本文選題：GFP小鼠　切入點(diǎn)：YFP小鼠　出處：《眼科新進(jìn)展》2017年08期 　論文類型：期刊論文

【摘要】：目的建立單側(cè)眼視神經(jīng)軸索鈍性損傷模型,觀察視神經(jīng)軸突退行性病變過程和小膠質(zhì)細(xì)胞的變化。方法將標(biāo)記神經(jīng)軸突的YFP小鼠和標(biāo)記小膠質(zhì)細(xì)胞的GFP小鼠分為手術(shù)組和正常對照組,并于視神經(jīng)軸索鈍性損傷手術(shù)后4 h、1 d、3 d、5 d、10 d分離視神經(jīng),以激光共聚焦顯微鏡觀察神經(jīng)軸突受損程度及小膠質(zhì)細(xì)胞的變化。結(jié)果與對正常對照組比較,YFP小鼠術(shù)后4 h損傷處視神經(jīng)軸突斷裂;術(shù)后1 d視神經(jīng)軸突開始出現(xiàn)念珠化;術(shù)后3 d視神經(jīng)軸突大部分念珠化;術(shù)后5 d視神經(jīng)軸突開始從念珠狀轉(zhuǎn)變?yōu)樗槠瑺?術(shù)后10 d視神經(jīng)軸突形成大量碎片。GFP小鼠與正常對照組相比,術(shù)后4 h形成膠質(zhì)瘢痕,靜息態(tài)小膠質(zhì)細(xì)胞開始大量出現(xiàn);術(shù)后1 d激活態(tài)小膠質(zhì)細(xì)胞大量增多并開始覆蓋受損區(qū)域;術(shù)后3 d大量的激活態(tài)小膠質(zhì)細(xì)胞基本覆蓋了受損區(qū)域;術(shù)后5 d、10 d雖然視神經(jīng)的退行性病變持續(xù)惡化,但是小膠質(zhì)細(xì)胞的數(shù)量基本保持穩(wěn)定。結(jié)論小鼠視神經(jīng)受損后軸突發(fā)生不可逆的退行性病變,同時(shí)并伴隨著小膠質(zhì)細(xì)胞的激活和增多,說明小膠質(zhì)細(xì)胞與視神經(jīng)的退行性病變密切關(guān)聯(lián)。
[Abstract]:Objective to establish a model of unilateral optic nerve axonal blunt injury. To observe the process of optic nerve axonal degeneration and the changes of microglia. Methods YFP mice labeled with nerve axons and GFP mice labeled with microglia were divided into operation group and normal control group. The optic nerve was separated from the optic nerve at 4 h, 1 day, 3 days, 5 days and 10 days after the operation of blunt axonal injury of optic nerve. Results compared with the normal control group, the optic nerve axons of the YFP mice were damaged 4 hours after operation, and the axons of the optic nerve began to appear rosary on the 1st day after operation, and the damage degree of the axons and the changes of microglia were observed by laser confocal microscope. On the 3rd day after operation, most of the axons of the optic nerve became beads, 5 days after the operation, the axons of the optic nerve began to change from the beads to the fragments, and on the 10th day after the operation, a large number of fragments were formed in the optic nerve axons. Compared with the normal control group, the glial scar was formed in the GFP mice at 4 hours after operation. Resting microglia began to appear in large numbers, activated microglia increased a lot and began to cover the damaged area on the 1st day after operation, and a large number of activated microglia covered the damaged area on the 3rd day after operation. Although the degenerative lesion of optic nerve continued to deteriorate on the 5th day after operation, the number of microglia remained stable. Conclusion there is irreversible degeneration of the axon after optic nerve injury in mice. At the same time, the activation and increase of microglia showed that microglia were closely related to the degeneration of optic nerve.
【作者單位】： 蘭州大學(xué);
【基金】：國家自然科學(xué)基金資助(編號:31471953)~~
【分類號】：R774.6

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