【摘要】：目的觀察不同劑量姜黃素腹腔注射對(duì)脊髓損傷(SCI)大鼠脊髓組織結(jié)構(gòu)及運(yùn)動(dòng)功能的影響。方法將35只SD大鼠隨機(jī)分為姜黃素高劑量組、姜黃素中劑量組、姜黃素低劑量組、SCI組和對(duì)照組。除對(duì)照組外的4組大鼠用HI-0400脊髓打擊器制備SCI模型。姜黃素高、中、低劑量組大鼠SCI后30 min腹腔注射200、100、50 mg/kg姜黃素。對(duì)照組常規(guī)飼養(yǎng)并在相同時(shí)間點(diǎn)腹腔注射等量生理鹽水。SCI后1、3、7 d取各組大鼠脊髓組織行HE染色光鏡下觀察脊髓組織形態(tài)。SCI后7、14、21、28 d行BBB評(píng)分和改良Rivlin斜板實(shí)驗(yàn)評(píng)價(jià)各組大鼠后肢運(yùn)動(dòng)功能。結(jié)果 SCI組大鼠損傷后1 d可以觀察到脊髓局灶性出血,灰質(zhì)和白質(zhì)交界線不清晰;很多神經(jīng)元聚合,胞質(zhì)內(nèi)空泡較多,而且還伴隨炎癥細(xì)胞的浸潤(rùn)。損傷后3 d損傷區(qū)域出血量有所減少,脂肪液化,細(xì)胞核破碎,大量細(xì)胞溶解消失,大多胞核萎縮消失,灰質(zhì)中心明顯壞死,灰質(zhì)內(nèi)軸索紊亂不規(guī)則,胞質(zhì)內(nèi)有更多的空泡形成,細(xì)胞凋亡明顯增多;損傷后7 d大鼠SCI程度有所好轉(zhuǎn),出血現(xiàn)象基本消失,雖然灰質(zhì)、白質(zhì)內(nèi)仍可見少量液化壞死,空泡,細(xì)胞丟失現(xiàn)象有所減輕。姜黃素高、中、低劑量組大鼠脊髓組織形態(tài)較SCI組改善,脊髓神經(jīng)元變性壞死及脊髓組織炎細(xì)胞浸潤(rùn)較輕,神經(jīng)元凋亡的數(shù)目較少。SCI后7、14、21、28 d,SCI組大鼠BBB評(píng)分和Rivlin斜板實(shí)驗(yàn)最大斜板角度明顯低于對(duì)照組(P均0.05)。隨著時(shí)間推移,SCI組和姜黃素高、中、低劑量組大鼠SCI后BBB評(píng)分和Rivlin斜板實(shí)驗(yàn)最大斜板角度逐漸升高(P均0.05)。相同時(shí)點(diǎn)BBB評(píng)分和Rivlin斜板實(shí)驗(yàn)最大斜板角度姜黃素中劑量組小于對(duì)照組(P均0.05),姜黃素低、高劑量組小于姜黃素中劑量組(P均0.05),SCI組小于姜黃素低、高劑量組(P均0.05)。結(jié)論姜黃素腹腔注射可以促進(jìn)SCI大鼠脊髓組織結(jié)構(gòu)及運(yùn)動(dòng)功能的恢復(fù),100 g/kg姜黃素促進(jìn)SCI大鼠脊髓運(yùn)動(dòng)功能恢復(fù)的效果最好。
[Abstract]:Objective to observe the effects of different doses of curcumin on spinal cord tissue structure and motor function in (SCI) rats with spinal cord injury. Methods 35 SD rats were randomly divided into curcumin high dose group, curcumin medium dose group, curcumin low dose group, SCI group and control group. The SCI model was established by HI-0400 spinal cord percussion apparatus in 4 groups except the control group. Rats in high, middle and low dose curcumin groups were intraperitoneally injected with 200, 100, 50 mg/kg curcumin 30 min after SCI. The spinal cord tissue of each group was observed by HE staining on the 7th day after sci. BBB score and modified Rivlin oblique plate test were performed to evaluate the motor function of the hindlimb of the rats in each group on the 7th day after sci. On the 7th, 14th, 21st and 28th day, the motor function of the hindlimb of the rats in each group was evaluated by routine feeding and intraabdominal injection of the same amount of saline at the same time point. Results focal hemorrhage of spinal cord was observed 1 day after injury in SCI group, the boundary between gray matter and white matter was not clear, and many neurons converged, there were more vacuoles in cytoplasm, and the infiltration of inflammatory cells was accompanied by the infiltration of inflammatory cells. On the 3rd day after injury, the amount of bleeding in the injured area decreased, fat liquefaction, nuclear fragmentation, a large number of cells dissolved and disappeared, most of the nucleus atrophy disappeared, the gray matter center obviously died, the axonal disorder in the gray matter was irregular, more vacuoles were formed in the cytoplasm, and the apoptosis was obviously increased. On the 7th day after injury, the degree of SCI was improved and the bleeding phenomenon disappeared basically. Although a small amount of liquefaction necrosis, vacuole and cell loss were found in the gray matter and white matter, the loss of cells was alleviated. Compared with the SCI group, the morphology of spinal cord tissue in the high, middle and low dose groups was improved, the degeneration and necrosis of spinal cord neurons and the infiltration of inflammatory cells in spinal cord tissue were less, and the number of neuronal apoptosis was less. 7 days after sci, 14, 21, 28 days after sci, the BBB score and the maximum oblique plate angle of Rivlin oblique plate test in sci group were significantly lower than those in control group (P 0.05). With the passage of time, the BBB score and the maximum oblique plate angle of SCI group and curcumin group increased gradually after SCI, and the maximum oblique plate angle of Rivlin oblique plate test increased gradually. At the same time, the BBB score and the maximum oblique plate angle of curcumin in the middle dose group were lower than those in the control group (P < 0.05), and the curcumin in the high dose group was lower than that in the curcumin middle dose group (P < 0.05), SCI), and that in the high dose group was lower than that in the high dose group (P < 0.05). Conclusion Intraabdominal injection of curcumin can promote the recovery of spinal cord tissue structure and motor function in SCI rats, and 100 g/kg curcumin has the best effect on the recovery of spinal cord motor function in SCI rats.
【作者單位】： 延安大學(xué)醫(yī)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(81650022,81641048) 陜西省教育廳科研項(xiàng)目(15JK1813)
【分類號(hào)】：R651.2

