【摘要】：目的通過建立肺挫傷模型,研究肺挫傷早期血清炎癥因子TNF-α、IL-1β、IL-10、IL-13與肺超微結(jié)構(gòu)以及肺功能的變化方法將16只新西蘭白兔隨機(jī)分為:(1)嚴(yán)重胸外傷肺挫傷組(n=12);(2)正常無損傷對(duì)照組(n=4)。建立嚴(yán)重胸外傷肺挫傷模型,于挫傷后1、2、3、4h分別檢測(cè)血清IL-10、IL-13、IL-1β及TNF-α含量,同時(shí)在上述各個(gè)時(shí)相獲取呼吸頻率f、潮氣量(VT)、氣道壓力、呼氣末CO2濃度及呼吸流率曲線的數(shù)據(jù),在各時(shí)相解剖肺組織,觀察其超微結(jié)構(gòu)病理的改變結(jié)果肺挫傷后早期TNF-α呈高表達(dá)狀態(tài),IL-1β表達(dá)升高,IL-10、IL-13表達(dá)總體上持續(xù)下降,肺超微結(jié)構(gòu)進(jìn)行性破壞,最終肺泡壁損害,肺泡腔內(nèi)及間隔間大量紅細(xì)胞淤積及炎性細(xì)胞滲出,毛細(xì)血管充血嚴(yán)重,導(dǎo)致肺泡通氣量及PO2明顯下降,P-AO2持續(xù)加大,肺順應(yīng)性下降。結(jié)論肺挫傷早期血清IL-1β、TNF-α表達(dá)明顯升高,IL-10、IL-13表達(dá)降低;肺挫傷早期肺組織超微結(jié)構(gòu)進(jìn)行性破壞,肺功能持續(xù)降低;肺挫傷早期血清炎癥因子的變化與肺超微結(jié)構(gòu)及肺功能的改變可能有一定相關(guān)性
[Abstract]:Objective to study the serum inflammatory factors TNF- 偽, IL-1 尾 and IL-10, in early stage of lung contusion by establishing pulmonary contusion model. Methods 16 New Zealand white rabbits were randomly divided into two groups: (1) Pulmonary contusion group (n = 12); (2) normal control group (n = 4). The lung contusion model of severe thoracic trauma was established. The levels of serum IL-10,IL-13,IL-1 尾 and TNF- 偽 were measured at 1h, 2h, 3h after contusion, and the respiratory frequency f and tidal volume (VT), airway pressure were obtained at the same time. The data of end-expiratory CO2 concentration and respiratory flow rate curve were used to anatomize the lung tissue at each phase. The ultrastructural and pathological changes of the lung tissue were observed. The results showed that the expression of TNF- 偽 was high, the expression of IL-1 尾 was increased, and the expression of IL-10, was increased in the early stage of pulmonary contusion. The expression of IL-13 decreased continuously, the ultrastructure of lung was destroyed gradually, the alveolar wall was damaged, a large number of red blood cells and inflammatory cells exudated in and between alveolar cavity and septum, and the capillary congestion was serious. As a result, alveolar ventilation volume and PO2 decreased significantly, P-AO2 continued to increase and lung compliance decreased. Conclusion the expression of IL-1 尾 and TNF- 偽 in serum increased significantly and the expression of IL-10,IL-13 decreased in the early stage of pulmonary contusion, and the ultrastructure of lung tissue was gradually destroyed and the lung function was continuously decreased in the early stage of pulmonary contusion. The changes of serum inflammatory factors may be related to the changes of pulmonary ultrastructure and pulmonary function in the early stage of pulmonary contusion.
【學(xué)位授予單位】：廣州醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R655.3

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