【摘要】：目的:探索含“界層結(jié)構(gòu)”仿生一體化骨軟骨支架復(fù)合自體骨髓間充質(zhì)干細(xì)胞(BMSCs)修復(fù)豬膝關(guān)節(jié)骨軟骨缺損的有效性。方法:選用9只小香豬,同一實(shí)驗(yàn)動(dòng)物一側(cè)膝關(guān)節(jié)股骨滑車面分別造含實(shí)驗(yàn)組及無陰性對(duì)照組骨軟骨缺損模型,共6只。隨機(jī)將實(shí)驗(yàn)動(dòng)物分為:含“界層結(jié)構(gòu)”nano-HA/CPPf/PLLA仿生一體化支架+自體BMSCs的實(shí)驗(yàn)組(6只12膝);無“界層結(jié)構(gòu)”CPPf/PLLA支架的陰性對(duì)照組(6只12膝);不做任何處理的單純關(guān)節(jié)骨軟骨缺損為空白對(duì)照組(3只3膝)。于術(shù)后12周、24周進(jìn)行實(shí)驗(yàn)標(biāo)本大體觀察、組織學(xué)觀察、MRI檢測(cè)及組織學(xué)評(píng)分。結(jié)果:大體觀察、組織學(xué)觀察及MRI檢測(cè)示實(shí)驗(yàn)組修復(fù)趨勢(shì)較陰性對(duì)照組和空白對(duì)照組明顯,但是三組均未能完全修復(fù)骨軟骨缺損。組織學(xué)觀察三組軟骨修復(fù)區(qū)均以纖維樣組織修復(fù)為主,軟骨修復(fù)區(qū)僅可見少量軟骨細(xì)胞或無軟骨細(xì)胞出現(xiàn),軟骨下骨區(qū)則未見成骨細(xì)胞出現(xiàn)。以上三組于術(shù)后12周、24周行Pineda組織學(xué)評(píng)分,評(píng)分結(jié)果進(jìn)行統(tǒng)計(jì)分析,組間和組內(nèi)不同時(shí)間點(diǎn)比較差異無統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:含“界層結(jié)構(gòu)”nano-HA/CPPf/PLLA仿生一體化支架復(fù)合自體骨髓間充質(zhì)干細(xì)胞未能明顯修復(fù)豬膝關(guān)節(jié)骨軟骨缺損,修復(fù)組織主要為纖維組織。
[Abstract]:Aim: to explore the effectiveness of biomimetic integrated osteochondral scaffold containing "boundary layer structure" combined with autologous bone marrow mesenchymal stem cells (BMSCs) in repairing osteochondral defects of pig knee joint. Methods: nine Xiaoxiang pigs were selected to establish osteochondral defect models with experimental group and control group with and without negative osteochondral defects on one side of the knee joint of the same experimental animal. The experimental animals were randomly divided into experimental group (n = 6, 12 knees) containing "boundary layer structure" nano-HA/CPPf/PLLA biomimetic integrated stent autologous BMSCs, negative control group without "boundary layer structure" CPPf/PLLA stent (6 cases, 12 knees), and negative control group (6 cases, 12 knees). The simple articular cartilage defects without any treatment were blank control group (3 knees). At 12 weeks and 24 weeks after operation, the gross observation, histology observation, MRI detection and histology score of the experimental specimens were carried out. Results: gross observation, histologic observation and MRI test showed that the repair trend of the experimental group was more obvious than that of the negative control group and the blank control group, but none of the three groups could completely repair the osteochondral defects. Histologically, the cartilage repair area of the three groups was mainly fibroid tissue repair, only a small number of cartilage cells or no cartilage cells were found in the cartilage repair area, and no osteoblasts were found in the subcartilage bone area. The above three groups were treated with Pineda histology score at 12 weeks and 24 weeks after operation, and the results were statistically analyzed. There was no significant difference between the three groups at different time points (P 0.05). Conclusion: the "boundary layer structure" nano-HA/CPPf/PLLA biomimetic integrated scaffold combined with autologous bone marrow mesenchymal stem cells can not significantly repair the osteochondral defects of pig knee joint, and the repair tissue is mainly fiber tissue.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R684.3

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