本文選題：結(jié)膜杯狀細(xì)胞 + 細(xì)胞培養(yǎng)��； 參考：《中國老年學(xué)雜志》2017年07期

【摘要】：目的體外分離、培養(yǎng)大鼠結(jié)膜杯狀細(xì)胞,觀察其形態(tài)、結(jié)構(gòu)等生物學(xué)特征。方法取大鼠穹隆部結(jié)膜,加入含10%胎牛血清的RPMI 1640培養(yǎng)液中行組織塊培養(yǎng),5~7 d后酶消化法傳代。觀察原代及傳代細(xì)胞的形態(tài)及生長情況,并應(yīng)用特殊免疫組化染色方法分別對(duì)原代、傳代培養(yǎng)的杯狀細(xì)胞進(jìn)行鑒定,用Image J軟件計(jì)算細(xì)胞純度。結(jié)果倒置相差顯微鏡下可見杯狀細(xì)胞呈鵝卵石樣,5~7 d在組織塊周圍形成環(huán)狀結(jié)節(jié)。免疫組化結(jié)果顯示,培養(yǎng)的原代細(xì)胞角蛋白(CK)4染色陰性,CK7染色陽性,且CK7陽性細(xì)胞數(shù)占(97.3±2.0)%。傳代杯狀細(xì)胞中,絕大多數(shù)凝集素UEA-I染色為陽性,陽性細(xì)胞占(91.2±2.7)%。結(jié)論大鼠穹窿部結(jié)膜可以成功培養(yǎng)出杯狀細(xì)胞,傳代細(xì)胞仍保持原代細(xì)胞特性,且純度較高。
[Abstract]:Objective to isolate and culture rat conjunctival goblet cells in vitro and observe their morphological and structural characteristics. Methods the conjunctiva of rat fornix was cultured in RPMI 1640 medium containing 10% fetal bovine serum for 5 days and then subcultured by enzyme digestion. The morphology and growth of primary and passage cells were observed, and the primary and cultured goblet cells were identified by special immunohistochemical staining. The purity of the cells was calculated by Image J software. Results under inverted phase contrast microscope, goblet cells were found to form circular nodules around the tissue mass on the 7th day as cobblestone. The results of immunohistochemistry showed that the primary cultured cells were positive for CK7, and the number of CK7 positive cells accounted for 97.3 鹵2.0%. Most of the goblet cells were positive for lectin UEA-I, and the positive cells accounted for 91.2 鹵2.7. Conclusion the rat fornix conjunctiva can culture goblet cells successfully.
【作者單位】： 山西醫(yī)科大學(xué)第二醫(yī)院眼科;山西醫(yī)科大學(xué)第二醫(yī)院耳鼻喉科;
【基金】：山西省回國留學(xué)人員科研資助項(xiàng)目(2016-062)
【分類號(hào)】：R77

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王志昕;鄧世靖;羅時(shí)運(yùn);王智群;李然;王津津;孫旭光;;正常人結(jié)膜杯狀細(xì)胞的體外培養(yǎng)[J];眼科;2006年03期

2 柯碧蓮,佘振玨,肖虹蕾,胡寶洋,張皙,邱孝芝,周國民;人眼結(jié)膜杯狀細(xì)胞的定量研究[J];解剖學(xué)雜志;2001年03期

3 張悅;劉祖國;陳小平;張適;虞東芳;李朝陽;;維生素A對(duì)角膜移植排斥反應(yīng)后結(jié)膜杯狀細(xì)胞的影響[J];眼科學(xué)報(bào);2006年04期

4 杜園園;蘇冠方;劉早霞;王聰;;兔結(jié)膜杯狀細(xì)胞的無血清培養(yǎng)法[J];中國實(shí)驗(yàn)診斷學(xué);2007年08期

5 張悅;黃婉榮;張適;劉應(yīng)鵬;;維生素A對(duì)角膜移植排斥反應(yīng)后結(jié)膜杯狀細(xì)胞的影響(英文)[J];國際眼科雜志;2007年06期

6 邱曉，

本文編號(hào)：1790576