本文選題：喉鱗癌 + GAP酶激活蛋白SH功能區(qū)結(jié)合蛋白(GBP)　； 參考：《細(xì)胞與分子免疫學(xué)雜志》2017年07期

【摘要】：目的研究RNA結(jié)合的Ras-GAP酶激活蛋白SH3結(jié)合蛋白(G3BP)、腫瘤干細(xì)胞標(biāo)志物CD44v6蛋白與喉鱗狀細(xì)胞癌(喉鱗癌)發(fā)生發(fā)展的關(guān)系,探討G3BP、CD44v6蛋白與血管新生的相關(guān)性。方法收集56例喉鱗癌患者癌組織及對應(yīng)癌旁組織,采用Western blot法檢測喉鱗癌患者癌組織及對應(yīng)癌旁組織中G3BP、CD44v6蛋白水平,使用免疫組織化學(xué)染色法分別檢測G3BP、CD44v6、血管內(nèi)皮生長因子A(VEGF-A)的表達(dá),并分析G3BP、CD44v6表達(dá)與喉鱗癌患者臨床病理特征的關(guān)系及與VEGF-A表達(dá)的相關(guān)性;采用Ⅷ因子相關(guān)抗原(FⅧAg)免疫組織化學(xué)染色確定微血管密度(MVD),觀察G3BP、CD44v6表達(dá)與MVD之間的關(guān)系。結(jié)果喉鱗癌患者癌組織中G3BP蛋白、CD44v6蛋白水平均明顯高于對應(yīng)癌旁的正常組織。喉鱗癌患者癌組織中的G3BP、CD44v6、VEGF-A表達(dá)陽性率分別為58.9%、53.6%、46.4%,均明顯高于對應(yīng)的癌旁正常組織。喉鱗癌患者癌組織中G3BP、CD44v6蛋白表達(dá)陽性率與臨床分期、復(fù)發(fā)或轉(zhuǎn)移及淋巴結(jié)轉(zhuǎn)移相關(guān),但與年齡和腫瘤大小無關(guān)。經(jīng)Pearson相關(guān)性分析,喉鱗癌患者癌組織中G3BP、CD44v6蛋白的表達(dá)與VEGF-A表達(dá)呈正相關(guān)(r=0.741,r=0.756);喉鱗癌患者癌組織中G3BP與CD44v6蛋白陽性表達(dá)病例的MVD值均明顯高于癌旁正常組織中MVD值;癌組織中G3BP與CD44v6蛋白陰性表達(dá)病例MVD值與癌旁正常組織中MVD值比較無明顯差異。結(jié)論喉鱗癌組織G3BP、CD44v6蛋白高表達(dá),可能通過促進(jìn)VEGF-A的表達(dá)來誘發(fā)腫瘤微血管的新生,促進(jìn)喉鱗癌侵襲、轉(zhuǎn)移。
[Abstract]:Objective to study the relationship between RNA binding Ras-GAP activator protein SH3 binding protein G3BP1, tumor stem cell marker CD44v6 protein and laryngeal squamous cell carcinoma (LSCC), and to explore the relationship between G3BP- CD44v6 protein and angiogenesis in laryngeal squamous cell carcinoma (LSCC). Methods A total of 56 cases of laryngeal squamous cell carcinoma (LSCC) and its adjacent tissues were collected. The levels of G3BP- CD44v6 protein in laryngeal squamous cell carcinoma (LSCC) tissues and adjacent tissues were detected by Western blot assay. The expression of CD44v6 and VEGF-A were detected by immunohistochemical staining. The relationship between the expression of CD44v6 and VEGF-A in laryngeal squamous cell carcinoma (LSCC) was analyzed. The microvessel density (MVD) was determined by immunohistochemical staining of F 鈪，

本文編號：1804111