【摘要】：目的研究甲狀腺術(shù)中甲狀旁腺自體移植過(guò)程中體外臨時(shí)保存對(duì)其細(xì)胞活性和移植后功能的影響,以提高甲狀旁腺自體移植的成活率。方法 (1)以實(shí)驗(yàn)家兔為研究對(duì)象,分為A、B、C 3組,每組8只。取出雙側(cè)下甲狀旁腺,A組立即后續(xù)處理;B、C組分別置于4℃、室溫(22~24℃)生理鹽水中30min。左側(cè)甲狀旁腺統(tǒng)一行HE染色,觀察細(xì)胞形態(tài);右側(cè)甲狀旁腺統(tǒng)一行透射電子顯微鏡檢查,觀察細(xì)胞超微結(jié)構(gòu)變化。(2)實(shí)驗(yàn)家兔分為D、E、F 3組,每組8只,行雙側(cè)甲狀腺全切除術(shù)后,取出雙下甲狀旁腺,D組立即移植(雙側(cè)頸前肌內(nèi),下同);E、F組分別置于4℃、室溫(22~24℃)生理鹽水中30min后移植;3組動(dòng)物于術(shù)前1d,術(shù)后1、3、5、7d抽血監(jiān)測(cè)血鈣、血PTH水平,并于第7天處死動(dòng)物,切取移植的甲狀旁腺組織行HE染色,高倍鏡下觀察甲狀旁腺組織面積及病理?yè)p害。結(jié)果正常甲狀旁腺以主細(xì)胞為主,電鏡下主細(xì)胞核圓、居中;4℃保存后甲狀旁腺細(xì)胞形態(tài)未見(jiàn)明顯變化,主細(xì)胞線粒體輕度腫脹;室溫保存后甲狀旁腺細(xì)胞稍腫脹,部分空泡變性,電鏡下細(xì)胞核形態(tài)不規(guī)則,線粒體極度腫脹變形、嵴斷裂、減少。動(dòng)物雙下甲狀旁腺移植術(shù)后,血鈣、血PTH均明顯下降,之后逐漸上升;術(shù)后7d兩組間差異均有統(tǒng)計(jì)學(xué)意義(P0.05);術(shù)后7dD組:正常甲狀旁腺細(xì)胞密集分布于頸前肌肉組織中;E組部分空泡變性;F組:只有部分健存甲狀旁腺細(xì)胞散在分布于肌肉中。結(jié)論離體甲狀旁腺應(yīng)保存在4℃生理鹽水中,并盡可能在30min內(nèi)完成自體移植。
[Abstract]:Objective to study the effect of temporary preservation on cell activity and post-transplantation function of parathyroid autotransplantation during thyroid surgery in order to improve the survival rate of parathyroid autotransplantation. Methods (1) the rabbits were divided into A, B and C groups with 8 rabbits in each group. Bilateral inferior parathyroid gland was taken out and followed up immediately in group A, and group C was placed in saline at 4 鈩，

本文編號(hào)：2527194