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 趙子牛,付海霞,林道明;缺血預(yù)處理對(duì)兔缺血損傷脊髓組織中自由基變化的影響[J];溫州醫(yī)學(xué)院學(xué)報(bào);2002年05期

2 趙敏;劉玲;張世強(qiáng);楊天祝;張英澤;潘進(jìn)社;;脊髓組織蛋白質(zhì)組分析中雙向電泳技術(shù)應(yīng)用效果觀察[J];山東醫(yī)藥;2010年22期

3 張衡,高建新,徐桂艷,劉沂訓(xùn);胚胎脊髓組織植入成年鼠損傷脊髓的形態(tài)學(xué)研究[J];山東醫(yī)科大學(xué)學(xué)報(bào);1999年03期

4 梁朝革;賈連順;陳長(zhǎng)青;陳梓峰;;椎體火器傷對(duì)挫傷脊髓組織鐵蛋白表達(dá)的影響[J];創(chuàng)傷外科雜志;2006年06期

5 張屹;趙敏;;微波幅射固定脊髓組織及保存其超微結(jié)構(gòu)[J];廣東解剖學(xué)通報(bào);1991年01期

6 周初松,靳安民,馬大烈;大鼠脊髓組織一氧化氮合酶顯微及超微結(jié)構(gòu)特征[J];第一軍醫(yī)大學(xué)學(xué)報(bào);2000年06期

7 趙剛,廖維宏,蔡錦方,張亞彬;同種胚胎脊髓移植物對(duì)成年大鼠損傷脊髓組織形態(tài)與功能的修復(fù)作用[J];中華器官移植雜志;1997年02期

8 李琦;曾炳芳;徐建廣;孔維清;;人膠質(zhì)源性神經(jīng)生長(zhǎng)因子真核表達(dá)載體構(gòu)建及在大鼠脊髓組織中的表達(dá)[J];中國(guó)修復(fù)重建外科雜志;2007年05期

9 ;大鼠脊髓創(chuàng)傷后脊髓組織中3種內(nèi)源性阿片肽含量的動(dòng)態(tài)變化及意義[J];第二軍醫(yī)大學(xué)學(xué)報(bào);1995年05期

