【摘要】：目的： 目前胰島移植在國(guó)際上已經(jīng)逐漸從實(shí)驗(yàn)室技術(shù)轉(zhuǎn)化為臨床治療手段,并取得了令人滿意的效果。本實(shí)驗(yàn)通過(guò)消化心臟死亡供體胰腺器官獲取足量質(zhì)優(yōu)的胰島細(xì)胞團(tuán)以用于胰島純化,進(jìn)而實(shí)現(xiàn)臨床胰島移植,達(dá)到治愈糖尿病的目的。 實(shí)驗(yàn)方法： 通過(guò)專業(yè)的器官獲取團(tuán)隊(duì)獲取心臟死亡供體胰腺,保存于4℃器官保存液(UW液或HTK液)中；送達(dá)實(shí)驗(yàn)室后,由專業(yè)團(tuán)隊(duì)修剪胰腺,去除胰周組織,暴露胰管；胰管高壓灌注胰腺消化液10分鐘；基于膠原酶以半自動(dòng)人胰腺分離裝置消化胰腺組織,獲取胰島細(xì)胞團(tuán)；通過(guò)DTZ染色后進(jìn)行胰島計(jì)數(shù),并評(píng)估胰島純度,以用于進(jìn)一步的胰島純化、培養(yǎng)和功能鑒定。 結(jié)果： 通過(guò)器官獲取團(tuán)隊(duì)獲取10條優(yōu)質(zhì)胰腺,胰腺平均質(zhì)量為56.9±24.1g,10條胰腺灌注效果良好,對(duì)10條胰腺的消化,平均獲取712068±459623個(gè)胰島,平均胰島當(dāng)量為635458±510168IEQ,以用于胰島的純化。 結(jié)論： 通過(guò)實(shí)驗(yàn)建立了一套可靠地胰腺消化系統(tǒng),獲取了胰島分離的成功,為下一步的胰島純化、功能鑒定,以及更進(jìn)一步的胰島移植奠定了堅(jiān)實(shí)的基礎(chǔ)。
[Abstract]:Objective: at present, islet transplantation has been gradually transformed from laboratory technique to clinical treatment in the world, and satisfactory results have been obtained. In this experiment, sufficient and high quality islet cell clusters were obtained by digesting the pancreatic organs of cardiac death donor for islet purification, and then clinical islet transplantation was achieved, and the goal of curing diabetes was achieved. Methods: the pancreas of cardiac death donor was obtained by professional organ acquisition team and stored in 4 鈩，

本文編號(hào)：2294660