【摘要】：[目的]觀察過(guò)表達(dá)Foxc2基因?qū)ν霉撬栝g充質(zhì)干細(xì)胞(BMSCs)增殖及成骨分化的影響。[方法]構(gòu)建攜帶Foxc2和綠色熒光蛋白(GFP)基因的重組慢病毒表達(dá)載體,分離、培養(yǎng)兔BMSCs,分別以Lenti-GFP(GFP組)、Lenti-Foxc2(Foxc2組)轉(zhuǎn)染BMSCs,另設(shè)未轉(zhuǎn)染BMSCs對(duì)照組。MTT法檢測(cè)過(guò)表達(dá)Foxc2對(duì)細(xì)胞增殖能力;Western blot、Real time PCR檢測(cè)轉(zhuǎn)染后7d各組細(xì)胞Foxc2蛋白、m RNA表達(dá);以及轉(zhuǎn)染后1、2、4周各組細(xì)胞骨鈣蛋白(OCN)、骨橋蛋白(OPN)、一型膠原(COLⅠ)蛋白及m RNA表達(dá);轉(zhuǎn)染后2周,各組行堿性磷酸酶(ALP)染色和ALP活性檢測(cè)。[結(jié)果]Foxc2組Foxc2 m RNA和蛋白高效表達(dá);對(duì)照組、GFP組無(wú)Foxc2 m RNA及蛋白表達(dá);MTT法檢測(cè)示過(guò)表達(dá)Foxc2對(duì)BMSCs的增殖未產(chǎn)生顯著影響;Foxc2組OCN、OPN、COLⅠm RNA和蛋白表達(dá)顯著高于其他組(P0.01),余組比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);Foxc2組ALP染色陽(yáng)性區(qū)域大于其余兩組;ALP活性顯著高于其他各組(P0.01)。[結(jié)論]過(guò)表達(dá)Foxc2不影響細(xì)胞增殖,可持續(xù)表達(dá)基因產(chǎn)物,并可促進(jìn)BMSCs向成骨細(xì)胞分化。
[Abstract]:[objective] to observe the effect of overexpression of Foxc2 gene on (BMSCs) proliferation and osteogenic differentiation of rabbit bone marrow mesenchymal stem cells (BMSCs). [methods] Recombinant lentivirus expression vector carrying Foxc2 and green fluorescent protein (GFP) gene was constructed. Rabbit BMSCs, was isolated and transfected into BMSCs, with Lenti-GFP (GFP group) and Lenti-Foxc2 (Foxc2 group) respectively. In addition, untransfected BMSCs was used as control group. MTT assay was used to detect the ability of Foxc2 expression on cell proliferation. The expression of Foxc2 protein, m RNA and osteocalcin (OCN), (OCN), osteopontin type I collagen (COL 鈪，

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