10 張秋林,李明,趙杰,王秋根,侯鐵勝,李珍,張玲妹;強(qiáng)啡肽A致截癱大鼠脊髓組織細(xì)胞外腺苷濃度的變化[J];第二軍醫(yī)大學(xué)學(xué)報(bào);2001年10期

相關(guān)會(huì)議論文 前4條

1 王一健;石元?jiǎng)?;脊髓組織中五種金屬元素含量測(cè)定的研究[A];中國(guó)營(yíng)養(yǎng)學(xué)會(huì)第七屆全國(guó)營(yíng)養(yǎng)學(xué)術(shù)會(huì)議論文摘要匯編[C];1996年

2 沈憶新;;X線照射促進(jìn)損傷脊髓組織修復(fù)的實(shí)驗(yàn)研究[A];蘇州市自然科學(xué)優(yōu)秀學(xué)術(shù)論文匯編(2008-2009)[C];2010年

3 林巍巍;李敏;李奕;李想;楊帆;黃月嬌;王曉冬;;BMSCs和脊髓組織互相作用及機(jī)制的體外初步研究[A];中國(guó)解剖學(xué)會(huì)2012年年會(huì)論文文摘匯編[C];2012年

4 許濤;李新志;郭風(fēng)勁;;磁刺激對(duì)脊髓損傷后脊髓組織c-fos基因表達(dá)的影響[A];中國(guó)康復(fù)醫(yī)學(xué)會(huì)第四屆會(huì)員代表大會(huì)暨第三屆中國(guó)康復(fù)醫(yī)學(xué)學(xué)術(shù)大會(huì)論文匯編[C];2001年

相關(guān)重要報(bào)紙文章 前2條

1 常麗君;德開發(fā)出追蹤神經(jīng)再生新方法[N];科技日?qǐng)?bào);2011年

2 記者 劉向;檢測(cè)瘋牛病肉制品有新法[N];新華每日電訊;2004年

相關(guān)博士學(xué)位論文 前2條

1 孫燕;HMGB1在實(shí)驗(yàn)性自身免疫性腦脊髓炎病程中的作用[D];華中科技大學(xué);2015年

2 樸成東;白介素10對(duì)脊髓缺血再灌注損傷中保護(hù)作用的研究[D];吉林大學(xué);2006年

相關(guān)碩士學(xué)位論文 前9條

1 全墨緣;p-p38MAPK在實(shí)驗(yàn)性自身免疫性腦脊髓炎小鼠發(fā)病不同時(shí)期脊髓組織的表達(dá)及SB203580保護(hù)機(jī)制的研究[D];河北醫(yī)科大學(xué);2015年

2 肖一千;EAE小鼠腦及脊髓組織中IL-17及c-Maf表達(dá)與病程關(guān)系的研究[D];南昌大學(xué);2016年

3 劉志國(guó);脊髓手術(shù)中不同溫度生理鹽水沖洗對(duì)脊髓組織的影響[D];南通大學(xué);2008年

4 鄭蕓鶴;丙烯酰胺對(duì)大鼠脊髓組織蛋白激酶的影響[D];山東大學(xué);2010年

5 韓曉英;TOCP誘發(fā)OPIDN后成年母雞大腦和脊髓組織中細(xì)胞骨架蛋白的改變[D];山東大學(xué);2005年

6 劉佳;萊菔硫烷對(duì)實(shí)驗(yàn)性自身免疫性腦脊髓炎小鼠腦及脊髓組織HO-1及IL-17表達(dá)的影響及影響機(jī)制探討[D];河北醫(yī)科大學(xué);2012年

7 吳芳;濾泡輔助性T細(xì)胞參與實(shí)驗(yàn)性自身免疫性腦脊髓炎的初步研究[D];第四軍醫(yī)大學(xué);2014年

8 張彥寧;從TOCP暴露雞脊髓組織中篩選OPIDN誘發(fā)相關(guān)蛋白[D];大連醫(yī)科大學(xué);2012年

9 呂苗苗;動(dòng)脈注射丙泊酚對(duì)兔脊髓缺血再灌注損傷的保護(hù)作用及其機(jī)制的研究[D];第四軍醫(yī)大學(xué);2009年

，

本文編號(hào)：2507433