本文關(guān)鍵詞： 抑癌基因 整合素連接激酶 鱗狀細胞癌 眼瞼結(jié)膜 免疫組織化學(xué)　出處：《安徽醫(yī)科大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：研究整合素連接激酶(integrin-linked kinase, ILK)和抑癌基因PTEN(10號染色體上缺失與張力蛋白同源的磷酸酶基因)在眼瞼結(jié)膜鱗狀細胞癌中的表達及其與眼瞼結(jié)膜鱗狀細胞癌的分化程度的關(guān)系和相關(guān)性,探討它們在眼瞼結(jié)膜鱗狀細胞癌發(fā)生發(fā)展中的作用和臨床意義。 方法：1、收集手術(shù)切除的34例眼瞼結(jié)膜鱗狀細胞癌標(biāo)本的癌組織、10例相應(yīng)癌旁正常眼瞼結(jié)膜組織,經(jīng)4%多聚甲醛固定后,常規(guī)脫水,石蠟包埋。 2、應(yīng)用免疫組織化學(xué)EliVisionTMplus法檢測PTEN蛋白和ILK蛋白在眼瞼結(jié)膜鱗狀細胞組織、相應(yīng)癌旁正常眼瞼結(jié)膜組織中的表達情況。 結(jié)果：1、PTEN蛋白在眼瞼結(jié)膜鱗狀細胞癌組織、癌旁正常眼瞼結(jié)膜組織中的表達陽性率分別為23.53%(8/34),100.00%(10/10),PTEN蛋白在眼瞼結(jié)膜鱗狀細胞癌組織的表達明顯低于癌旁正常眼瞼結(jié)膜組織,差別有顯著性(P=0.000,P0.01)。ILK蛋白在眼瞼結(jié)膜鱗狀細胞癌組織、癌旁正常眼瞼結(jié)膜組織中的表達陽性率分別為73.53%(25/34),0.00%(0/10),ILK蛋白在眼瞼結(jié)膜鱗狀細胞癌組織的表達明顯高于癌旁正常眼瞼結(jié)膜組織,差別有顯著性(P=0.000,P0.01)。 2、PTEN蛋白和ILK蛋白的表達與患者的年齡、性別無顯著性差別(P0.05)。ILK在眼瞼結(jié)膜鱗狀細胞癌23例高-中分化組中,有14例表達陽性,在11例低分化組中全部表達陽性,兩者間有顯著性差別(P=0.017,P0.05)。PTEN在眼瞼結(jié)膜鱗狀細胞癌23例高-中分化組中,有8例表達陽性,在11例低分化組中全部表達陰性,兩者間有顯著性差別(P=0.034,P0.05)。 3、34例眼瞼結(jié)膜鱗狀細胞癌組織中,PTEN蛋白表達陽性者8例,陰性者26例。ILK蛋白表達陽性者25例,陰性者9例。PTEN蛋白和ILK蛋白表達都呈陽性的3例,都呈陰性的6例；PTEN蛋白表達陰性的26例中,ILK蛋白有19例陽性,ILK蛋白表達陰性的10例中,PTEN蛋白有7例陽性。經(jīng)Spearman等級相關(guān)分析,兩者表達具有負相關(guān)性(r=-0.394,P=0.021,P0.05)。 結(jié)論：1、PTEN(?)白在癌旁正常眼瞼結(jié)膜組織中高表達,在眼瞼結(jié)膜鱗狀細胞癌中陽性表達率顯著減低。PTEN蛋白表達與腫瘤的分化程度相關(guān),提示PTEN蛋白低表達或缺失與眼瞼結(jié)膜鱗狀細胞癌的發(fā)生、發(fā)展有關(guān)。 2、檢測ILK在眼瞼結(jié)膜鱗狀細胞癌組織中的表達明顯高于癌旁正常眼瞼結(jié)膜組織,提示ILK蛋白的表達可能與眼瞼結(jié)膜鱗狀細胞癌的發(fā)生有關(guān)：ILK表達與腫瘤分化程度密切相關(guān),提示ILK可能與眼瞼結(jié)膜鱗狀細胞癌的發(fā)展及惡性潛能有關(guān)。 3、ILK蛋白的表達上調(diào)可能抑制PTEN的活性,PTEN蛋白的表達缺失可能促進ILK蛋白的表達,二者共同參與了眼瞼結(jié)膜鱗狀細胞癌的發(fā)生發(fā)展。ILK基因有可能成為治療眼瞼結(jié)膜鱗狀細胞癌的新靶點。
[Abstract]:Objective: to study integrin-linked kinase of integrin-linked kinase. ILK) and tumor suppressor gene PTEN (phosphatase gene homologous to tensin on chromosome 10). The relationship between the expression and the differentiation degree of squamous cell carcinoma of eyelid conjunctiva in eyelid conjunctiva squamous cell carcinoma. To investigate their role and clinical significance in the development of squamous cell carcinoma of eyelid conjunctiva. Methods:. 1. 10 cases of normal eyelid conjunctival conjunctiva conjunctiva tissue adjacent to carcinoma were collected from 34 cases of eyelid conjunctival squamous cell carcinoma. After fixed with 4% paraformaldehyde, conventional dehydration and paraffin embedding were performed. 2Immunohistochemical EliVisionTMplus method was used to detect PTEN protein and ILK protein in squamous cell of eyelid conjunctiva. Expression of normal eyelid conjunctival tissue adjacent to cancer. Results the positive rates of PTEN protein in the conjunctival squamous cell carcinoma of the eyelid and the adjacent normal eyelid conjunctiva were 23.53 and 8 / 34, respectively. The expression of PTEN protein in squamous cell carcinoma of eyelid conjunctiva was significantly lower than that in adjacent normal eyelid conjunctiva tissue (P 0.000). The positive expression rates of P0.01- ilk protein in conjunctival squamous cell carcinoma of eyelid and adjacent normal eyelid conjunctiva were 73.53 / 25 / 34 respectively. The expression of ilk protein in squamous cell carcinoma of eyelid conjunctiva was significantly higher than that in normal conjunctiva conjunctiva tissue adjacent to carcinoma, and the difference was significant. 