本文選題：CRS + 嗅覺障礙�。� 參考：《南昌大學》2012年碩士論文

【摘要】：目的：觀察AMPA受體（alpha-Amino-3-hydroxy-5-methyl-4-isoxazole-propionic Acid，α-氨基羥甲基惡唑丙酸）在慢性鼻及鼻竇炎(chronic rhinosinusitis,CRS)伴嗅覺障礙大鼠嗅球中的表達變化，探討CRS伴嗅覺障礙的發(fā)病機制。 方法：采用鼻腔阻塞加肺炎鏈球菌接種的方法建立SD大鼠CRS伴嗅覺障礙模型。HE染色觀察嗅粘膜形態(tài)變化，食物小球埋藏法(buffed food pellet test，BFPT)評估大鼠嗅覺功能， RT-PCR及免疫熒光兩種方法檢測嗅球中AMPA受體的表達。 結(jié)果：對照組、造模4周組、8周組及12周組嗅粘膜出現(xiàn)急性到慢性的變化過程，包括粘膜層增厚，，腺體增加，中性粒細胞、漿細胞、淋巴細胞增加。在慢性炎癥過程中，淋巴細胞是主要浸潤細胞。BFPT檢測各模型組與對照組比較有差異，12周組出現(xiàn)明顯的嗅覺障礙（P0.01）。12周模型組中嗅球GluR2mRNA的表達較正常組明顯下降（P0.05）。免疫熒光檢測嗅球GluR2表達在12周組熒光強度弱于前3組。與PCR結(jié)果一致。 結(jié)論：采用鼻腔阻塞加肺炎鏈球菌接種的方法可以成功建立CRS嗅覺障礙的大鼠模型。AMPA受體的表達下降可能參與CRS引起嗅覺障礙的發(fā)病機制。
[Abstract]:Objective: To observe the changes in the expression of AMPA receptor (alpha-Amino-3-hydroxy-5-methyl-4-isoxazole-propionic Acid, alpha aminomethyloxaloxalpropionic acid) in the olfactory bulb of rats with chronic rhinosinusitis (chronic rhinosinusitis, CRS) with olfactory disorder, and to explore the pathogenesis of the olfactory disorder in CRS.
Methods: using the method of nasal obstruction and Streptococcus pneumoniae inoculation, the morphologic changes of olfactory mucosa were observed by.HE staining of CRS with olfactory disorder model in SD rats. The olfactory function of rats was evaluated by buffed food pellet test (BFPT), and the expression of AMPA receptors in the olfactory bulb was detected by two methods of RT-PCR and immunofluorescence.
Results: in the control group, the olfactory mucosa of the 4 weeks group, the 8 week group and the 12 week group appeared acute to chronic changes, including the thickening of the mucous membrane, the increase of the gland, the increase of the neutrophils, plasma cells and lymphocytes. In the process of chronic inflammation, the lymphocyte was the main infiltrating cell.BFPT in each model group compared with the control group, and in the 12 week group. The expression of olfactory bulb GluR2mRNA in the.12 week model group of P0.01 was obviously lower than that in the normal group (P0.05). The fluorescence intensity of the olfactory bulb GluR2 expression in the 12 week group was weaker than the first 3 groups, and the results were consistent with the results of PCR.
Conclusion: using nasal obstruction and Streptococcus pneumoniae inoculation, the decrease of.AMPA receptor expression in rat model of CRS olfactory disorder may be involved in the pathogenesis of CRS induced olfactory disorder.

【學位授予單位】：南昌大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R765

【參考文獻】

相關(guān)期刊論文 前7條

1 張麗艷;盧滿存;林鵬;;慢性鼻-鼻竇炎患者嗅覺障礙的臨床分析[J];廣東醫(yī)學;2010年14期

2 趙正宏;郭軍;;AMPA受體與阿爾茨海默病[J];生命的化學;2010年02期

3 葉菁;余洪猛;李華偉;吉建;沈云珍;李雯;;運用Merocel止血棉與肺炎鏈球菌建立大鼠急性鼻腔鼻竇炎模型[J];中國眼耳鼻喉科雜志;2006年04期

4 姜濤;邊志剛;曹志偉;;嗅覺障礙患者鼻內(nèi)鏡術(shù)后嗅黏膜形態(tài)學觀察[J];中國耳鼻咽喉顱底外科雜志;2008年02期

5 何春燕,劉紅剛,王先忠,張盛忠;慢性鼻竇炎鼻息肉篩竇黏膜病理形態(tài)學研究[J];中華耳鼻咽喉科雜志;2003年02期

6 劉鋼;張海;;上呼吸道感染后嗅覺障礙患者嗅上皮超微結(jié)構(gòu)觀察[J];中華耳鼻咽喉頭頸外科雜志;2007年11期

7 董震;;慢性鼻及鼻竇炎的病理本質(zhì)及對策[J];中國醫(yī)學文摘(耳鼻咽喉科學);2006年03期

本文編號：1792718