發(fā)布時間：2018-03-21 01:06

  本文選題：K多糖　切入點：糖尿病視網(wǎng)膜病變　出處：《眼科新進展》2017年11期 　論文類型：期刊論文

【摘要】：目的探究大腸桿菌K5多糖對糖尿病視網(wǎng)膜病變小鼠視網(wǎng)膜新生血管形成的影響。方法發(fā)酵培養(yǎng)大腸桿菌K5菌株,對培養(yǎng)液進行分離純化制備K5多糖,并分析K5多糖的純度;雄性ICR小鼠被隨機分為正常組、模型組和干預(yù)組,其中正常組是健康小鼠,而模型組和干預(yù)組均使用鏈脲佐菌素建立糖尿病小鼠模型,干預(yù)組于造模后使用K5多糖干預(yù)4周,造模后8周各組取小鼠眼球組織,行HE染色及視網(wǎng)膜鋪片ADPase染色;采用Real-time PCR和Western blotting檢測各組小鼠眼球組織血管內(nèi)皮生長因子(vascular endothelial growth factor,VEGF)及基質(zhì)金屬蛋白酶-2(matrix metalloproteinase,MMP-2)的表達情況。結(jié)果 HPLC結(jié)果顯示制備的K5多糖純度在95%以上;HE染色及ADPase染色結(jié)果顯示正常組小鼠視網(wǎng)膜只見少量血管,模型組小鼠視網(wǎng)膜血管數(shù)目顯著高于正常組,而干預(yù)組小鼠視網(wǎng)膜血管數(shù)目較模型組顯著下降(P0.05);Real-time PCR和Western blot結(jié)果顯示,模型組小鼠眼球組織VEGF和MMP-2的mRNA及蛋白表達水平顯著高于正常組(均為P0.05),而干預(yù)組VEGF和MMP-2的mRNA及蛋白表達水平顯著低于模型組(均為P0.05)。結(jié)論大腸桿菌K5多糖能夠抑制糖尿病視網(wǎng)膜病變小鼠視網(wǎng)膜新生血管形成,其作用機制可能與抑制VEGF及MMP-2的表達有關(guān)。
[Abstract]:Objective to investigate the effect of Escherichia coli K5 polysaccharide on retinal neovascularization in diabetic retinopathy mice. Male ICR mice were randomly divided into normal group, model group and intervention group. The normal group was healthy mice, while the model group and intervention group were all used streptozotocin to establish diabetic mice model, the intervention group was treated with K5 polysaccharide for 4 weeks. After 8 weeks, the eyeball tissues of each group were taken and stained by HE staining and ADPase staining of retina. Real-time PCR and Western blotting were used to detect the expression of vascular endothelial growth factor (VEGF), vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP-2) in the eyeball of mice. Results the results of HPLC showed that the purity of K5 polysaccharide was more than 95% and ADPase was more than 95%. The staining results showed that there were only a few blood vessels in the retina of normal mice. The number of retinal vessels in the model group was significantly higher than that in the normal group, while the number of retinal vessels in the intervention group was significantly lower than that in the model group. The results of real-time PCR and Western blot showed that. The mRNA and protein expression levels of VEGF and MMP-2 in the model group were significantly higher than those in the normal group (both P0.05A), while the mRNA and protein expression levels of VEGF and MMP-2 in the intervention group were significantly lower than those in the model group (both P0.050.Conclusion the expression of Escherichia coli K5 polysaccharides can be observed in the model group. Inhibition of retinal neovascularization in diabetic retinopathy mice. The mechanism may be related to the inhibition of the expression of VEGF and MMP-2.
【作者單位】： 安陽市眼科醫(yī)院;
【分類號】：R587.2;R774.1
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本文編號：1641589