【摘要】：目的探討富含血小板血漿(platelet rich plasma,PRP)與骨形態(tài)發(fā)生蛋白-4(Bone morphogenetic protein-4,BMP-4)的納米羥基磷灰石(Nano hydroxyapatite,Nano-HA)聯(lián)合髓心減壓修復(fù)兔股骨頭壞死的可行性。方法激素法制備新西蘭大白兔股骨頭壞死模型,取36只隨機(jī)分為A、B、C共3組,各12只。A組為模型組,不進(jìn)行任何處理;B組行單純髓心減壓術(shù);C組髓心減壓后植入富含PRP與BMP-4的Nano-HA。分別于術(shù)后2、4、8周處死大白兔,取股骨頭進(jìn)行組織學(xué)觀察及骨小梁圖像分析,并在第8周測定股骨頭骨密度。并用spss軟件對數(shù)據(jù)進(jìn)行分析。結(jié)果(1)組織學(xué)觀察顯示,從術(shù)后第2周開始C組的成骨能力一直好于A、B組,C組術(shù)后2周時開始出現(xiàn)骨小梁,術(shù)后8周時骨小梁已成熟,骨髓組織形成;B組術(shù)后8周時缺損區(qū)仍然在修復(fù),可見骨髓組織;A組術(shù)后8周時仍無骨組織。(2)骨小梁圖像分析顯示,C組術(shù)后2、4、8周骨小梁所占缺損區(qū)面積比例均高于B組,差異有顯著性(P0.05)。(3)骨密度測定顯示,術(shù)后8周C組的骨密度高于B組及A組,差異有顯著性(P0.05)。結(jié)論P(yáng)RP協(xié)同BMP-4可誘導(dǎo)壞死的股骨頭內(nèi)新生骨的形成,Nano-HA可作為髓心減壓后修復(fù)股骨頭壞死良好的支架材料,PRP協(xié)同BMP-4搭載Nano-HA聯(lián)合髓心減壓能加速兔股骨頭壞死修復(fù)。
[Abstract]:Aim to investigate the feasibility of platelet-rich plasma (platelet rich plasma,PRP and bone morphogenetic protein-4 (Bone morphogenetic protein-4,BMP-4 nano-hydroxyapatite (Nano hydroxyapatite,Nano-HA) combined with medullary decompression in the repair of femoral head necrosis in rabbits. Methods the femoral head necrosis model of New Zealand white rabbits was made by hormone method. 36 rabbits were randomly divided into 3 groups, group A (n = 12), group B (n = 12) without any treatment, group B (n = 12) and group B (n = 12), and group B (n = 12, n = 12). Group C implanted Nano-HA. rich in PRP and BMP-4 after decompression. The rabbits were killed at 2, 4 and 8 weeks after operation. The femoral head was taken for histological observation and trabecular image analysis, and the bone mineral density of the femoral head was measured at the 8th week. Spss software was used to analyze the data. Results (1) histological observation showed that the osteogenic ability of group C was better than that of group A from the second week after operation, group B, group C appeared trabecular bone at 2 weeks after operation, bone trabecular matured at 8 weeks after operation, and bone marrow tissue formed. In group B, the defect area was still repaired at 8 weeks after operation, and bone marrow tissue could be seen. There was no bone tissue in group A at 8 weeks after operation. (2) the ratio of trabecular defect area in group C was higher than that in group B at 2,4,8 weeks after operation, and the difference was significant (P0.05). (3). 8 weeks after operation, bone mineral density in group C was significantly higher than that in group B and A (P0.05). Conclusion PRP combined with BMP-4 can induce the formation of new bone in necrotic femoral head. Nano-HA can be used as a good scaffold for repairing osteonecrosis of femoral head after decompression of medullary heart. PRP and BMP-4 combined with Nano-HA combined with decompression of medulla can accelerate the repair of femoral head necrosis in rabbits.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R681.8

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