【摘要】：目的觀察富血小板血漿(PRP)凝膠及腺病毒介導(dǎo)的骨形態(tài)發(fā)生蛋白2(BMP-2)基因轉(zhuǎn)染對(duì)兔骨髓間充質(zhì)干細(xì)胞(BMSCs)體外成軟骨分化的影響。方法取新西蘭大白兔骨髓培養(yǎng)BMSCs,抽取靜脈血制備PRP,以Ad-GFP-hBMP-2(BMP-2-BMSCs組)及Ad-GFP(對(duì)照組)轉(zhuǎn)染的BMSCs分別與PRP凝膠復(fù)合,繼續(xù)培養(yǎng)。用掃描電鏡、MTT檢測PRP凝膠的生物相容性,RT-PCR檢測各組的Ⅰ型膠原、Ⅱ型膠原、Ⅹ型膠原、蛋白聚糖、SOX-9表達(dá)。結(jié)果掃描電鏡及MTT檢測證實(shí)PRP凝膠具有良好的生物相容性。RT-PCR分析表明,單層培養(yǎng)的BMP-2-BMSCs組的Ⅱ型膠原、蛋白聚糖和SOX-9的表達(dá)水平均高于對(duì)照組(P0.05),但Ⅰ型膠原表達(dá)水平較對(duì)照組明顯下降(P0.05),同時(shí)Ⅹ型膠原表達(dá)水平與對(duì)照組相比差異無統(tǒng)計(jì)學(xué)意義(P0.05);與PRP復(fù)合后,BMP-2-BMSCs組的Ⅱ型膠原、蛋白聚糖和SOX-9表達(dá)水平較對(duì)照組升高(P0.05),而Ⅰ型膠原和Ⅹ型膠原表達(dá)水平與對(duì)照組比較差異無統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論轉(zhuǎn)染BMP-2的BMSCs在PRP凝膠中生長良好,BMP-2基因轉(zhuǎn)染能促進(jìn)BMSCs的體外成軟骨分化。
[Abstract]:Objective to investigate the effects of platelet-rich plasma (PRP) gel and adenovirus-mediated bone morphogenetic protein 2 (BMP-2) gene transfection on chondrogenic differentiation of rabbit bone marrow mesenchymal stem cells (BMSCs) in vitro. Methods New Zealand white rabbit bone marrow culture (BMSCs,) was used to extract venous blood to prepare PRP,. BMSCs transfected with Ad-GFP-hBMP-2 (BMP-2-BMSCs group) and Ad-GFP (control group) were combined with PRP gel respectively. The biocompatibility of PRP gel was detected by scanning electron microscope (SEM) and MTT. The expression of type 鈪，

本文編號(hào)：2304915