【摘要】：目的研究在腦缺血過程中HIF-1α、VEGF因子的表達及其作用機制。方法選取60只成年健康雄性SD大鼠,體重250~300g,清潔級。將大鼠隨機分為兩組:實驗組和對照組,每組各30只。造模取得成功2h后,實驗組注射HIF-1α質(zhì)粒液,對照組注射同等量的PBS緩沖液,按照注射后時間不同分為6h,24h,48h,72h,7d五個時間點,每個時間點亞組為6只大鼠。采用TTC染色法觀察各組大鼠腦梗塞面積,來判斷腦組織缺血程度;采用免疫印跡試驗檢測腦組織梗塞區(qū)中HIF-1α和VEGF的蛋白表達水平。結(jié)果1 TTC染色法觀察各組大鼠腦梗塞面積與對照組相比,實驗組6h、24h、48h時間點上梗塞面積無明顯差異(P0.05),而在72h、7d時間點上有差異,其中7d時差異更為顯著(P0.01)。2注射HIF-1α質(zhì)粒液對大鼠死亡率的影響大鼠在缺血再灌注后注射HIF-1α質(zhì)粒液,在4h~60h之間死亡率最高,若存活時間超過72h死亡率低(P0.01)。3免疫印跡試驗檢測腦組織梗死區(qū)中HIF-1α和VEGF兩種因子其蛋白的表達結(jié)果對照組和實驗組中兩種蛋白表達量在6h、24h逐漸增高(P0.01),24h時表達量最高,48h、72h、7d時逐漸下降,7d時表達量最低(P0.01)。與對照組相比,實驗組各個時間點HIF-1α和VEGF蛋白表達量均升高,具有統(tǒng)計學意義(P0.05)。對HIF-1α、VEGF兩種蛋白表達水平進行相關性分析,結(jié)果表明VEGF和HIF-1α的表達具有正相關性。結(jié)論1 HIF-1α質(zhì)粒在大鼠腦缺血再灌注損傷中,有助于改善腦組織缺血梗死區(qū),使其縮小。2在腦缺血再灌注損傷中,HIF-1α、VEGF因子在此調(diào)控過程中起著非常重要的作用。
[Abstract]:Objective to study the expression and mechanism of HIF-1 偽 and VEGF during cerebral ischemia. Methods 60 adult healthy male SD rats weighing 250 g and 300 g were selected. Rats were randomly divided into two groups: experimental group and control group, 30 rats in each group. Two hours after the establishment of the model, the experimental group was injected with HIF-1 偽 granule solution and the control group was injected with the same amount of PBS buffer. According to the different time after injection, the rats were divided into 6 hours, 24 hours, 48 hours, 72 hours. At 7 days, 6 rats in each subgroup were divided into five time points. The cerebral infarction area of each group was observed by TTC staining to determine the degree of cerebral ischemia, and the protein expression of HIF-1 偽 and VEGF in cerebral infarction area was detected by immunoblotting test. Results 1Compared with the control group, there was no significant difference in the infarct area between the experimental group and the control group at 6 h, 24 h, 48 h (P0.05), but at 72 h and 7 d, there was no significant difference in the infarct area between the experimental group and the control group by TTC staining. The effect of injection of HIF-1 偽 granule solution on the mortality of rats was more significant at the 7th day (P0.01). (2) the death rate of rats injected with HIF-1 偽 granule solution after ischemia reperfusion was the highest among 4h~60h. If the survival time was longer than 72 hours, the mortality was low (P0.01). 3 the expression of HIF-1 偽 and VEGF in cerebral infarction area was detected by immunoblotting assay. The expression of two proteins in the control group and the experimental group was 6 hours. The expression level increased gradually at 24 h (P0.01), the highest at 24 h, the highest at 48 h, at 72 h, gradually decreased at 7 d, and the lowest at 7 d (P 0.01). Compared with the control group, the expression of HIF-1 偽 and VEGF protein in the experimental group was significantly higher than that in the control group (P0.05). The expression levels of HIF-1 偽 and VEGF were analyzed. The results showed that there was a positive correlation between the expression of VEGF 偽 and HIF-1 偽. Conclusion (1) HIF-1 偽 plasmid can improve the ischemic infarct area and reduce it in cerebral ischemia reperfusion injury in rats. 2 in cerebral ischemia reperfusion injury, HIF-1 偽 and VEGF factor play a very important role in the regulation of cerebral ischemia reperfusion injury. [WT5HZ] conclusion\?
【學位授予單位】：華北理工大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R614

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