本文選題：阻塞性睡眠呼吸暫停低通氣綜合征 + 慢性間歇性低氧　； 參考：《中國呼吸與危重監(jiān)護雜志》2017年05期

【摘要】：目的研究CD73在間歇性低氧合并高脂飲食條件下大鼠心臟損傷中的表達及意義。方法建立大鼠慢性間歇性低氧合并高脂飲食模型。將SPF級健康雄性Wistar大鼠24只隨機分為4組,每組6只。A組(常氧及普通飲食);B組(常氧及高脂飲食);C組(間歇性低氧及普通飲食);D組(間歇性低氧及高脂飲食)。在實驗6周后,取各組大鼠血清檢測血脂(甘油三酯、低密度脂蛋白膽固醇)水平,光鏡下觀察大鼠心肌形態(tài)學變化,免疫組化和蛋白印跡檢測CD73蛋白在各組大鼠心肌細胞中的表達水平。結果 4組大鼠血脂水平比較,差異有統(tǒng)計學意義(均P0.05)。HE結果顯示:A組心肌細胞未見明顯異常;B組和C組中可見部分心肌纖維排列紊亂,局灶性變性壞死;D組心肌細胞損傷最為明顯,可見肌纖維排列紊亂,縱橫紋不清,部分肌纖維明顯溶解。免疫組化顯示,與A組比較,B、C、D組心肌細胞CD73蛋白表達水平均明顯升高(均P0.01);與B組和C組比較,D組CD73蛋白表達水平明顯升高(均P0.01)。蛋白印跡也顯示出一致的結果:與A組比較,B、C組CD73蛋白表達水平均顯著升高(均P0.05),D組CD73蛋白表達水平升高更為顯著(P0.01);與B組和C組比較,D組CD73蛋白表達水平明顯升高(均P0.01)。結論慢性間歇性低氧、高脂飲食可以引起心肌細胞的損傷和心肌細胞中CD73表達上調。
[Abstract]:Objective to study the expression and significance of CD73 in rat heart injury induced by intermittent hypoxia and high fat diet.Methods chronic intermittent hypoxia combined with high fat diet was established in rats.24 healthy male Wistar rats of SPF grade were randomly divided into 4 groups: group A (n = 6), group A (n = 6), group A (n = 6), group A (n = 6), group A (n = 6), group A (n = 6), group A (n = 6), group A (n = 6), group A (n = 6), group A (n = 6), group A (n = 6), group A (n = 6).After 6 weeks, serum lipids (triglycerides, low density lipoprotein cholesterol) were measured and myocardial morphologic changes were observed under light microscope.The expression of CD73 protein in rat cardiomyocytes was detected by immunohistochemistry and Western blot.Results there were significant differences in blood lipid levels among the four groups (P0.05).HE results showed that there were no significant abnormalities in cardiac myocytes in group B and group C, and some of the myocardial fibers were disordered in group B and C, respectively).In group D, myocardial cell damage was most obvious, muscle fibers were disordered, longitudinal and transverse lines were unclear, and some muscle fibers were obviously dissolved.Compared with group A, the expression of CD73 protein in cardiomyocytes was significantly increased (all P 0.01), and the expression of CD73 protein in group D was significantly higher than that in group B and C (all P 0.01).Western blotting also showed the same results: compared with group A, the expression of CD73 protein in group C was significantly higher than that in group A (P 0.05) and CD73 protein expression in group D was significantly higher than that in group B and C (P 0.01).Conclusion chronic intermittent hypoxia and high fat diet can induce myocardial injury and up-regulate the expression of CD73 in cardiomyocytes.
【作者單位】： 蘭州大學第一臨床醫(yī)學院;蘭州大學第一醫(yī)院呼吸科;
【基金】：甘肅省自然科學基金(1506RJZA254)
【分類號】：R766

【相似文獻】

相關期刊論文 前6條

1 羅勤;趙智慧;柳志紅;張洪亮;王勇;;慢性間歇性低氧對大鼠左心功能的影響及作用機制研究[J];中國分子心臟病學雜志;2012年01期

2 羅勤;柳志紅;趙智慧;張洪亮;;慢性間歇性低氧對大鼠左心室功能的影響[J];山東醫(yī)藥;2009年52期

3 夏洪芬;呂麟亞;張代英;田華菊;;慢性間歇性低氧對SD幼鼠生長發(fā)育影響的實驗研究[J];四川醫(yī)學;2010年06期

4 李霞;劉幼碩;詹俊鯤;羅熒荃;王翼;榮耀;楊宇;;慢性間歇性低氧和高脂飼養(yǎng)對兔胰島β細胞功能的影響[J];中國老年學雜志;2008年15期

5 趙翔翔;李雁鵬;趙忠新;;阻塞性睡眠呼吸暫停綜合征致中樞神經(jīng)系統(tǒng)損害[J];中國現(xiàn)代神經(jīng)疾病雜志;2013年05期

6 李渝;陳晨;丁少芳;王麗;;伴有阻塞性睡眠呼吸暫停低通氣綜合征的2型糖尿病患者的臨床特點分析[J];中國糖尿病雜志;2012年07期

相關會議論文 前1條

1 李秀翠;蔡曉紅;周永海;梁冬施;溫正旺;;內質網(wǎng)應激對慢性間歇性低氧幼鼠記憶損害的影響[A];2012年江浙滬兒科學術年會暨浙江省醫(yī)學會兒科學分會學術年會、兒內科疾病診治新進展國家級學習班論文匯編[C];2012年

相關博士學位論文 前1條

1 譚勝玉;慢性間歇性低氧對HDL代謝的影響及相關機制的研究[D];中南大學;2014年

相關碩士學位論文 前4條

1 李浩;HIF-1α、VEGF在阻塞性睡眠呼吸暫停綜合征患者軟腭組織中的表達及其意義[D];山東大學;2016年

2 尹雪;原兒茶酸對睡眠呼吸暫停模式慢性間歇性低氧誘導的神經(jīng)認知損傷的保護作用[D];濱州醫(yī)學院;2015年

3 常悅;丹參酮ⅡA對慢性間歇性低氧所致高血壓大鼠心肌ET-1及其受體的影響[D];河北醫(yī)科大學;2016年

4 王翼;慢性間歇性低氧和高脂飼養(yǎng)對兔胰島細胞凋亡的影響研究[D];中南大學;2007年

，

本文編號：1756369