本文關(guān)鍵詞： 翼狀胬肉 TGF-β1 VEGF　出處：《蘇州大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:通過研宄初發(fā)及復(fù)發(fā)性翼狀胬肉組織中組織轉(zhuǎn)化因子β1(TGF-β1)及血管內(nèi)皮生長(zhǎng)因子(VEGF)的表達(dá)情況,以及二者之間的相關(guān)性,本實(shí)驗(yàn)旨在探討TGF-β1、VEGF在翼狀胬肉的發(fā)病及復(fù)發(fā)過程中的作用。方法:本實(shí)驗(yàn)收集了翼狀胬肉患者30例,其中初發(fā)者21例、復(fù)發(fā)者9例以及正常鼻側(cè)球結(jié)膜組織8例。使用蘇木素-伊紅染色(HE染色)法顯微鏡下觀察組織形態(tài),免疫組織化學(xué)法(Elivision法)檢測(cè)TGF-β1及VEGF翼狀胬肉組織和正常球結(jié)膜組織中的表達(dá)情況。比較兩因子在翼狀胬肉組與正常結(jié)膜組的表達(dá)是否有顯著性差異;在此基礎(chǔ)上進(jìn)一步比較這兩個(gè)因子在初發(fā)性翼狀胬肉組及復(fù)發(fā)性翼狀胬肉組是否存在顯著性差異,并分析TGF-β1及VEGF表達(dá)的相關(guān)性。結(jié)果:組織學(xué)觀察顯示翼狀胬肉組織上皮層及上皮下組織均可見纖維組織增生,新生血管豐富,周圍伴炎性細(xì)胞。免疫組織化學(xué)結(jié)果顯示:TGF-β1、VEGF在翼狀胬肉有明顯的陽(yáng)性表達(dá),而在正常球結(jié)膜組織僅有少量表達(dá)。30例翼狀胬肉組織中,TGF-β1、VEGF陽(yáng)性表達(dá)率分別為63.33%、70.00%;而在8例正常結(jié)膜組織中的表達(dá)率僅為25.00%,12.50%。TGF-β1在翼狀胬肉初發(fā)組和復(fù)發(fā)組的陽(yáng)性表達(dá)率分別為57.14%,88.89%,VEGF在翼狀胬肉初發(fā)組和復(fù)發(fā)組的陽(yáng)性表達(dá)率分別為57.14%,100%,均有差異性(P0.05)。統(tǒng)計(jì)結(jié)果顯示:在翼狀胬肉組和復(fù)發(fā)性翼狀胬肉中TGF-β1、VEGF的表達(dá)呈正相關(guān)(P0.05)。結(jié)論:TGF-β1、VEGF在翼狀胬肉中均為高表達(dá),在復(fù)發(fā)性病例中此種高表達(dá)更顯著。TGF-β1、VEGF復(fù)發(fā)性翼狀胬肉組織中的表達(dá)存在明顯相關(guān)性,提示它們?cè)谝頎铈廊獾陌l(fā)病及特別是復(fù)發(fā)過程中存在起重要作用。
[Abstract]:Objective: to study the expression of tissue transforming factor 尾 1 (TGF- 尾 1) and vascular endothelial growth factor (VEGF) in primary and recurrent pterygium and the correlation between them. The purpose of this study was to investigate the role of TGF- 尾 _ 1 VEGF in the pathogenesis and recurrence of pterygium. Methods: thirty patients with pterygium were collected, among whom 21 were primary pterygium patients. 9 cases of recurrence and 8 cases of normal nasal bulbar conjunctiva were observed under microscope with hematoxylin eosin staining and HE staining. The expression of TGF- 尾 1 and VEGF in pterygium and normal bulbar conjunctiva was detected by immunohistochemistry. On this basis, the difference between the two factors in the primary pterygium group and the recurrent pterygium group was further compared. The correlation between TGF- 尾 1 and VEGF expression was analyzed. Results: histopathological observation showed that fibrous tissue proliferated and neovascularization was abundant in the epithelium and subcutaneous tissue of pterygium. Peripheral inflammatory cells. Immunohistochemical results showed that VTGF- 尾 1 + VEGF was positively expressed in pterygium. The positive expression rate of TGF- 尾 1 in pterygium was 63.3370.00in normal bulbar conjunctiva and 25.000.50. TGF- 尾 1 in primary pterygium and recrudescent pterygium respectively, and the positive expression rate of TGF- 尾 1 in 8 cases of normal conjunctiva was 25.000.12.50. TGF- 尾 1 was expressed in primary pterygium and recurrent pterygium. The positive expression rates of VEGF in the primary pterygium group and the recurrent pterygium group were 57.14 and 100, respectively. The statistical results showed that the expression of TGF- 尾 _ 1VEGF in pterygium and recurrent pterygium was positively correlated with that in pterygium. Conclusion the expression of TGF- 尾 _ 1VEGF in pterygium is positively correlated with that of recurrent pterygium. Conclusion the expression of TGF- 尾 _ 1 VEGF in pterygium is positively correlated with that in recurrent pterygium. All of them were highly expressed in meat. The overexpression of VEGF in recurrent pterygium was significantly correlated with that in recurrent pterygium, suggesting that they play an important role in the pathogenesis of pterygium, especially in the recurrence of pterygium.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R777.33

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