本文關鍵詞： 慶大霉素 川芎嗪 耳毒性 抗氧化 SOD SOD SOD　出處：《中華耳科學雜志》2016年03期 　論文類型：期刊論文

【摘要】：目的研究川芎嗪(tetraethylplyrazine,TMP)對慶大霉素(Gentamycin,GM)耳蝸毒性的抗氧化作用并探討該機制與銅鋅超氧化物歧化酶(Cu/Zn superoxide dismutase,Cu/Zn SOD或SOD1)、錳超氧化物歧化酶(Mn-superox-ide dismutase,Mn SOD或SOD2)及細胞外超氧化物歧化酶(ECSOD或SOD3)蛋白表達的關系。方法將60只聽力正常成年豚鼠隨機分為4組,即GM組、TMP組、GM+TMP組、對照組。GM組(15只)肌注硫酸慶大霉素120mg·kg-1·d-1,連續(xù)12天;TMP組(15只)腹腔注射鹽酸川芎嗪30mg·kg-1·d-1,連續(xù)12天;GM+TMP組(15只)肌注硫酸慶大霉素120mg·kg-1·d-1及腹腔注射鹽酸川芎嗪30mg·kg-1·d-1,連續(xù)12天;對照組(15只)腹腔注射與GM組等量的生理鹽水2.5ml·kg-1·d-1,連續(xù)12天。每組豚鼠首次用藥前及末次用藥后第一天均行ABR檢測。實驗結束后將豚鼠斷頭處死并取耳蝸標本,免疫組化法對其細胞內SOD1、SOD2、SOD3蛋白表達進行定位研究。結果用藥后GM組聽力閾值明顯升高,與正常組、GM+TMP組比較有明顯差異(P0.01),正常對照組與TMP組聽力閾值用藥前后相比無顯著性差異(P0.05)。耳蝸螺旋神經節(jié)處模型組和拮抗組SOD1、SOD2、SOD3陽性蛋白表達無統(tǒng)計學差異(P0.05),毛細胞處拮抗組SOD1、SOD2陽性蛋白表達較模型組明顯(P0.05),SOD3陽性蛋白表達無統(tǒng)計學差異(P0.05)。結論慶大霉素具有耳毒性,可導致豚鼠耳蝸毛細胞損傷,川芎嗪可通過對毛細胞處SOD1、SOD2的蛋白表達調控機制來實現(xiàn)抗氧化防護作用。
[Abstract]:Objective to study the antioxidation effect of tetraethylplyrazine#en0# (TMP) on the cochlear toxicity of gentamicin Gentamycin (GM) and its relationship with Cu / Zn superoxide dismutase Cu / Zn SOD or SOD1, Mn-superoxide dismutase Mn-superox-ide dismutase mn SOD or SOD2 and extracellular superoxide dismutase (SOD). Methods 60 adult guinea pigs with normal hearing were randomly divided into 4 groups. That is, GM TMP group, TMP group, GM TMP group, Group GM (n = 15) received gentamicin sulfate 120mg 路kg-1 路d-1 intraperitoneally for 12 days (n = 15) ligustrazine hydrochloride (30 mg 路kg-1 路d -1) and GM TMP group (n = 15 for 12 days)) gentamicin sulfate (120 mg 路kg-1 路d -1) and ligustrazine hydrochloride (30 mg 路kg-1 路d -1 intraperitoneally for 12 days). 15 rats in the control group were injected intraperitoneally with 2.5 ml 路kg-1 路d -1 of normal saline as in GM group for 12 days. ABR was detected in each group before and on the first day after the last administration. After the experiment, the guinea pigs were killed and cochlear specimens were taken. Immunohistochemical method was used to study the expression of SOD _ 1, so _ (2) and so _ (3) protein in the cells. Results the hearing threshold of GM group was significantly higher than that of control group. There was significant difference between GM TMP group and normal control group (P 0.01), but there was no significant difference in hearing threshold between normal control group and TMP group (P 0.05). There was no significant difference in the expression of SOD 1, so D 2 and so D 3 positive protein in cochlear spiral ganglion model group and antagonistic group. There was no significant difference in the expression of SOD1 and SOD2 between the two groups. Conclusion Gentamycin has ototoxicity. Tetramethylpyrazine can regulate the protein expression of SOD1 and SOD2 in hair cells to protect against oxidation.
【作者單位】： 寧波衛(wèi)生職業(yè)技術學院;浙江中醫(yī)藥大學;浙江大學醫(yī)學院附屬兒童醫(yī)院;
【分類號】：R764
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本文編號：1524951