本文關(guān)鍵詞： 喉鱗狀細(xì)胞癌 CXCL13 CXCR5 免疫組織化學(xué)法　出處：《山西醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:觀察趨化因子CXCL13及其受體CXCR5在喉鱗狀細(xì)胞癌組織及癌旁組織中的表達(dá)情況,并分析其與臨床病理參數(shù)(包括性別、年齡、原發(fā)部位、分化程度、TNM分期、淋巴結(jié)轉(zhuǎn)移與否、臨床分期)及預(yù)后之間的相關(guān)性,從而探討其臨床意義。材料與方法:收集2000年2月——2015年6月期間于山西醫(yī)科大學(xué)第一附屬醫(yī)院行喉鱗狀細(xì)胞癌手術(shù)患者的手術(shù)切除組織包埋的石蠟標(biāo)本。選取132例喉鱗癌石蠟組織標(biāo)本及對(duì)應(yīng)66例癌旁正常黏膜石蠟組織標(biāo)本(距腫瘤邊緣5mm的癌旁正常黏膜組織)。選取標(biāo)本病理資料完整,經(jīng)病理診斷均為喉鱗狀細(xì)胞癌,且均為原發(fā),術(shù)前均未接受放療及化療。采用免疫組織化學(xué)法檢測(cè)喉鱗狀細(xì)胞癌和癌旁正常黏膜石蠟包埋組織中CXCL13及CXCR5蛋白的表達(dá)情況。用數(shù)字病理切片掃描儀掃描經(jīng)免疫組化方法處理好的切片,用CaseViewer1.4圖片采集軟件閱片審核,并判定棕色及黃褐色區(qū)域?yàn)殛?yáng)性表達(dá)。采用SPSS Version20.0統(tǒng)計(jì)軟件對(duì)實(shí)驗(yàn)結(jié)果進(jìn)行統(tǒng)計(jì)學(xué)分析。包括χ2檢驗(yàn),Spearman等級(jí)相關(guān)分析及Kaplan-Meier生存分析及COX回歸分析,檢驗(yàn)水準(zhǔn)α=0.05,P≤0.05時(shí)差異具有統(tǒng)計(jì)學(xué)意義。結(jié)果:1、CXCL13及CXCR5在喉鱗癌組織和正常組織中的表達(dá):CXCL13在喉鱗癌組織中陽(yáng)性率為50.8%,癌旁組織中陽(yáng)性率為3.0%;CXCR5在喉鱗癌組織中陽(yáng)性率為45.5%,在癌旁組織中陽(yáng)性率為4.5%,CXCL13及CXCR5在癌和癌旁正常組織中的表達(dá)情況相比存在差異,具有統(tǒng)計(jì)學(xué)意義(P0.05)。2、CXCL13及CXCR5在喉鱗癌組織中的表達(dá)情況與臨床病理參數(shù)的相關(guān)性:CXCL13及CXCR5蛋白表達(dá)強(qiáng)度與性別、年齡無明顯相關(guān)性(P0.05),而與TNM分期、淋巴結(jié)轉(zhuǎn)移情況及臨床分期呈顯著相關(guān)(P0.05);CXCL13及CXCR5的表達(dá)強(qiáng)度在聲門型喉癌組織與聲門上型喉癌組織中具有顯著差異(P0.05);CXCL13的表達(dá)與分化程度相關(guān)(P=0.05)。3、CXCL13與CXCR5在喉鱗癌組織中表達(dá)情況的相關(guān)性及與患者生存時(shí)間的相關(guān)性:在喉鱗癌組織中,CXCL13與CXCR5的表達(dá)具有顯著相關(guān)性;CXCL13及CXCR5低表達(dá)患者與高表達(dá)患者的生存時(shí)間相比差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:CXCL13及CXCR5在喉鱗癌組織中的陽(yáng)性表達(dá)率顯著高于癌旁組織;分析臨床病理參數(shù)與CXCL13及CXCR5表達(dá)水平之間的相關(guān)性發(fā)現(xiàn):CXCL13及其受體CXCR5蛋白的表達(dá)水平可以作為評(píng)估喉鱗癌發(fā)病及可能發(fā)生侵襲、轉(zhuǎn)移的新指標(biāo);通過比較不同表達(dá)水平的喉鱗狀細(xì)胞癌患者的生存時(shí)間,提示CXCL13及CXCR5可能為喉癌患者的不良預(yù)后因子。
[Abstract]:Objective: to observe the expression of chemokine CXCL13 and its receptor CXCR5 in laryngeal squamous cell carcinoma (LSCC) and its adjacent tissues, and to analyze its relationship with clinicopathologic parameters (including sex, age, primary location). The correlation between TNM stage, lymph node metastasis, clinical stage and prognosis. Thus, the clinical significance, materials and methods are discussed. Paraffin wax specimens were collected from patients with laryngeal squamous cell carcinoma (LSCC) surgically performed from February 2000 to June 2015 in the first affiliated Hospital of Shanxi Medical University. 132 cases of laryngeal squamous cell carcinoma (LSCC) were selected as paraffin wax. Tissue specimens and paraffin tissues corresponding to 66 normal mucosa adjacent to cancer (. The normal mucosa adjacent to the tumor was 5 mm from the margin of the tumor. The pathological data of the specimens were complete. All patients were diagnosed as laryngeal squamous cell carcinoma by pathology, and all were primary. The expression of CXCL13 and CXCR5 in paraffin embedded tissues of laryngeal squamous cell carcinoma and adjacent normal mucosa was detected by immunohistochemical method. The expression of CXCL13 and CXCR5 protein in paraffin embedded tissues of laryngeal squamous cell carcinoma and adjacent normal mucosa were detected by digital pathological section. Scanners scan sections processed by immunohistochemistry. The CaseViewer1.4 image acquisition software was used to read the film. SPSS Version20.0 software was used to analyze the results of the experiment, including 蠂 2 test. Spearman grade correlation analysis, Kaplan-Meier survival analysis and COX regression analysis showed that the test level was 0. 05. When P 鈮，

本文編號(hào)：1460911