本文選題：淋巴結(jié)示蹤劑 + 前哨淋巴結(jié)��； 參考：《昆明醫(yī)科大學》2017年碩士論文

【摘要】：[目的]:通過將淋巴結(jié)示蹤劑納米炭、亞甲藍、中華墨汁注射入活體兔喉腔內(nèi)聲門及聲門上區(qū),動態(tài)觀察其在兔喉部、頸部淋巴結(jié)染色情況,依此比較上述三種淋巴結(jié)示蹤劑在喉腔不同部位給藥條件下頸部淋巴結(jié)染色示蹤效果,為日后相關(guān)淋巴結(jié)示蹤劑在喉癌及其淋巴結(jié)轉(zhuǎn)移的患者中的臨床應用提供理論和實驗依據(jù)。[方法]:取新西蘭大白兔30只,隨機分成6組,每組5只,將實驗兔麻醉,經(jīng)口在電子喉鏡引導下使用喉針在兔聲門上部、聲門部分別注射納米炭、亞甲藍、中華墨汁。退出喉鏡后15min內(nèi)完成對實驗兔行頸部淋巴結(jié)的解剖,術(shù)中盡量減少對喉部淋巴管網(wǎng)的損傷,并隨時間動態(tài)觀察實驗兔喉部前哨淋巴結(jié)的染色數(shù)量,開始著色時間及染色消褪時間等數(shù)據(jù)并相互比對分析。[結(jié)果]:實驗組中納米炭組染色淋巴結(jié)最多共計36枚。亞甲藍組次之共染色33枚淋巴結(jié),中華墨汁組最少共染色7枚淋巴結(jié)。對于聲門上部注射組來說:亞甲藍組淋巴結(jié)染色平均數(shù)為(5.400± 1.140)枚、納米炭組為(7.400±1.140)枚,P0.05,兩組有統(tǒng)計學意義;而著色的時間上亞甲藍組為(20.400±1.673)min,納米炭組為(31.200±2.864)min,兩組比較有統(tǒng)計學意義(P0.05)。對于聲門部注射組來說:亞甲藍組染色淋巴結(jié)為(1.200±0.447)枚,納米炭組為(1.800±0.837)枚,兩組無明顯統(tǒng)計學意義(P=0.305);著色的時間上亞甲藍組為(102.200±3.564)min,納米炭組為(86.600±2.702)min,兩組相比較有統(tǒng)計學意義(P0.05)。[結(jié)論]:納米炭組和亞甲藍組均能將實驗兔喉部引流的前哨淋巴結(jié)染色,納米炭組淋巴結(jié)染色數(shù)較多,著色時間上各有差異,總體而言兩者各具優(yōu)缺點。而中華墨汁在喉部無論是染色淋巴結(jié)數(shù)量還是著色時間上均較亞甲藍和納米炭兩者效果差,不適合作為兔喉部淋巴結(jié)示蹤劑使用。組織被3種淋巴結(jié)示蹤劑上色后均不會影響標本的病理檢驗。
[Abstract]:[objective]: by injecting lymph node tracer carbon, methylene blue and Chinese ink into the glottis and supraglottic region of rabbit larynx in vivo, the staining of lymph nodes in rabbit larynx and neck was observed dynamically. According to the above mentioned three kinds of lymph node tracers, the effect of cervical lymph node staining on different parts of laryngeal cavity was compared. To provide theoretical and experimental basis for clinical application of lymph node tracer in patients with laryngeal carcinoma and lymph node metastasis. [methods] Thirty New Zealand white rabbits were randomly divided into 6 groups (5 rabbits in each group). The experimental rabbits were anesthetized with laryngeal needle under the guidance of electronic laryngoscope, and the glottis were injected with carbon nanoparticles, methylene blue and Chinese ink respectively under the guidance of electronic laryngoscope. After laryngoscope, the cervical lymph nodes of experimental rabbits were dissected in 15min, the injury of laryngeal lymphatic network was minimized during the operation, and the number of sentinel lymph nodes in larynx of experimental rabbits was observed dynamically over time. Start coloring time and dyeing fading time and other data and compare analysis with each other. Results: in the experimental group, 36 lymph nodes were stained. 33 lymph nodes were stained in methylene blue group and 7 lymph nodes were stained in Chinese ink group. For the upper glottic injection group, the mean number of lymph nodes staining was 5.400 鹵1.140 in methylene blue group and 7.400 鹵1.140 in nano-carbon group, which was statistically significant in both groups, but the staining time in methylene blue group was 20.400 鹵1.673 minutes, and that in nano-carbon group was 31.200 鹵2.864 minutes. For glottic injection group, the stained lymph nodes were 1.200 鹵0.447 in methylene blue group and 1.800 鹵0.837 in carbon nano-group. There was no significant difference between the two groups in the staining time of methylene blue group (102.200 鹵3.564min) and nano-carbon group (86.600 鹵2.702min). [conclusion] both nano-carbon group and methylene blue group can stain the sentinel lymph nodes draining from the larynx of experimental rabbits. The numbers of lymph nodes in the nano-carbon group are more, and the coloring time is different. Generally speaking, the two groups have their own advantages and disadvantages. Chinese ink is less effective than methylene blue and carbon nanoparticles in the number and time of lymph nodes staining in larynx, so it is not suitable to be used as tracer in rabbit laryngeal lymph nodes. Tissue coloring with three kinds of lymph node tracers did not affect the pathological examination of the specimens.
【學位授予單位】：昆明醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R739.65

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