本文關(guān)鍵詞： 角膜 新生血管 Akt　出處：《中南大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：目的觀察大鼠角膜堿燒傷新生血管模型中蛋白激酶B(Akt)的表達,探討Akt在角膜新生血管形成過程中的作用。 方法將20只成年大鼠分為正常對照組A組(5只5眼)及實驗組(15只15眼),實驗組按角膜堿燒傷后的不同時間分為B、C、D三組,每組各5只5眼。用浸泡1%NaOH溶液的直徑3mm大小圓形濾紙貼敷于實驗組大鼠的右眼角膜中央40s,B組為角膜堿燒傷后3d,C組為角膜堿燒傷后5d,D組為角膜堿燒傷后14d。自角膜堿燒傷24小時后開始每日行裂隙燈檢查,觀察各組角膜新生血管生長情況,并用western印跡法檢測各組角膜組織中Akt表達的變化。 結(jié)果1.在大鼠角膜堿燒傷后24小時,角鞏緣血管開始擴張,48小時角鞏緣處開始有血管向透明角膜生長,第14天角膜遍布新生血管,血管長而稀疏,角膜中央變薄。2.在大鼠角膜堿燒傷新生血管模型中,C組Akt蛋白表達水平最高,為0.840±0.015,與其他各組相比Akt蛋白表達差異具有統(tǒng)計學(xué)意義(P0.05),各實驗組與正常對照組相比Akt蛋白表達差異具有統(tǒng)計學(xué)意義(P0.05)。 結(jié)論1.在大鼠角膜堿燒傷后一定時間內(nèi),Akt的表達增強并呈上升趨勢。2.Akt在大鼠角膜堿燒傷新生血管形成過程中可能具有促進作用。
[Abstract]:Objective To observe the expression of protein kinase B (Akt) in the neovascularization model of rat corneal alkali burn, and to explore the role of Akt in the formation of corneal neovascularization.
Methods 20 adult rats were divided into normal control group and A group (5 eyes of 5) and experimental group (15 eyes of 15), the experimental group at different time after corneal alkali burn were divided into B, C, D three groups, 5 rats in each group. 5 eyes with 3mm diameter size of circular filter paper soaked 1%NaOH solution is applied to the experimental group rats right central corneal 40s, B group after corneal alkali burn 3D group C after corneal alkali burn 5D group D after corneal alkali burn 14D. corneal alkali burn for 24 hours after the daily slit lamp examination, to observe the growth of corneal neovascularization. Change and Western blotting to detect the expression of Akt in corneal tissue.
Results 1. corneal alkali burn in rats after 24 hours, the limbus vessels began to expand, 48 hours perilimbal began to clear corneal vascular growth, fourteenth days in corneal neovascularization, vascular and sparse, central corneal thinning.2. in corneal neovascularization after corneal alkali burn model of rats in C group, Akt the expression of the highest level, 0.840 + 0.015, compared with the other groups were statistically significant differences in expression of Akt protein (P0.05), the experimental group compared with the normal control group was statistically significant difference in Akt protein expression (P0.05).
Conclusion 1., the expression of Akt increased and increased in a certain time after corneal alkali burn in rats..2.Akt may play a role in promoting angiogenesis in rat corneal alkali burn.

【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R772.2

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本文編號：1525700