本文選題：GK + 大鼠��； 參考：《昆明醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的： 檢測(cè)Phosducin基因在不同病程糖尿病大鼠模型視網(wǎng)膜組織上的表達(dá)及定位,探討其在糖尿病大鼠視網(wǎng)膜病變過(guò)程中表達(dá)變化及作用,為進(jìn)一步深入研究糖尿病視網(wǎng)膜病變的發(fā)病機(jī)制提供實(shí)驗(yàn)依據(jù)。 方法： 選取實(shí)驗(yàn)組糖尿病GK大鼠32只和對(duì)照組Wistar大鼠32只,按糖尿病病程分為四組,即糖尿病4W組、8W組、16W組、24W組,每組8只。對(duì)照組與實(shí)驗(yàn)組周齡相對(duì)應(yīng)分組,每組8只。采用熒光素眼底血管造影(fundus fluorescein angiography, FFA)技術(shù),分析大鼠模型視網(wǎng)膜病變程度與病程的關(guān)系。采用實(shí)時(shí)熒光定量多聚酶鏈反應(yīng)(RT-real-time PCR)技術(shù),分析Phosducin基因在不同病程糖尿病大鼠視網(wǎng)膜組織上的表達(dá)量,并應(yīng)用免疫組化技術(shù)檢測(cè)Phosducin基因在糖尿病大鼠視網(wǎng)膜組織上的表達(dá)定位。 結(jié)果： 1、FFA檢查結(jié)果顯示,GK大鼠DR的發(fā)生并不完全與糖尿病病程及血糖高低成正相關(guān)。 2、RT-PCR結(jié)果顯示,Phosducin在對(duì)照組大鼠各期視網(wǎng)膜的表達(dá)變化無(wú)明顯差異(P0.05)。與對(duì)照組相比,糖尿病組各期視網(wǎng)膜其表達(dá)是下降的,且隨著周齡的增加,表達(dá)越來(lái)越低,即4W時(shí)開(kāi)始降低,24W時(shí)降到最低(P0.01)。 3、免疫組化結(jié)果顯示Phosducin表達(dá)在視椎視桿層和外核層細(xì)胞,陽(yáng)性細(xì)胞在對(duì)照組大鼠各期無(wú)明顯差異(P0.05)。與對(duì)照組相比,糖尿病組各期視網(wǎng)膜其陽(yáng)性表達(dá)是下降的,且隨著周齡的增加,表達(dá)逐漸降低,以內(nèi)核層變化最為明顯,即4W時(shí)開(kāi)始降低,24W時(shí)降到最低(P0.01)。 結(jié)論： 1、糖尿病GK大鼠DR的發(fā)生并不完全與糖尿病病程及血糖高低成正相關(guān)。 2、Phosducin基因是視覺(jué)適應(yīng)的重要調(diào)控因子,在視覺(jué)傳導(dǎo)通路中起重要的調(diào)節(jié)作用,可能與神經(jīng)元細(xì)胞功能障礙有關(guān),其表達(dá)與糖尿病的病程密切相關(guān)。 3、Phosducin基因表達(dá)異常變化,可能是糖尿病早期視功能障礙的原因之一。
[Abstract]:Objective:To detect the expression and localization of Phosducin gene in the retina of diabetic rats with different course of disease, and to explore the changes and roles of Phosducin gene expression in the process of diabetic retinopathy.To provide experimental evidence for further study of the pathogenesis of diabetic retinopathy.Methods:Thirty-two diabetic GK rats in the experimental group and 32 Wistar rats in the control group were divided into four groups according to the course of diabetes.The control group and the experimental group were divided into groups of 8 rats in each group.Fundus fluorescein angiographic (FFAA) technique was used to analyze the relationship between the degree of retinopathy and the course of retinopathy in rats with fundus fluorescein angiography (FFA).The expression of Phosducin gene in the retina of diabetic rats with different course of disease was analyzed by real-time real-time PCR and the localization of Phosducin gene expression in the retina of diabetic rats was detected by immunohistochemical technique.Results:The results of FFA showed that Dr in GK rats was not positively correlated with the course of diabetes and the level of blood sugar.2the results of RT-PCR showed that there was no significant difference in the expression of Phosducin in the retina of rats in the control group.Compared with the control group, the expression of retina decreased in each stage of diabetic group, and with the increase of week age, the expression became lower and lower, that is, at 4 W, the expression began to decrease to the lowest level (P 0.01) at 24 W.3Immunohistochemical results showed that Phosducin was expressed in the cells of the optic rod layer and the outer nuclear layer of the optic vertebrae, and there was no significant difference in the positive cells between the control group and the control group (P 0.05).Conclusion:1. The incidence of Dr in diabetic GK rats was not positively correlated with the course of diabetes and the level of blood glucose.2 Phosducin gene is an important regulatory factor for visual adaptation, which plays an important regulatory role in the visual conduction pathway and may be related to neuronal dysfunction, and its expression is closely related to the course of diabetes mellitus.3 abnormal expression of Phosducin gene may be one of the causes of visual dysfunction in the early stage of diabetes mellitus.
【學(xué)位授予單位】：昆明醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R774.1

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