【摘要】：背景:傳統(tǒng)觀念認(rèn)為哺乳動(dòng)物的腦和脊髓損傷后不能再生,脊髓損傷后功能恢復(fù)的預(yù)后希望很渺茫。目的:分析經(jīng)顱磁刺激聯(lián)合嗅鞘細(xì)胞移植治療大鼠脊髓損傷的修復(fù)效果。方法:對(duì)80只大鼠進(jìn)行脊髓損傷模型制備,隨機(jī)等分成4組,胸髓橫斷損傷組、嗅鞘細(xì)胞移植組、重復(fù)經(jīng)顱磁刺激組及聯(lián)合組。各組大鼠行T10全椎板切除建立胸髓橫斷損傷動(dòng)物模型,造模24 h后,嗅鞘細(xì)胞移植組和聯(lián)合組大鼠在損傷區(qū)附近注射嗅鞘細(xì)胞,重復(fù)經(jīng)顱磁刺激組及聯(lián)合組大鼠于左前囟上方接受重復(fù)經(jīng)顱磁刺激治療。結(jié)果與結(jié)論:移植4周后,與其他3組相比,重復(fù)經(jīng)顱磁刺激聯(lián)合嗅鞘細(xì)胞移植組大鼠運(yùn)動(dòng)功能恢復(fù)明顯,運(yùn)動(dòng)功能BBB評(píng)分升高,坐骨神經(jīng)電生理功能明顯恢復(fù),損傷脊髓修復(fù)較好。提示重復(fù)經(jīng)顱磁刺激聯(lián)合嗅鞘細(xì)胞移植治療大鼠脊髓損傷可以獲得顯著療效,兩者聯(lián)合應(yīng)用可明顯促進(jìn)脊髓損傷后的軸突再生及功能恢復(fù)。
[Abstract]:Background: the traditional view is that mammalian brain and spinal cord injury can not regenerate, and the prognosis of functional recovery after spinal cord injury is very poor. Objective: to analyze the effect of transcranial magnetic stimulation combined with olfactory ensheathing cell transplantation in the treatment of spinal cord injury in rats. Methods: 80 rats were randomly divided into 4 groups: thoracic spinal cord injury group, olfactory ensheathing cell transplantation group, repetitive transcranial magnetic stimulation group and combined group. Rats in each group were treated with T10 total laminectomy to establish an animal model of thoracic spinal cord transection injury. After 24 hours, the olfactory ensheathing cell transplantation group and the combined group were injected with olfactory ensheathing cells near the injured area. Repeated transcranial magnetic stimulation was performed on the left anterior fontanelle of rats in the combined group and repeated transcranial magnetic stimulation group. Results and conclusion: four weeks after transplantation, compared with the other three groups, the rats in the group of repeated transcranial magnetic stimulation combined with olfactory ensheathing cells showed obvious recovery of motor function, increased BBB score of motor function, and obvious recovery of electrophysiological function of sciatic nerve in the group of repeated transcranial magnetic stimulation and olfactory ensheathing cell transplantation. The injured spinal cord was well repaired. The results suggest that repeated transcranial magnetic stimulation combined with olfactory ensheathing cell transplantation can obtain significant therapeutic effect on spinal cord injury in rats. Combined use of both can significantly promote axon regeneration and functional recovery after spinal cord injury.
【作者單位】： 大連市骨科醫(yī)院;
【基金】：大連市科技發(fā)展計(jì)劃支持項(xiàng)目(2013E15SF168)~~
【分類號(hào)】：R651.2

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