【摘要】：目的觀察注射右美托咪定(Dexmedetomidine,DEX)預(yù)處理對(duì)在體大鼠脊髓缺血再灌注損傷(Spinal cord ischemia reperfusion injury,SCIRI)后神經(jīng)元自噬和下肢運(yùn)動(dòng)功能的影響。方法 SD大鼠隨機(jī)分為4組(每組30只):假手術(shù)組(Sham組)、缺血再灌注損傷組(IR組,鞘內(nèi)注射30μL生理鹽水)、Dex組(缺血前3 d鞘內(nèi)注射右美托咪定10μg/30μL)、Dex+ATIP組(缺血前3 d鞘內(nèi)注射10μg右美托咪定+10μg阿替美唑共30μL)。Sham組僅暴露主動(dòng)脈弓而不結(jié)扎,其他各組開(kāi)胸后無(wú)創(chuàng)動(dòng)脈夾夾閉主動(dòng)脈弓14 min后再開(kāi)放,建立SCIRI模型。各組手術(shù)前均于L5-6鞘內(nèi)置管并連續(xù)注射3 d。損傷后48 h內(nèi)每12小時(shí)取5只大鼠,采用Tarlov法評(píng)價(jià)大鼠下肢運(yùn)動(dòng)功能;HE染色觀察和計(jì)數(shù)損傷后48 h脊髓前角神經(jīng)元形態(tài)和數(shù)量;Western blot和RT-PCR法測(cè)定損傷后48 h大鼠脊髓組織中自噬相關(guān)蛋白Beclin1、LC3蛋白及mRNA含量。結(jié)果與Sham組比較,術(shù)后各觀察點(diǎn)IR組下肢運(yùn)動(dòng)功能明顯受損,Tarlov評(píng)分下降,脊髓組織中Beclin1、LC3蛋白及mRNA的表達(dá)均明顯降低(P0.05);損傷前3 d鞘內(nèi)注射10μg DEX(Dex組)可改善缺血再灌注損傷后下肢運(yùn)動(dòng)功能,提高Tarlov評(píng)分,改善脊髓Beclin1、LC3 mRNA蛋白的表達(dá)(P0.05),而Dex+ATIP組與IR組比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。損傷后48 h,HE染色顯示,Sham組脊髓前角神經(jīng)元輪廓清晰,結(jié)構(gòu)完整,無(wú)出血、水腫等異常表現(xiàn);IR組、Dex+ATIP組細(xì)胞質(zhì)中的尼氏體消失,神經(jīng)元胞核出現(xiàn)固縮或溶解,廣泛空泡形成;而Dex組神經(jīng)元形態(tài)明顯規(guī)整,且正常神經(jīng)元數(shù)量增多。結(jié)論鞘內(nèi)注射右美托咪定預(yù)處理通過(guò)上調(diào)Beclin1、LC3蛋白和基因表達(dá),增加神經(jīng)元自噬,改善大鼠缺血再灌注后下肢運(yùn)動(dòng)功能。
[Abstract]:Aim to observe the effects of pretreatment with right metametidine (Dexmedetomidine,DEX) on neuronal autophagy and lower limb motor function after spinal cord ischemia-reperfusion injury (Spinal cord ischemia reperfusion injury,SCIRI) in rats in vivo. Methods SD rats were randomly divided into 4 groups (30 rats in each group): sham operation group (Sham group), ischemia reperfusion injury group (IR group, intrathecal injection of 30 渭 L saline), Dex (intrathecal injection of right metomide 10 渭 g / 30 渭 L) 3 days before ischemia). In Dex ATIP group (intrathecal injection of 10 渭 g right metametrimidine 10 渭 g atimeimazole) 3 days before ischemia, only aortic arch was exposed without ligation in 30 渭 L). Sham group, and non-invasive aortic arch was closed for 14 min after thoracotomy in other groups. The SCIRI model was established by intrathecal injection of 10 渭 g right metometrimidine 10 渭 g atimezole. All the patients in each group were placed in the sheath of L _ 5 ~ 6 before operation and injected continuously for 3 d. Within 48 hours after injury, 5 rats were taken out every 12 hours to evaluate the motor function of the lower extremities by Tarlov method, and the morphology and number of neurons in the anterior horn of spinal cord were observed and counted by HE staining at 48 hours after injury. The contents of Beclin1,LC3 protein and mRNA in spinal cord tissue of 48 h after injury were determined by Western blot and RT-PCR. Results compared with Sham group, the motor function of lower extremities and the expression of Beclin1,LC3 protein and mRNA in spinal cord were significantly decreased in IR group after operation (P0.05). The scores of Tarlov were decreased, and the expression of Beclin1,LC3 protein and mRNA in spinal cord were significantly decreased (P0.05). Intrathecal injection of 10 渭 g DEX (Dex 3 days before injury could improve lower limb motor function, increase Tarlov score and improve the expression of Beclin1,LC3 mRNA protein in spinal cord after ischemia-reperfusion injury (P0.05). There was no significant difference between Dex ATIP group and IR group (P0.05). 48 hours after injury, HE staining showed that neurons in the anterior horn of the spinal cord in the Sham group were clear in outline, complete in structure, without hemorrhage and edema. In IR group, the Nissl body disappeared in the cytoplasm of, Dex ATIP group, the nucleus of neurons became pyknotic or dissolved, and extensive vacuoles were formed, while the morphology of neurons in Dex group was obviously regular, and the number of normal neurons was increased. Conclusion Intrathecal injection of right metomidine can improve the motor function of lower limbs after ischemia-reperfusion by up-regulating the expression of Beclin1,LC3 protein and gene and increasing the autophagy of neurons.
【作者單位】： 中國(guó)醫(yī)科大學(xué)附屬第一醫(yī)院麻醉科;
【基金】：遼寧省科學(xué)技術(shù)計(jì)劃項(xiàng)目(2012408002) 國(guó)家自然科學(xué)基金(81271370)
【分類號(hào)】：R614

