【摘要】：目的:目前COL9A2基因突變對(duì)腰椎間盤退變術(shù)后復(fù)發(fā)率影響的報(bào)道較少,尤其針對(duì)國(guó)內(nèi)人群的結(jié)果并未明確。本研究旨在探討COL9A2基因多態(tài)性對(duì)腰椎間盤退變術(shù)后復(fù)發(fā)率的相關(guān)影響。方法:納入2013年1月至2014年6月在本院經(jīng)受手術(shù)治療的146名(77名男性,69名女性)腰椎間盤退變患者,將患者分為兩組,其中74名患者(39名男性,35名女性,平均年齡49.8歲)在第一次手術(shù)后24個(gè)月內(nèi)病情復(fù)發(fā),另一組72名患者(38名男性,34名女性,平均年齡49.7歲)在手術(shù)治療后24個(gè)月內(nèi)未出現(xiàn)復(fù)發(fā)情況。通過術(shù)后隨訪采集,經(jīng)患者知情同意后,收集外周血,并對(duì)其基因擴(kuò)增產(chǎn)物行限制性片段分析。結(jié)果:(1)所有的樣本經(jīng)內(nèi)切酶BsmF1酶切后產(chǎn)物結(jié)果為:復(fù)發(fā)組與無(wú)復(fù)發(fā)組中,分別有71例、68例患者為未含Trp的類型,分別有3例、4例患者為含Trp雜合子類型。(2)限制性內(nèi)切酶BceA1酶切結(jié)果為:復(fù)發(fā)組與無(wú)復(fù)發(fā)組中,分別有10例、2例患者為(Arg/Arg)類型,分別有54例、58例患者為(Gln/Gln)類型,分別有10例、12例患者為(Arg/Gln)類型。(3)與無(wú)復(fù)發(fā)組相比,復(fù)發(fā)組中精氨酸純合子數(shù)量占比更高,有統(tǒng)計(jì)學(xué)意義(P=0.031)。結(jié)論:COL9A2基因多態(tài)性與國(guó)內(nèi)人群腰椎間盤退變術(shù)后早期復(fù)發(fā)存在相關(guān)性。
[Abstract]:Aim: there are few reports about the effect of COL9A2 gene mutation on the postoperative recurrence rate of lumbar intervertebral disc degeneration, especially in domestic population. The aim of this study was to investigate the correlation between COL9A2 gene polymorphism and postoperative recurrence rate of lumbar intervertebral disc degeneration. Methods: 146 patients (77 males and 69 females) with lumbar disc degeneration underwent surgical treatment in our hospital from January 2013 to June 2014 were divided into two groups: 74 patients (39 males and 35 females). The mean age was 49.8 years old) within 24 months after the first operation. In another group, 72 patients (38 males, 34 females, mean age 49.7 years) did not recur within 24 months after the first operation, and the other 72 patients (38 males and 34 females, mean age 49.7 years) did not recur within 24 months after the first operation. After follow-up, the peripheral blood was collected with the informed consent of the patients, and the amplified products were analyzed by restriction fragment analysis. Results: (1) all the samples were digested by endonuclease BsmF1 and the results were as follows: 71 cases of recurrence group and no recurrence group, 68 cases of Trp-free type, 3 cases of non-recurrence group, 3 cases of non-recurrence group and 3 cases of non-recurrence group, respectively. Trp heterozygote was found in 4 patients. (2) the results of restriction endonuclease BceA1 digestion showed that there were 10 cases in recurrence group and no recurrence group, 2 cases were Arg/Arg type, 54 cases were Gln/Gln type, 58 cases were Gln/Gln type, and 5 cases in non-recurrence group, 2 cases were Arg/Arg type, 58 cases were Gln/Gln type, 2 cases were Arg/Arg type, 58 cases were Gln/Gln type, and 2 cases were Gln/Gln type. (3) compared with the non-recurrent group, the percentage of arginine homozygotes was higher in the recurrent group than that in the non-recurrent group (P < 0. 031). (3) the number of arginine homozygotes in the recurrent group was higher than that in the non-recurrent group (P < 0. 031). Conclusion: there is a correlation between COL9A2 gene polymorphism and early recurrence of lumbar intervertebral disc degeneration in China.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R687.3

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