發(fā)布時(shí)間：2018-10-09 14:34

【摘要】：目的血管活性腸肽(VIP)調(diào)控骨折愈合的作用機(jī)制和信號(hào)通路機(jī)制不明,文章通過(guò)建立選擇性切斷大鼠感覺(jué)/運(yùn)動(dòng)神經(jīng)聯(lián)合脛骨骨折的動(dòng)物模型,探討周圍神經(jīng)損傷后其骨折愈合過(guò)程中VIP對(duì)微血管密度(MVD)的影響。方法將60只SD實(shí)驗(yàn)大鼠隨機(jī)數(shù)字表法分成3組:脛骨骨折組,感覺(jué)神經(jīng)切斷組(脛骨骨折+后根感覺(jué)神經(jīng)切斷組),運(yùn)動(dòng)神經(jīng)切斷組(脛骨骨折+前根運(yùn)動(dòng)神經(jīng)切斷組),每組20只。3組大鼠在模型建立后的1、2、3、4周處死大鼠,骨痂標(biāo)本在骨折處周圍5mm處獲取,觀察VIP的表達(dá)及MVD的變化規(guī)律。結(jié)果組織學(xué)觀察顯示,感覺(jué)神經(jīng)切斷組在4個(gè)時(shí)相點(diǎn)均較脛骨骨折組和運(yùn)動(dòng)神經(jīng)切斷組新生骨小梁成熟度低;免疫組化結(jié)果顯示,與第2、3周感覺(jué)神經(jīng)切斷組骨痂VIP平均光密度(0.156±0.015、0.163±0.012)比較,脛骨骨折組(0.216±0.012、0.223±0.010)、運(yùn)動(dòng)神經(jīng)切斷組(0.198±0.014、0.212±0.016)明顯升高(P0.05)。免疫組化檢測(cè)骨痂MVD發(fā)現(xiàn),與感覺(jué)神經(jīng)切斷組第2、3周骨痂MVD平均密度(26.4±2.2、32.3±2.0)比較,脛骨骨折組(38.2±2.3、40.6±2.6)、運(yùn)動(dòng)神經(jīng)切斷組(36.6±2.2、38.5±2.1)明顯升高(P0.05)。術(shù)后第2、3、4周,與感覺(jué)神經(jīng)切斷組比較,運(yùn)動(dòng)神經(jīng)切斷組和脛骨骨折組脛骨濕重明顯升高(P0.05),其中以2周時(shí)增加幅度最為顯著。結(jié)論失感覺(jué)神經(jīng)支配比失運(yùn)動(dòng)神經(jīng)支配對(duì)骨痂內(nèi)MVD的形成、骨痂成熟度、骨折愈合速度的影響更為明顯。
[Abstract]:Objective to investigate the mechanism of vasoactive intestinal peptide (VIP) regulating fracture healing and the mechanism of signal pathway. To investigate the effect of VIP on microvessel density (MVD) during fracture healing after peripheral nerve injury. Methods 60 SD experimental rats were randomly divided into 3 groups: tibial fracture group, tibial fracture group, Sensory nerve transection group (posterior root sensory nerve transection group), motor nerve transection group (tibial fracture anterior root motor nerve transection group), each group of 20 rats in each group of 20 rats after the establishment of the model 1 2 weeks after the death of rats, Callus specimens were obtained from the 5mm around the fracture to observe the expression of VIP and the change of MVD. Results histological observation showed that the maturity of trabeculae in sensory nerve transection group was lower than that in tibial fracture group and motor nerve transection group. The average optical density of callus (0.156 鹵0.015 鹵0.163 鹵0.012) was significantly higher in the tibial fracture group (0.216 鹵0.012 鹵0.223 鹵0.010) than in the motor nerve transection group (0.198 鹵0.014 鹵0.212 鹵0.016), compared with that in the sensory nerve transection group at the 2nd week (0.156 鹵0.015 鹵0.163 鹵0.012) (P0.05). Compared with the sensory nerve transection group (26.4 鹵2.2 鹵32.3 鹵2.0), the average density of callus MVD was significantly higher in the tibial fracture group (38.2 鹵2.3 鹵40.6 鹵2.6) and the motor nerve transection group (36.6 鹵2.2 鹵38.5 鹵2.1) than in the sensory nerve transection group (26.4 鹵2.2 鹵32.3 鹵2.0). The wet weight of tibia in motor nerve transection group and tibial fracture group was significantly higher than that in sensory nerve transection group (P0.05), especially at 2 weeks. Conclusion the effects of denervation on the formation of MVD, callus maturity and fracture healing speed are more obvious than those of motor innervation.
【作者單位】： 贛南醫(yī)學(xué)院第一附屬醫(yī)院骨科;贛南醫(yī)學(xué)院生物化學(xué)與分子生物學(xué)教研室;
【基金】：江西省衛(wèi)生計(jì)生委科技計(jì)劃課題(20155451) 贛州市指導(dǎo)性科技計(jì)劃項(xiàng)目(GZ2016ZSF203) 贛南醫(yī)學(xué)院科研課題(QD201604)
【分類號(hào)】：R683

【參考文獻(xiàn)】

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