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 孫斌;曹起龍;;鞘內(nèi)注射藥物發(fā)生意外5例分析[J];人民軍醫(yī);1986年12期

2 趙英豪;超常量MTX鞘內(nèi)注射致昏迷、抽搐搶救成功一例[J];中華血液學(xué)雜志;1994年07期

3 盧發(fā)森;鞘內(nèi)注射過(guò)量鏈霉素致急性昏迷1例[J];中國(guó)實(shí)用內(nèi)科雜志;2003年07期

4 陳美;鞘內(nèi)注射化療的護(hù)理[J];交通醫(yī)學(xué);2003年05期

5 丁現(xiàn)超;鄧建中;夏玉彬;張琰;孟君霞;李新剛;;鞘內(nèi)注射甲氨蝶呤致嚴(yán)重化學(xué)性腦膜炎四例分析[J];中國(guó)藥物與臨床;2007年09期

6 趙立雙;郝長(zhǎng)來(lái);;鞘內(nèi)注射甲氨蝶呤致神經(jīng)系統(tǒng)毒性反應(yīng)1例[J];山東醫(yī)藥;2006年19期

7 王建國(guó);鞘內(nèi)注射齊考諾泰治療癌癥或艾滋病疼痛有效[J];新醫(yī)學(xué);2004年06期

8 唐靜;王育琴;;長(zhǎng)春新堿誤行鞘內(nèi)注射致嚴(yán)重神經(jīng)損害及預(yù)防[J];藥物不良反應(yīng)雜志;2007年06期

9 韓旭;;鞘內(nèi)注射藥物治療手部腱鞘炎概述[J];中國(guó)民康醫(yī)學(xué);2012年24期

10 張群強(qiáng),彭孝廉;鞘內(nèi)注射MTX過(guò)量一例臨床觀察[J];臨床內(nèi)科雜志;1989年03期

相關(guān)會(huì)議論文 前5條

1 韓英;趙偉;袁云枝;;鏈霉素鞘內(nèi)注射后發(fā)生可逆性截癱1例[A];2005年中國(guó)防癆協(xié)會(huì)全國(guó)學(xué)術(shù)會(huì)議論文集[C];2005年

2 崔陽(yáng);;鞘內(nèi)注射藥物治療全身活動(dòng)不明顯的神經(jīng)精神狼瘡[A];中華醫(yī)學(xué)會(huì)全國(guó)風(fēng)濕病學(xué)年會(huì)論文匯編[C];2003年

3 傅志儉;張維;謝s，

本文編號(hào)：2459033