2the expression of PTEN protein and ILK protein was not significantly different from that of age and sex. Ilk was found in 23 cases of well-differentiated conjunctival squamous cell carcinoma of eyelid. There were 14 cases of positive expression, all of which were positive in 11 cases of low differentiation group. There was a significant difference between the two groups (P < 0.017). In 23 cases of highly differentiated conjunctival squamous cell carcinoma of eyelid, 8 cases were positive, and all the cases were negative in 11 cases of low differentiation group. There was significant difference between the two groups. 3There were 8 cases of positive expression of PTEN protein in 34 cases of squamous cell carcinoma of eyelid conjunctiva, and 25 cases of positive expression of ilk protein in 26 cases of negative cases. The expression of PTEN protein and ILK protein was positive in 3 cases and negative in 6 cases in 9 cases. Of the 26 cases with negative expression of PTEN protein, 19 cases were positive for ilk protein expression, and 10 cases were positive for ilk protein expression. The expression of PTEN protein in 7 cases was positive, and Spearman grade correlation analysis showed that there was a negative correlation between them. Conclusion: 1: 1 PTEN? The expression of white in the adjacent normal eyelid conjunctiva tissue was higher than that in the squamous cell carcinoma of eyelid conjunctiva. The expression of PTEN protein was correlated with the degree of tumor differentiation. The results suggest that the low expression or absence of PTEN protein is associated with the development of squamous cell carcinoma of the eyelid conjunctiva. 2. The expression of ILK in the squamous cell carcinoma of eyelid conjunctiva was significantly higher than that in the adjacent normal eyelid conjunctival tissue. The results suggest that the expression of ILK protein may be related to the development of squamous cell carcinoma of eyelid conjunctiva. The results suggest that ILK may be related to the development and malignant potential of squamous cell carcinoma of eyelid conjunctiva. 3upregulation of ilk protein may inhibit the activity of PTEN and the deletion of PTEN protein may promote the expression of ILK protein. ILK gene may be a new target for the treatment of eyelid conjunctival squamous cell carcinoma.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R739.7

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