The aim of this study was to observe the inhibitory effect of kelp polysaccharides on human nasopharyngeal carcinoma cells in vitro and in vivo, and to explore its possible anticancer mechanism. Methods the inhibitory effects of different concentrations of kelp polysaccharides on the proliferation of human nasopharyngeal carcinoma cell lines (HONE1 and CNE2) were detected by MTT assay. The effect of kelp polysaccharide on apoptosis of HONE1 cells was detected by Annexin V-FITC/PI double staining. Human nasopharyngeal carcinoma (NPC) cell line HONE1 was used to establish subcutaneous transplanted tumor model in nude mice. The successful nude mice were randomly divided into 5 groups: normal saline group, cisplatin group, kelp polysaccharide low, middle and high dose group, each group with 6 rats. After 2 weeks of treatment, nude mice were killed, tumor weight was weighed, tumor inhibition rate was calculated, and ultrastructural changes of transplanted tumor cells were observed under transmission electron microscope (TEM). Results MTT results showed that kelp polysaccharides could inhibit the proliferation of nasopharyngeal carcinoma cells (HONE1 and CNE2), and the inhibitory rates of kelp polysaccharides with dose-dependent manner were 58.27mg / L and 55.00% P0.01respectively, and the flow Cytometry (FCM) showed that the inhibitory rates of kelp polysaccharides were 58.27mg / L and 55.00g / L, respectively, and the results of flow Cytometry (FCM) showed that Kelp polysaccharides could induce apoptosis of HONE1 cells. The apoptotic rate increased with the increase of kelp polysaccharide concentration, and the apoptotic rate of 320mg / L was 49.51 鹵1.89g / L. In vivo experiments, the inhibitory rates of high dose kelp polysaccharides were 33.7 and 47.0, respectively, which could inhibit the transplanted tumor obviously, but in the low dose group, the tumor inhibition rate was only 16.4% and P0.05%. The results of transmission electron microscope showed that the cancer cells of transplanted tumor showed unique changes of apoptosis, such as cell shrinkage, chromatin condensation, nuclear fragmentation, cytosolic vacuolar degeneration, membrane bubbling, and free apoptotic bodies. Conclusion Laminaria polysaccharide can inhibit the growth of nasopharyngeal carcinoma cells, and the mechanism may be by inducing apoptosis of cancer cells.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R739.63

【參考文獻】

相關(guān)期刊論文 前10條

1 王庭欣,秦淑貞,趙文,蔣東升,馬曉彤,邊慶榮;海帶多糖對環(huán)磷酰胺誘發(fā)小鼠骨髓細胞微核率的抑制作用[J];癌變.畸變.突變;1999年02期

2 胡兵,魏于全;表皮生長因子受體靶向腫瘤生物治療[J];癌癥;2004年04期

3 張松,孔維佳,王彥君,韓月臣,張丹;5-雜氮-2′-脫氧胞苷對人鼻咽癌裸鼠移植瘤的抑制作用[J];癌癥;2005年10期

4 王洪俠;;海帶多糖的藥理研究進展[J];赤峰學(xué)院學(xué)報(自然科學(xué)版);2011年02期

5 梁杰珍;孫文忠;徐志文;曾曼麗;陳美球;;海帶多糖對放療后頜下腺細胞凋亡的影響[J];重慶醫(yī)學(xué);2011年15期

6 王曉宇;鄒明明;王蓉;張翠麗;鄒向陽;;海藻多糖抗腫瘤機理研究進展[J];大連醫(yī)科大學(xué)學(xué)報;2007年03期

7 張全斌,徐祖洪;褐藻多糖硫酸酯化學(xué)研究的進展[J];中國海洋藥物;1996年04期

8 錢風云,傅德賢,歐陽藩;海帶多糖生物功能研究進展[J];中國海洋藥物;2003年01期

9 錢風云,傅德賢,歐陽藩;海帶多糖功用研究進展(一)[J];精細與專用化學(xué)品;2003年07期

10 李小婷;殷辰俞;孟徐蓮;段頡;孔秋月;馮晴;;褐藻糖膠抑制人肝癌細胞HepG2增殖的機制[J];南京醫(yī)科大學(xué)學(xué)報(自然科學(xué)版);2011年09期

，

本文編號：1844577