【摘要】：目的:評(píng)價(jià)ATP敏感性鉀通道(KATP通道)在羥考酮后處理減輕大鼠心肌缺血再灌注損傷中的作用。方法:成年健康雄性SD大鼠50只,8~12周齡,體重200~300 g,采用隨機(jī)數(shù)字表法分為5組(n=10):假手術(shù)組(S組)、缺血再灌注組(I/R組)、羥考酮后處理組(OP組)、羥考酮聯(lián)合κ受體阻斷劑nor-binaltorphimine(Nor-BNI)組(ON組)和KATP通道阻斷劑格列苯脲(Glibenclamide)+羥考酮后處理組(GOP組)。采用結(jié)扎冠狀動(dòng)脈前降支30 min,再灌注120 min的方法制備大鼠心肌缺血再灌注損傷模型,S組只穿線,不結(jié)扎左冠狀動(dòng)脈前降支。ON組與GOP組分別于缺血前頸靜脈注射Nor-BNI 2mg/kg和格列苯脲1mg/kg;OP組、ON組與GOP組于再灌注前5min時(shí)頸靜脈注射羥考酮0.5mg/kg,S組和I/R組給予等容量生理鹽水。各組于再灌注120 min時(shí)采集頸動(dòng)脈血樣,檢測血清肌鈣蛋白I(cTnI)和乳酸脫氫酶(LDH)的濃度。然后處死大鼠,取左心室,確定心肌梗死體積。結(jié)果:與S組比較,I/R組、OP組、ON組和GOP組血清中cTnI濃度、LDH濃度和心肌梗死體積升高(P0.05);與I/R組比較,OP組、ON組和GOP組血清c TnI濃度、LDH濃度和心肌梗死體積降低(P0.05);與OP組比較,ON組和GOP組血清cTnl濃度、LDH濃度和心肌梗死體積升高(P0.05)。結(jié)論:KATP通道的開放參與了羥考酮后處理減輕大鼠心肌缺血再灌注損傷。
[Abstract]:Aim: to evaluate the role of ATP sensitive potassium channel (KATP channel) in relieving myocardial ischemia reperfusion injury in rats after treatment with hydroxycodone. Methods: 50 healthy male SD rats were 12 weeks old. Weighing 200,300 g, the rats were randomly divided into five groups (n = 10): sham operation group (S group), ischemia reperfusion group (I / R group), hydroxycodone post-treatment group (OP group), hydroxycodone plus 魏 receptor blocker nor-binaltorphimine (Nor-BNI) group (ON group) and KATP channel blocker glibenclamide (KATP channel blocker). (Glibenclamide) hydroxycodone group (GOP group). Rat myocardial ischemia reperfusion injury model was established by ligating anterior descending coronary artery for 30 min, and reperfusion for 120 min. No ligation of left anterior descending coronary artery. On group and GOP group were injected with Nor-BNI 2mg/kg and glibenclamide 1 mg / kg op group before ischemia. On group and GOP group were given equal volume saline in jugular vein injection of hydroxycodone 0.5 mg / kg / kg S and I / R group respectively during 5min before reperfusion. Carotid blood samples were collected at 120 min after reperfusion. Serum troponin I (cTnI) and lactate dehydrogenase (LDH) were measured. Then the rats were killed and the left ventricle was taken to determine the volume of myocardial infarction. Results: compared with S group, serum cTnI concentration and myocardial infarction volume in op group and GOP group were increased (P0.05), c TnI concentration and myocardial infarction volume were decreased in op group (P0.05) and in GOP group (P0.05). Serum cTnl concentration and myocardial infarction volume were higher than those in on group and GOP group (P 0.05). Conclusion the opening of the K-ATP channel may play an important role in reducing myocardial ischemia reperfusion injury after treatment with hydroxycodone in rats.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R614

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