【摘要】：目的探討丙泊酚對在體小鼠小腦皮層浦肯野細(xì)胞自發(fā)性單純性放電活動、自發(fā)性復(fù)雜峰電位放電特性的影響。方法選用6~8周齡ICR小鼠6只,腹腔注射烏拉坦麻醉,在小腦CrusⅡ小葉行開顱手術(shù),開孔直徑為1.0~1.5 mm,去除硬腦膜后,用蠕動泵在腦表面持續(xù)灌流含氧的人工腦脊液,浦肯野細(xì)胞貼附式記錄使用Multi clamp700B膜片鉗放大器,通過D/A轉(zhuǎn)換器1440和Clampfit 10.3軟件獲取浦肯野細(xì)胞自發(fā)性活動數(shù)據(jù)。待自發(fā)性活動記錄穩(wěn)定后,給予丙泊酚,觀察其對浦肯野細(xì)胞自發(fā)性單純峰電位(SS)和復(fù)雜峰電位(CS)活動的影響。結(jié)果腦表面給予丙泊酚劑量依存地抑制小鼠小腦浦肯野細(xì)胞自發(fā)性SS放電活動,其半數(shù)抑制濃度為143μmol/L,當(dāng)濃度為500μmol/L時(shí)自發(fā)性SS放電完全抑制,然而,丙泊酚對自發(fā)性復(fù)雜峰電位放電頻率和波形特征無顯著影響。結(jié)論在體條件下,腦表面灌流丙泊酚顯著抑制浦肯野細(xì)胞自發(fā)性SS放電活動。
[Abstract]:Objective to investigate the effect of propofol on spontaneous single discharge and spontaneous complex peak discharge in mouse cerebellar cortex Purkinje cells. Methods six ICR mice aged 6 weeks, 8 weeks old, were anesthetized by intraperitoneal injection of uratan. Craniotomy was performed in the cerebellar Crus 鈪，

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